SavedAdd your journal

Journals / ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi / 2001 / Cilt: 11 - Sayı: 2

Identification of some wheat commercial varieties and advanced lines by biochemical markers

Bazı buğday tescilli çeşit ve ileri hatlarının biyokimyasal markörler ile tanımlanması

Gün KIRCALIOĞLU
Pages
1–19
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to the results, it is clear that wheat lines and varieties of breeding programs could be identified with glütenin banding patterns.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The objective of this study was to identify durum wheat genotypes by using glütenin protein as a molecular marker.
📊
Key Findings & Data:Glütenin banding patterns of 18 durum wheat genotypes from CIMMTT collections were obtained by using the SDS-PAGE technique.
💡
Conclusion & Impact:According to the results, it is clear that wheat lines and varieties of breeding programs could be identified with glütenin banding patterns.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:SDS-PAGE · makarnalık buğday · glutenin

Abstract

The objective of this study was to identify durum wheat genotypes by using glütenin protein as a molecular marker. Glütenin banding patterns of 18 durum wheat genotypes from CIMMTT collections were obtained by using the SDS-PAGE technique. Evaluation of these glütenin banding patterns showed that these genotypes were totaly different from each other and variation among genotypes could be expressed by these patterns. According to the results, it is clear that wheat lines and varieties of breeding programs could be identified with glütenin banding patterns. However, no similarity was observed in the banding patterns of the genotypes in the same pedigree groups.

Özet

Makarnalık buğday genotiplerinin farklılıklarının tanımlanması amacıyla yapılan bu araştırmada glütenin proteini moleküler markör olarak kullanılmıştır. CIMMYT'ten sağlanan toplam 18 makarnalık buğday genotipinin sodyum dodesil sülfat poliakrilamid.jel elektroforezi (SDS-PAGE) tekniği ile glütenin bantları elde edilmiştir. Çalışmamızda elde edilen sonuçlara göre, materyali oluşturan genotiplerin tamamı kendilerine özgü glütenin bant desenleri oluşturmuşlardır. Çalışmada kullanılan makarnalık buğday genotiplerinin (buğday çeşit ve ileri hatları) birbirlerinden oldukça farklı glütenin bant desenleri oluşturduğu görülmektedir. Literatür bildirişlerine göre glütenin bant desenleri ile buğday ıslah programlarında yer alan hatların ve çeşitlerin tanımlamaları yapılabilmektedir. Ancak aynı pedigri grubunda yer alan genotiplere ait glütenin bant desenleri incelendiğinde bunların genotiplerde mevcut olan benzerliği ortaya koyamadığı gözlenmiştir.

🔖

Cite This Article

KIRCALIOĞLU, G. (2001). Identification of some wheat commercial varieties and advanced lines by biochemical markers. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi, Cilt: 11 - Sayı: 2, 1–19. https://ajindex.com/articles/356437

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (5)

  1. Anonymous. 1995. Sigma. Biochemicals Organic Compounds and Diagnostic Reagerts. Sigma Chemical Company. St Louis, MO, USA. Bietz, J. A., and J. S. Wall. 1972. Wheat gluten subunits: Molecular weights determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Cereal chem. 49: 416-432.
  2. Bietz, J. A. 1987. Wheat and wheat improvement agronomy. Monograph no: 13 (2nd edition). Brash, S. B. 1985. Ethno biologists Comb Turkey's Rich Wheat Trove in Search of Patterns of Diversity. Diversity, vol. 3 No 1, 2. Cerny J., A Sasek, J. Kubanek, and J. Maly. 1989. Electrophoretic spectra of gliadins and high molecular weight glütenin subunits of some winter wheat (Triticum aestivum L.) varieties from the world collection. Sbornik-UVTIZ,-Potravinarske-Vedy, 1989, 7:1, 15-27; 20 ref. Chaudhry M. A. 1992. Identification of some wheat varieties by the electroforetic analyses of gliadin proteins. Doktora tezi, Ankara üniversitesi, Fen Bilimleri Enstitüsü. Ciaffi, M., L., D. Lafiandra, and E. Proceddu. 1992. Seed storage proteins of wild wheat progenitors and their relationships with technological properties. Hereditas 116: 315-22.
  3. Ciaffi, M., D. Lafiandra, E. Porceddu, and S. Benedettelli. 1993 a. Storage protein variation in wild emmer wheat (Triticum turgidum ssp. dicoccoides). from Jordan and Turkey. I. Electrophoretic characterization of genotypes. Theor. Apply. Genet, (in press). Ciaffi, M., D, Lafiandra, E. Porceddu, and S. Benedettelli. 1993 b. Storage-protein variation in wild emmer wheat (Triticum turgidum ssp. dicoccoides). from Jordan and Turkey. II. Patterns of allele distribution. Theor. Apply Genet, (in press). Du Cross D. L„ G. J. Lawrence, D. M. Miskelyand, and C. W. Wrigley. 1980. Systematic identification of Australian wheat varieties by laboratory methods (Csiro wheat Res. unit. tech. publ. 7 ). Elton, G. A. H., and J. A. D. Eward. 1966. Glutenins and gliadins electrophoretic studies. J. Agric. Food Chem. 17: 34-38. Huebner F. R. i 970. Comparative studies on glutenins from different classes of wheat. J. Agric. Food Chem. 18: 256-259. Johnsoh B. L., and O. Hall. 1965. Analysis of phylogenetic affinities in the triticinae by protein electrophoresis. Ame. J. Bot. 52: 506-513.
  4. Johnson, B. L. 1972. Seed protein profiles and the origin of the hexaploid wheats. Ag. J. Bot. 59: 952-960.
  5. Kosmolak, F. G„ J. E. Dexter, R. R. Matsuo, D. Leisle, andB. A. Marchylo. 1980. A relationship between durum wheat quality and gliadin electrophoregrams Can. J. Plant.