Journals / Kulak Burun Boğaz İhtisas Dergisi / 2014 / Cilt: 24 - Sayı: 1

Evaluation of shrinkage of surgical margins on tongue specimens following formalin fixation

Formalin fiksasyonu sonrası dil örneklerinde cerrahi sınır küçülmesinin değerlendirilmesi

Pages
30–37
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Therefore, 1.42 may be used as a corrective factor for sheep tongue tissues.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: This study aims to evaluate the alterations in distances to the surgical margins on sheep tongue specimens, through resection, formalin fixation, frozen section, and microscopic examination.
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Key Findings & Data:A total of 40 specimens with either 1 cm or 2 cm distances as surgical safety margins of the surrounding plate were prepared using either scalpel or monopolar cautery (10 specimens were prepared for each group).
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Conclusion & Impact:Therefore, 1.42 may be used as a corrective factor for sheep tongue tissues.
🔬Design:Research Article
📍Scope:40 specimens
⚙️Variables:Domain Parameters

Abstract

Objectives: This study aims to evaluate the alterations in distances to the surgical margins on sheep tongue specimens, through resection, formalin fixation, frozen section, and microscopic examination. Materials and Methods: Fifty fresh sheep tongues were used in the study. A metal plate was fixed on the lateral aspect of each tongue to represent tumor tissues. A total of 40 specimens with either 1 cm or 2 cm distances as surgical safety margins of the surrounding plate were prepared using either scalpel or monopolar cautery (10 specimens were prepared for each group). Additional 10 specimens with 1 cm safety margins were prepared using either scalpel or monopolar cautery (5 specimens per group) for frozen section examination. Distances to the metal plates before resection were compared with the ones which were determined after resection, frozen section examination, 24-hour formalin fixation and microscopic examination. Results: Distances to the surgical margins were found to be decreased in all specimens after resection, 10% formalin fixation and microscopic examination. The distances to the surgical margins were observed to be reduced by 6.5-7.5% on average after resection, 10-12% on average after formalin fixation and 30% on average after microscopic examination, compared to the baseline values. The level of shrinkage was reduced by 6.3-10% following microscopic section preparation during frozen section examination. Conclusion: Seven to eight-millimeter distance to the surgical margin at minimum should be maintained to achieve a 5 mm in height surgical safety margin during sheep tongue resection. The distance defined by the pathologist may be multiplied with 1.42 to estimate around in-situ distance to the surgical margins. Therefore, 1.42 may be used as a corrective factor for sheep tongue tissues.

Özet

Amaç: Bu çalışmada koyun dil örneklerinde cerrahi sınır mesafesinin rezeksiyon, formalin fiksasyonu, frozen inceleme ve mikroskobik inceleme ile deği- şimi değerlendirildi. Gereç ve Yöntem: Çalışmada 50 adet taze koyun dili kullanıldı. Dillerin lateral bölümüne tümör dokusunu temsilen metal plak yerleştirildi. Bu plak etrafında 1 cm ve 2 cm cerrahi güvenlik sınırı bırakılarak ve bistüri ve monopolar koter kullanılarak, toplam 40 örnek (her bir grup için 10 örnek) hazırlandı. Frozen inceleme için 1 cmlik sınırı olan bistüri ve monopolar koter kullanılarak elde edilen ilave 10 örnek (her bir grup için 5 örnek) daha hazırlandı. Bu örneklerin eksizyon sonrası, 24 saatlik formalin fiksasyonu ve mikroskobik inceleme sonrası belirlenen cerrahi sınıra olan uzaklıkları ölçüldü ve eksizyon öncesi ölçümler ile karşılaştırıldı. Bulgular: Rezeksiyon, %10luk formalin fiksasyonu ve mikroskobik kesit hazırlama sonrası tüm örneklerde cerrahi sınıra olan uzaklıkların azaldığı saptandı. Başlangıç değerlerine kıyasla, uzaklıkların rezeksiyon sonrası ortalama %6.5-7.5, formalin fiksasyonu sonrası ortalama %10-12 ve mikroskopik ölçüm sonrası ortalama %30 oranında azaldığı görüldü. Frozen incelemede ise mikroskobik kesit hazırlama sonrasında %6.3-10 arasında daha az büzüşme olduğu saptandı. Sonuç: Koyun dili rezeksiyonu sırasında 5 mmlik temiz bir cerrahi sınır elde etmek için en az 7-8 mm mesafe bırakılmalıdır. Patolog tarafından belirlenen cerrahi sınır mesafesi 1.42 ile çarpıla

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Cite This Article

GÖKOĞLU, Ö., POLAT, K., AYDIN, E., UYSAL, İ. Ö., DOĞAN, M., IŞIKSAÇAN, Ö. Ö. (2014). Evaluation of shrinkage of surgical margins on tongue specimens following formalin fixation. Kulak Burun Boğaz İhtisas Dergisi, Cilt: 24 - Sayı: 1, 30–37. https://ajindex.com/articles/342485

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