Journals / Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi / 2003 / Cilt: 14 - Sayı: 2

Identification of the species origin using polyacrilamide gel isoelectric focusing (PAGIF) technique

Polyakrilamid jel özoelektrik fokuslama tekniğinin (PAGIF) et türlerinin ayırımında kullanılması

Pages
102–106
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Generally, if the anatomical landmarks are eliminated, it is difficult or impossible to identify the animals species.A variety of methods exist for the determination of the nature of muscle proteins such as Electrophoresis, Isoelectricfocusing, Immunological and histological methods.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The growth of international trade of meat, chicken, game, birds and fish have increased the number of adulteration attemps.
📊
Key Findings & Data:The food quality control laboratory has got an important role in the protection of consumer to be able to determine the different proteins of animal origin and their eventual misrepresentation.Therefore, identification of animal species is the major concern for the food related laboratories.
💡
Conclusion & Impact:Generally, if the anatomical landmarks are eliminated, it is difficult or impossible to identify the animals species.A variety of methods exist for the determination of the nature of muscle proteins such as Electrophoresis, Isoelectricfocusing, Immunological and histological methods.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:et muayenesi · gıda analizleri · metodoloji

Abstract

The growth of international trade of meat, chicken, game, birds and fish have increased the number of adulteration attemps. The food quality control laboratory has got an important role in the protection of consumer to be able to determine the different proteins of animal origin and their eventual misrepresentation.Therefore, identification of animal species is the major concern for the food related laboratories. In certain circumstances, it is possible to detect the presence of proteins belonging to an animal species different from the original source of the meat as labelled on the package. On the other hand, it seems that the who sale suppliers, retailers and the other meat related industry branches are usually unable to identify precisely the animal species sent to the market. Generally, if the anatomical landmarks are eliminated, it is difficult or impossible to identify the animals species.A variety of methods exist for the determination of the nature of muscle proteins such as Electrophoresis, Isoelectricfocusing, Immunological and histological methods.

Özet

Uluslararası et, balık, kümes ve av hayvan ticaretinin her geçen gün daha da gelişmesine karşılık, yapılan hileler de önemli ölçüde artmıştır. Genelde türler karkas ve büyük parça etlerine göre identifıye edilebilmesine rağmen; toptancılar, perakendeciler ve et endüstrisi ile uğraşan diğer kuruluşlar satışa çıkardıkları bazı et ürünlerinin, hangi hayvan etinden yapıldığını açık bir şekilde belirleyetnemektedirler. Bu nedenle çeşitli hayvan etleri türlerinin identifikasyonu, gıda analiz laboratuarlarının en önemli konularından birisi olma niteliğini korumaktadır. Uygun bir analiz yöntemiyle farklı kaynaklardan gelen etlere ait proteinler incelenerek tür ayırımı yapılabilmekte ve bu yolla ambalaj etiketinde belirtilen bilgilerin doğruluk derecesi kontrol edilebilmektedir.Hayvansal orijinli, farklı proteinlerin tanınmasında ve tüketici haklarının korunmasında gıda analiz laboratuarları önemli bir role sahiptir. Kas proteinlerinin yapısını belirlemede elektro forezis, İzoelektrik fokuslama, immünolojik ve histolojik metodlar gibi çok çeşitli yöntemler kullanılmaktadır.

🔖

Cite This Article

EKİCİ, K., ALİŞARLI, M. (2003). Identification of the species origin using polyacrilamide gel isoelectric focusing (PAGIF) technique. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 14 - Sayı: 2, 102–106. https://ajindex.com/articles/339907

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (1)

  1. Andrews AT (1981): Electrophoresis. s 182, Clarendon Pres, Oxford. 2-Batmaz H. (1988): Sığırlarda Perikarditis ve Myokarditis Travmaticanm Ayırıcı Tanısında Serum Protein Elektroforezinin Önemi Üzerine Deneysel Araştırmalar II. U.Ü.Vet. Fak. Derg. 7 (1,2,3): 7-11. 3-Bauer F, Hofmann K (1987): Elektrophoretische Tierartbestimmung Steigerung der Empfindlichkeit durch Peroxidaseforbung Der Myoglobine. Fleischwirtsch 67(7): 861-967. 4-Bauer F, Hofmann K (1989): Elektrophorestische Tierarten Identifizierung bei erhitztem Fleisch und Fleischerzeugnissen. Fleischwirtsch. 69(3):419-422. 5-Decker R, Holpert D, Pott HH, Plein K (1985): Erfahrungen zum Einsatz der isoelektrischen Fokussierung-auf Polyacrylamide (PAGİF in der Fleischhygiene. Fleischwirtsch. 65 (7): 857-861. 6-Dincer B (1987): The Effects of Curing and Cooling on the Differentiation of Species Origin of Meat Procuts by Izoelectric Focusing. A.Ü. Vet. Fak. Derg. 34 (1): 97-104. 7-Dinçer B, Spearow JL, Cansens RG, Graerer ML (1987): The Effects of Curing on Cooking on The Detection of Species Origin of Meat Products by Competetive and Indirect ELİSA Techniques. Meat Science 20(4): 253-267. 8-Dobbelare S, Hithcock HS (1985): The Performance of an SDS-PAGE and an ELÎSA Method for the Quantitive Analysis of Soya Protein in Meat Products. J Food Sience and Agriculture 36: 499-507. 9-Droyer RH, Lata GF (1989): Electrophoresis in Experimental Biochemistry. ISBN 0-19-50508385 Oxfort University Press.Inc. New york. 10-Gissel CV (1987): Die Anwendung der isoelektrischen Focussierung an Polyacrylamidgelen (PAGİF) Zur Tierarten Differenzierung in Der Praxis. Archiv Fur Lebensmittelhygiene 38: 1-32. 11-HışıI Y (1994): Enstrumental Gıda Analizleri. E.Ü. Müh. Fak. Ders kitapları, s.162-165, Yayın No: 30, E.Ü.Basımevi, İzmir. 12-Hill CR (1991): SCI Biotechnology Group Meeting Analitical Methods in Biotechnology. J.Chem Tech. Biotechl. 51: 115-140. 13-Hitchcock CHS, Crimes AD(1985): Methodology for Meat Species Identification. Meat Science 15: 215-224. 14-Hoffman K, Penny IF (1985): Methoden zur Identifierung und Quantativer Bestimmung von Fleisch und Fremdeiweiss mit Hilfe der SDS-Polyacrilamide-Electrophores auf Flachgelen. Fleischwirtschaft 3 (2): 252-254. 15-Hoffman K, Bluctel E (1986): Bestimmung der Tierartz von rohem Muskelfleisch anhand der Myoglobinmuster im pH Gradienten Gel. Fleischwirtscahft, 66 (5): 916-921. 16-Iwabuchi S, Yamauchi F (1987): Electrophoretic Analysis of Whey Proteins Present in Soybean Gl