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Journals / International Journal of Medical Biochemistry / 2020 / Cilt: 3 - Sayı: 2

The most common preanalytic problem of sweat testing: Insufficient sweat volume

Ayşegül ÖZGENÇ
Nesibe Esra YAŞAR
Mine KUCUR
İbrahim Murat BOLAYIRLI
DİLDAR KONUKOĞLU
Pages
62–66
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Laboratory staff and parents should be well informed about preanalytical factors before a sweat analysis is performed in order to reduce the QNS rate.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: A sweat test is the gold standard method for the diagnosis of cystic fibrosis (CF).
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Key Findings & Data:The aim of this study was to determine rates of insufficient sweat volume and evaluate the relationship of sweat volume with demographic characteristics of patients, as well as the implementation of corrective preventive actions.Methods: This study was performed retrospectively in the sweat test laboratory of the Cerrahpasa Faculty of Medicine of Istanbul University-Cerrahpasa.
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Conclusion & Impact:Laboratory staff and parents should be well informed about preanalytical factors before a sweat analysis is performed in order to reduce the QNS rate.
🔬Design:Modeling & Simulation
📍Scope:2016 · 545 specimens
⚙️Variables:Domain Parameters

Özet

Objectives: A sweat test is the gold standard method for the diagnosis of cystic fibrosis (CF). The most important preanalytical error for sweat testing is insufficient sweat volume. The aim of this study was to determine rates of insufficient sweat volume and evaluate the relationship of sweat volume with demographic characteristics of patients, as well as the implementation of corrective preventive actions.Methods: This study was performed retrospectively in the sweat test laboratory of the Cerrahpasa Faculty of Medicine of Istanbul University-Cerrahpasa. A total of 545 specimens that were referred to the laboratory between May and December 2016 were evaluated. Sweating was stimulated with pilocarpine iontophoresis, and the Macroduct Coil System (Wescor, Inc., Logan, UT, USA) was used as the sweat collection system. Sweat volume 3 months (p=0.019). There was no significant difference in the QNS ratios according to gender (p=1.000) or the season when the test was applied (p=0.181).Conclusion: The determination of QNS rates is a crucial step in the standardization of the preanalytical conditions of a sweat test. In this study, the QNS rate was above that recommended by the Cystic Fibrosis Foundation ( 3 months). High QNS ratios may be due to the inadequacy of optimization of pre-test preparation. Laboratory staff and parents should be well informed about preanalytical factors before a sweat analysis is performed in order to reduce the QNS rate.

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Cite This Article

ÖZGENÇ, A., YAŞAR, N. E., KUCUR, M., BOLAYIRLI, İ. M., KONUKOĞLU, D. (2020). The most common preanalytic problem of sweat testing: Insufficient sweat volume. International Journal of Medical Biochemistry, Cilt: 3 - Sayı: 2, 62–66. https://ajindex.com/articles/347903

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References (13)

  1. Domingos MT, Magdalena NI, Cat MN, Watanabe AM, Rosário Filho NA. Sweat conductivity and coulometric quantitative test in neonatal cystic fibrosis screening. J Pediatr (Rio J) 2015;91(6):590–5
  2. Abdulhamid I, Kleyn M, Langbo C, Gregoire-Bottex M, Schuen J, Shanmugasundaram K, et al. Improving the Rate of Sufficient Sweat Collected in Infants Referred for Sweat Testing in Michigan. Glob Pediatr Health 2014;1:2333794X14553625
  3. Republic of Turkey, The Ministry of Health, Public Health Agency, Control program for hereditary metabolic diseases 2018-2021. Available at: https://hsgm.saglik.gov.tr/ depo/birimler/saglikli-beslenme-hareketli-hayat-db/Colyak/ Taslaklar/Kalitsal-Metabolizma-Hastaliklari-Kontrol-Programi-8-11-2017.pdf. Accessed Apr 7, 2020
  4. Beauchamp M, Grey V, Lands LC. Sweat collection for testing in Canadian Cystic Fibrosis Centers, is it optimal?. Clin Biochem 2005;38(10):934–7
  5. Davies JC, Alton EW, Bush A. Cystic fibrosis. BMJ 2007;335(7632):1255–9
  6. Castellani C, Southern KW, Brownlee K, Dankert Roelse J, Duff A, Farrell M, et al. European best practice guidelines for cystic fibrosis neonatal screening. J Cyst Fibros 2009;8(3):153–73
  7. AACB Sweat Testing Working Party, Coakley J, Scott S, Doery J, Greaves R, Talsma P, et al. Australian guidelines for the performance of the sweat test for the diagnosis of cystic fibrosis: report from the AACB Sweat Testing Working Party. Clin Biochem Rev 2006;27(2):S1–7
  8. Dikmen ZG, Pinar A, Akbiyik F. Specimen rejection in laboratory medicine: Necessary for patient safety?. Biochem Med (Zagreb) 2015;25(3):377–85
  9. Plebani M, Sciacovelli L, Aita A, Padoan A, Chiozza ML. Quality indicators to detect pre-analytical errors in laboratory testing. Clin Chim Acta 2014;432:44–8
  10. Farrell PM, Rosenstein BJ, White TB, Accurso FJ, Castellani C, Cutting GR, et al. Guidelines for diagnosis of cystic fibrosis in newborns through older adults: Cystic Fibrosis Foundation consensus report. J Pediatr 2008;153(2):S4–S14
  11. Dalcin Pde T, Abreu E Silva FA. Cystic fibrosis in adults: diagnostic and therapeutic aspects. J Bras Pneumol 2008;34(2):107–17
  12. LeGrys VA, Yankaskas JR, Quittell LM, Marshall BC, Mogayzel PJ Jr; Cystic Fibrosis Foundation. Diagnostic sweat testing: the Cystic Fibrosis Foundation guidelines. J Pediatr 2007;151(1):85–9
  13. Baumer JH. Evidence based guidelines for the performance of the sweat test for the investigation of cystic fibrosis in the UK. Arch Dis Child 2003;88(12):1126–7.