SavedAdd your journal

Journals / Acta Orthopaedica et Traumatologica Turcica / 2021 / Cilt: 55 - Sayı: 2

Expression and significance of related genes in the early stage of post-traumatic heterotopic ossification in a rat model of Achilles tenotomy

Du YU
Jinyong JU
Feng XUE
Yong ZHAO
Weizhe SHI
Haijun XIAO
Pages
94–101
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this study, we investigated theexpression levels of the related cytokines in the early stages of traumatic HO in the Achilles tendon tenotomy rat model to betterunderstand the pathogenesis of HO.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: This study aims to determine expression profiles of relevant genes in the early stages of post-traumatic heterotopicossification (HO) in a rat model of Achilles tenotomy.Methods: A total of 80 male Sprague-Dawley rats were randomly assigned to two groups: the HO group and the control group.Tenotomy was performed in the Achilles tendon of the rats in the HO group, and no intervention was conducted in the controlgroup.
📊
Key Findings & Data:On the 3rd, 5th, 8th, and 14th days after the operation, 8 rats were taken from each group at each time point, and the Achillestendon and surrounding tissue specimens were collected.
💡
Conclusion & Impact:In this study, we investigated theexpression levels of the related cytokines in the early stages of traumatic HO in the Achilles tendon tenotomy rat model to betterunderstand the pathogenesis of HO.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Özet

Objective: This study aims to determine expression profiles of relevant genes in the early stages of post-traumatic heterotopicossification (HO) in a rat model of Achilles tenotomy.Methods: A total of 80 male Sprague-Dawley rats were randomly assigned to two groups: the HO group and the control group.Tenotomy was performed in the Achilles tendon of the rats in the HO group, and no intervention was conducted in the controlgroup. On the 3rd, 5th, 8th, and 14th days after the operation, 8 rats were taken from each group at each time point, and the Achillestendon and surrounding tissue specimens were collected. Gene expressions of TGF-β, BMP, GDF, IL, and MMP families as wellas TNF-α, HIF-1α chordin, gremlin, noggin, and NODAL were analyzed by qRT-PCR. The relevant genes that were highly expressed at different time points were screened, and immunohistochemical staining was then used to verify their expression. Atthe 10th week, HO formation was explored by radiographic and histological examination in the remaining 8 rats of each group.Results: Both the radiographic and histological analyses indicated that all the rats developed HO in the HO group (100%),whereas no HO occurred in the control group. Surrounding tissues obtained from the HO group showed significantly highergene expressions of TGF-β1, BMP-1, IL1β, HIF-1α, and MMP-2 but lower expressions of BMP-4, GDF-8, and TNF-α comparedwith the control group. In addition, immunohistochemical staining confirmed the higher protein expression levels of relevantgenes in the HO group.Conclusion: TGF-β1, BMP-1, IL-1β, HIF-1α, MMP-2, BMP4, GDF-8, and TNF-α may be associated with the formation of traumatic HO; and BMP4, GDF-8, and TNF-α may play a protective role in the early stage of HO. In this study, we investigated theexpression levels of the related cytokines in the early stages of traumatic HO in the Achilles tendon tenotomy rat model to betterunderstand the pathogenesis of HO.

🔖

Cite This Article

YU, D., JU, J., XUE, F., ZHAO, Y., SHI, W., XIAO, H. (2021). Expression and significance of related genes in the early stage of post-traumatic heterotopic ossification in a rat model of Achilles tenotomy. Acta Orthopaedica et Traumatologica Turcica, Cilt: 55 - Sayı: 2, 94–101. https://ajindex.com/articles/353410

References (13)

  1. Downey J, Lauzier D, Kloen P, et al. Prospective heterotopic ossification progenitors in adult human skeletal muscle. Bone 2015; 71: 164-70
  2. Winkler S, Niedermair T, Fuchtmeier B, et al. The impact of hypoxia on mesenchymal progenitor cells of human skeletal tissue in the pathogenesis of heterotopic ossification. Int Orthop 2015; 39: 2495-501
  3. Anthonissen J, Ossendorf C, Hock JL, et al. The role of muscular trauma in the development of heterotopic ossification after hip surgery: An animal-model study in rats. Injury 2016; 47: 613-6
  4. Burd TA, Hughes MS, Anglen JO. Heterotopic ossification prophylaxis with indomethacin increases the risk of long-bone nonunion. J Bone Joint Surg Br 2003; 85: 700-5
  5. Tozun R, Pınar H, Yeşiller E, Hamzaoğlu A. Indomethacin for prevention of heterotopic ossification after total hip arthroplasty. J Arthroplasty 1992; 7: 57- 61
  6. Mazonakis M, Berris T, Lyraraki E, Damilakis J. Cancer risk estimates from radiation therapy for heterotopic ossification prophylaxis after total hip arthroplasty. Med Phys 2013; 40: 101702
  7. Pavey GJ, Polfer EM, Nappo KE, Tintle SM, Forsberg JA, Potter BK. What risk factors predict recurrence of heterotopic ossification after excision in combat-related amputations? Clin Orthop Relat Res 2015; 473: 2814-24
  8. Toom A, Arend A, Gunnarsson D, et al. Bone formation zones in heterotopic ossifications: histologic findings and increased expression of bone morphogenetic protein 2 and transforming growth factors beta2 and beta3. Calcif Tissue Int 2007; 80: 259-67
  9. Sawyer JR, Myers MA, Rosier RN, Puzas JE. Heterotopic ossification: clinical and cellular aspects. Calcif Tissue Int 1991; 49: 208-15
  10. Shen Q, Zhu S, Hu J, Geng N, Zou S. Recombinant human bone morphogenetic protein-4 (BMP-4)-stimulated cell differentiation and bone formation within the expanding calvarial suture in rats. J Craniofac Surg 2009; 20: 1561-5
  11. Sampath TK, Maliakal JC, Hauschka PV, et al. Recombinant human osteogenic protein-1 (hOP-1) induces new bone formation in vivo with a specific activity comparable with natural bovine osteogenic protein and stimulates osteoblast proliferation and differentiation in vitro. J Biol Chem 1992; 267: 20352-62
  12. Evans KN, Forsberg JA, Potter BK, et al. Inflammatory cytokine and chemokine expression is associated with heterotopic ossification in high-energy penetrating war injuries. J Orthop Trauma 2012; 26: e204-e13
  13. Rodenberg E, Azhdarinia A, Lazard ZW, et al. Matrix metal