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Journals / Adli Tıp Bülteni / 1996 / Cilt: 1 - Sayı: 3

Determination of acute and chronic exposure to carbon monoxide by spectrophotometric methods

Karbonmonoksit'e (CO) akut ve kronik maruziyetin spektrofotometrik yöntemlerle tayini

Nevin VURAL
Cem YÜCESOY
Kahraman Rukiye DOĞANYİĞİT
Pages
112–115
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Also it was shown that this method can be used easily in Forensic Medicine Laboratories as well as the procedure takes a short time.

📌 3 Key Takeaways

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Objective & Hypothesis:COHb levels in the blood samples of victims of suspec¬ted CO poisoning (n: 18) and subjects exposed to low CO (smokers n: 100, nonsmokers n: 44) were measured by spectrophotometric methods.
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Key Findings & Data:COHb levels of postmortem blood samples were found to be between 63.00 ( 88.00 % (mean 73-11 % ( 6.90).
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Conclusion & Impact:Also it was shown that this method can be used easily in Forensic Medicine Laboratories as well as the procedure takes a short time.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

COHb levels in the blood samples of victims of suspec¬ted CO poisoning (n: 18) and subjects exposed to low CO (smokers n: 100, nonsmokers n: 44) were measured by spectrophotometric methods. Derivative UV-spectrophotometric method was also used for the determination of COHb in the blood of smo¬kers. The sensitivity and reliability of these methods were compared. COHb levels of postmortem blood samples were found to be between 63.00 ( 88.00 % (mean 73-11 % ( 6.90). COHb levels of 1-9 cigarettes smokers daily (n=44) were found to be 1.94 ( 0.11 % COHb; 3-25 ( 0.19 % COHb in smokers (10-20 cigarettes daily, n=23). COHb level in all smokers (4.36 ( 0.14 %) were found significantly higher then the nonsmo¬kers (p<0.01, p<0.05). Dubowski's method for the quantati-ve estimation of the CO poisoning was evaluated as a sen¬sitive and economic method among the spectrophotometric methods. Also it was shown that this method can be used easily in Forensic Medicine Laboratories as well as the procedure takes a short time.

Özet

CO zehirlenmesi sonucu ölen kişilerin (n: 18) ve CO'e kronik maruz kalan kişilerin (sigara içenler n: 100, içmeyen¬ler n: 44) kanlarında karboksihemoglobin (COHb) düzeyle¬ri spektrofotometrik yöntemlerle ölçülmüştür. Postmortem kan örneklerinde COHb tayininde, Dubowski ve Buch-wald'in modifiye mikro spektrofotometrik yöntemlerinden yararlanılmıştır. Sigara içenlerin kan örneklerinde COHb ta¬yininde ise türev (derivative) UV spektrofotometresinclen yararlanılmış ve yöntemlerin karşılaştırılması yapılmıştır. Postmortem kan örneklerinde COHb düzeyi % 63 - 88 arasında (ortalama % 73.11 ( 6.90) bulunmuştur. Sigara içen¬lerde COHb düzeyleri ise, günde 1-9 tane içenlerde (n: 44) %' 1.94 ( 0.11 COHb, 10-20 tane içenlerde (n: 23) % 3.25( 0.19 COHb ve > 20 tane içenlerde ise (n: 3D % 4.36 ( 0.14 COHb belirlenmiştir. COHb düzeyi sigara içen tüm gruplar¬da içmeyenlere göre ( % 0.86 ( 0.05) anlamlı yüksek bulun¬muştur. Dubowski yöntemi, CO ile akut zehirlenmelerin kantita-tif tayininde, kullandığımız diğer spektrofotometrik yöntem¬lere göre ekonomik ve duyarlı bir yöntem olarak değerlen¬dirilmiştir. Ayrıca kısa zamanda sonuç vermesi açısından da Adli Tıp laboratuvarında uygulanabilirliği bu araştırmada gösterilmiştir.

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Cite This Article

VURAL, N., YÜCESOY, C., DOĞANYİĞİT, K. R. (1996). Determination of acute and chronic exposure to carbon monoxide by spectrophotometric methods. Adli Tıp Bülteni, Cilt: 1 - Sayı: 3, 112–115. https://ajindex.com/articles/353397

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References (2)

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