AI Summary & Key Takeaways
AI InsightThese observations may clarify the role of PVs in the mechanisticassociation between hypertension and AF.
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Özet
Objective: Hypertension is a significant risk factor for atrial fibrillation (AF). The role of pulmonary vein (PV) remodeling in the mechanistic associationbetween hypertension and AF is not definitive. In this study, we aimed to identify changes in the electrophysiology and histology in PVsin two-kidney, one-clip (2K1C) hypertensive rats.Methods: Fifty male Sprague-Dawley rats were classified into the 2K1C and sham-operated groups. The systolic blood pressure was measuredevery 2 weeks. The left atrial diameter was measured by transthoracic echocardiography. Left superior PV (LSPV) and left atrial (LA) fibrosis wasevaluated by Masson’s trichrome staining. The expression of fibrosis markers [angiotensin II (Ang II), transforming growth factor-β1 (TGF-β1),matrix metalloproteinase-2 (MMP-2), and collagen I (Col I)] and ion channels [Kir2.1, Kir2.3, Cav1.2, and Nav1.5] in LSVP was quantified by westernblot. Conventional microelectrodes were used to record the action potential duration at 90% repolarization (APD90) and effective refractoryperiod (ERP) in isolated LA.Results: At 4 months, the 2K1C hypertensive rats developed LA dilation. Col deposition in LSPV and left atrium and expression of TGF-β1, MMP-2,and Col I in LSPV were significantly increased in 2K1C hypertensive rats. In addition, hypertension reduced the expression of Nav1.5 and Kir2.1,although there were no significant differences in APD90; ERP; and expression of Ang II, Kir2.3, and Cav1.2 between the two groups.Conclusion: Hypertension may lead to changes in the electrophysiology and histology of rats PVs, which is characterized by significant reductionin the expression of Nav1.5 and Kir2.1 and increase in interstitial fibrosis. These observations may clarify the role of PVs in the mechanisticassociation between hypertension and AF.