Journals / Kulak Burun Boğaz İhtisas Dergisi / 2006 / Cilt: 16 - Sayı: 5

A general review on the procedures in a human temporal bone laboratory

İnsan temporal kemik laboratuvarı prosedürleri:Genel bir bakış

Pages
235–240
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This article aims to provide insight into the functions of temporal bone laboratories and to familiarize the reader with histopathologic studies conducted therein.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Structures of the human ear are usually inaccessible during life for examination of pathologic changes of the underlying disease, which is only possible with postmortem studies of the human temporal bone.
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Key Findings & Data:Hence, human temporal bone laboratories serve as a unique source of material for research in this respect.
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Conclusion & Impact:This article aims to provide insight into the functions of temporal bone laboratories and to familiarize the reader with histopathologic studies conducted therein.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:İnsan · Diseksiyon · Temporal kemik

Abstract

Structures of the human ear are usually inaccessible during life for examination of pathologic changes of the underlying disease, which is only possible with postmortem studies of the human temporal bone. Hence, human temporal bone laboratories serve as a unique source of material for research in this respect. They enable comparison between histologic findings of temporal bone sections and the ear pathologies documented prior to death, as well as comparison of diseased ears with any selected temporal bone specimens, both of which provide invaluable knowledge to be shared among researchers and other laboratories. This article aims to provide insight into the functions of temporal bone laboratories and to familiarize the reader with histopathologic studies conducted therein.

Özet

İnsan kulağındaki yapılar genellikle yaşam boyunca "erişilemez" özellikte olup, altta yatan hastalıklara ait patolojik değişikliklerin incelenebilmesi postmortem çalışmalarla mümkün olabilmektedir. İnsan temporal kemik (İTK) laboratuvarları bu açıdan eşsiz bir materyal kaynağı sunarlar. Bu laboratuvarlar, temporal kemik kesitlerindeki histolojik bulgularla hastanın ölmeden önceki kulak patolojisinin karşılaştırılmasına, hastalıklı olguları diğer olgularla karşılaştırmaya ve elde edilen bilgilerin diğer laboratuvarlarla paylaşılmasına olanak verir. Bu makalede insan temporal kemik laboratuvarlarının işlevinin tanıtılması amaçlandı ve yapılan histopatolojik işlemlerin ayrıntılı analiziyle birlikte bu laboratuvarlar hakkında genel bir bakış açısı sunuldu.

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Cite This Article

OKTAY, M. F., CÜREOĞLU, S., SCHACHERN, P. A., PAPARELLA, M. M. (2006). A general review on the procedures in a human temporal bone laboratory. Kulak Burun Boğaz İhtisas Dergisi, Cilt: 16 - Sayı: 5, 235–240. https://ajindex.com/articles/343303

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References (15)

  1. Frost AR, Sparks D, Grizzle WE. Methods of antigen recovery vary in their usefulness in unmasking specific antigens in immunohistochemistry. Appl Immunohistochem Mol Morphol 2000;8:236-43
  2. Grizzle WE, Manne U, Jhala NC, Weiss HL. Molecular characterization of colorectal nedplasia in translational research. Arch Pathol Lab Med 2001;125:91-8
  3. Schuknecht HE Methods of removal, preparation and study. In: Schuknecht HF, editor. Pathology of the ear. 2nd ed. Philadelphia: Lea a n d Febiger; 1993. p. 1-27
  4. Keithley EM, Truong T, Chandronait B, Billings PB. Immunohistochemistry and microwave decalcification of human temporal bones. Hear Res 2000; 148:192-6
  5. Cunningham CD 3rd, Schulte BA, Bianchi LM, Weber PC, Schmiedt BN. Microwave decalcification of human temporal bones. Laryngoscope 2001;lll:278-82
  6. Schuknecht HE Light a n d electron microscopy on the same temporal bone. Ann Otol Rhinol Laryngol Suppl. 1989;143:40
  7. Ishiyama A, Lopez I, Wackym PA. Choline acetyltransferase immunoreactivity in the human vestibular end-organs. Cell Biol Int 1994;18:979-84
  8. Wackym PA. Molecular temporal bone pathology: I. Historical foundation. Laryngoscope 1997;107:1156-64
  9. Tian Q, Linthicum FH Jr, Keithley EM. Application of labeling techniques to archival temporal bone sections. Ann Otol Rhinol Laryngol 1999;108:47-53
  10. Shi SR, Cote RJ, Taylor CR. Antigen retrieval immunohistochemistry used for routinely processed celloidinembedded human temporal bone sections: standardization and development. Auris Nasus Larynx 1998; 25:425-43
  11. Shi SR, Cote RJ, Taylor CR. Antigen retrieval techniques: current perspectives. J Histochem Cytochem 2001;49:931-7
  12. Shi SR, Key ME, Kalra KL. Antigen retrieval in formalin- fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 1991;39:741-8
  13. Shi SR, Cote C, Kalra KL, Taylor CR, Tandon AK. A technique for retrieving antigens in formalin-fixed, routinely acid-decalcified, celloidin-embedded human temporal bone sections for immunohistochemistry. J Histochem Cytochem 1992;40:787-92
  14. Shi SR, Tian Q. Development of an antigen retrieval technique for immunohistochemistry on archival celloidin- embedded sections. J Histochem Cytochem 1993;41:1121
  15. Ganbo T, Sando I, Balaban CD, Suzuki C, Sudo M. Immunohistochemistry of lymphocytes and macrophages in human celloidin-embedded temporal bone sections ~ with acute