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Journals / Yeni Tıp Dergisi / 2014 / Cilt: 31 - Sayı: 3

Türk populasyonununda tip 2 diabet hastalarında insülin substrat-2 geni g1057d polimorfizmi

The G1057D polymorphism of insulin receptor substrate-2 (IRS2) gene with type 2 diabetes in the Turkish population

Sevim ÇELİK KARAKAŞ
Nurcan ATEŞ ARAS
MERAL URHAN KÜÇÜK
Serap YALIN
Esen AKBAY
Pages
168–171
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusions: These results strongly argue against a major role of the G1057D IRS-2 polymorphism in the pathogenesis of type 2 diabetes in Turkish subjects.

📌 3 Key Takeaways

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Objective & Hypothesis:Background: Insulin receptor substrate (IRS) proteins are critical to signal transduction in insulin target tissues.
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Key Findings & Data:IRS-2 knockout mice exhibit a phenotype similar to human type 2 diabetes, characterized by insulin resistance with abnormal glucose tolerance at birth culminating in the development of fasting hyperglycemia in later age.
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Conclusion & Impact:Conclusions: These results strongly argue against a major role of the G1057D IRS-2 polymorphism in the pathogenesis of type 2 diabetes in Turkish subjects.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background: Insulin receptor substrate (IRS) proteins are critical to signal transduction in insulin target tissues. IRS-2 knockout mice exhibit a phenotype similar to human type 2 diabetes, characterized by insulin resistance with abnormal glucose tolerance at birth culminating in the development of fasting hyperglycemia in later age. While several polymorphisms have been identified in the IRS-2 gene, the association of the Gly1057Asp polymorphism with type 2 diabetes has been studied in different populations, but the results have been inconsistent. The present study was undertaken to determine IRS-2 G1057D polymorphism association with Diabetes mellitus in Turkish subjects. Material and Methods: The study population consisted of 328 subjects 138 patients with DM and 190 controls. DNA was extracted from peripheral blood. Genetic polymorphism of IRS-2 G1057D was detected by using PCR-based restriction fragment-length polymorphism. Results: The genotype distribution of the IRS2 G1057D polymorphism was not statistically significant between groups. Conclusions: These results strongly argue against a major role of the G1057D IRS-2 polymorphism in the pathogenesis of type 2 diabetes in Turkish subjects.

Özet

Amaç: İnsülin reseptör substratı (IRS) proteini, hedef dokulara insülin sinyalinin iletilmesinde kritik bir öneme sahiptir. IRS-2 knockout farelerin insanlardaki tip 2 diabete benzer bir klinik fenotip gösterdiği tespit edilmiştir. IRS2 geninde çok sayıda polimorfizm belirlenmiş olmakla birlikte farklı populasyonlarda tip 2 diabet ile IRS-2 Gly1057Asp polimorfizmi arasındaki ilişki araştırılmış olup elde edilen sonuçlar oldukça çelişkilidir. Bu çalışmada Türk populasyonunda Diabetes mellitus ile IRS-2 G1057D polimorfizmi arasındaki ilişkiyi araştırmak amaçlanmıştır. Materyal ve Metot: Çalışma populasyonu 328 kişiden oluşmakta olup 138 kişi DM hastası ve 190 kişide kontroldür. DNA periferik kandan izole edilmiş olup IRS2 G1057D polimorfizmi PCR-RFLP yöntemi kullanılarak belirlenmiştir. Bulgular: IRS2 G1057D polimorfizmi genotip dağılımlarının gruplar arasında istatistiksel olarak anlamlı bir farklılık göstermediği tespit edilmiştir. Sonuçlar: Elde edilen bulgular IRS2 G1057D polimorfizminin tip 2 diabetin patogenezinde rolü olmadığını göstermektedir.

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Cite This Article

KARAKAŞ, S. Ç., ARAS, N. A., KÜÇÜK, M. U., YALIN, S., AKBAY, E. (2014). Türk populasyonununda tip 2 diabet hastalarında insülin substrat-2 geni g1057d polimorfizmi. Yeni Tıp Dergisi, Cilt: 31 - Sayı: 3, 168–171. https://ajindex.com/articles/338383

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