Journals / Van Tıp Dergisi / 2003 / Cilt: 10 - Sayı: 4

Biochemical characteristics and antibiotic susceptibilities of Vibrio metschnikovii strains isolated from various specimens

Çeşitli örneklerden izole edilen Vibrio metschnikovii suşlarının biyokimyasal özellikleri ve antibiyotiklere duyarlılıkları

Pages
101–107
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Data about this bacteria which we will likely meet more often is still restricted and it is necessary to make more comprehensive studies about this subject especially including water sources.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: the purpose of this study was to determine the biochemical characteristics and the antibiotic susceptibilities of V.metshnikovii strains yielded during a two year period between 1999-2001, front the specimens delivered to o.ur Microbiology Laboratory and the specimens received from various clinics for prevention and control of nosocomial infections.
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Key Findings & Data:Method: 137 specimens received from, various clinics were inoculated to bfoo'd ağar an eosine meihilene blue (EMB) agqr.
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Conclusion & Impact:Data about this bacteria which we will likely meet more often is still restricted and it is necessary to make more comprehensive studies about this subject especially including water sources.
🔬Design:Research Article
📍Scope:2001 · 137 specimens
⚙️Variables:İlaç direnci · mikrobiyal · Mikrobiyal duyarlılık testleri

Abstract

Aim: the purpose of this study was to determine the biochemical characteristics and the antibiotic susceptibilities of V.metshnikovii strains yielded during a two year period between 1999-2001, front the specimens delivered to o.ur Microbiology Laboratory and the specimens received from various clinics for prevention and control of nosocomial infections. Method: 137 specimens received from, various clinics were inoculated to bfoo'd ağar an eosine meihilene blue (EMB) agqr. Detection and antibiotic susceptibility tests of the yielded colonies were performed using "Sceptor Gram negative Enteric MIC MIC/ID panel" and "Sceptor Gram negative urine MIC/IP Panel (Bccton. Dickinson-USA). Results: From the cultures of the collected specimens, V.metshnikovii strains were isolated from a total of 23 specimens: 14. (61 %) were from the clinical specimens sent to. our laboratory and 9 (39 %) were from the specimens received from- Wipus clinics for control. In the result of susceptibility tests against antimicrobial agents, the most effective agents and their susceptibility rates were as follows: imipenem and amoxicillin-clavulanic acid 100 %, ampicillin-sulbactam, gentamicin and amikacin 95 %, tetracycline 92 %, cefazolin and ticarcuine-clavulanic acid 90 %. The highest resistance rates were detected against azfreonam with 80 %, ceftazidime with 60 %, cèfotaxime with 52 % and trimethoprim-sulphamethoxàzole with 48 %. V.metshnikovii strains demonstrated a resistance'rate between 13 % and 45 % against ampiçiİlin, cefoperäzone, cefptetah, ceftriaxpne, céfùroxime, ciprofloxacin, piperaciilin, ticarciHin and tobramycin. Conclusion: V.metschnikovii is considered to colonize for a period in various equipments in our hospital. Data about this bacteria which we will likely meet more often is still restricted and it is necessary to make more comprehensive studies about this subject especially including water sources.

Özet

Amaç: Çalışmada, 1999-2001 tarihleri arasındaki iki yıllık süreçte Mikrobiyoloji Laboratııvan'na gönderilen örnekler ile hastane enfeksiyonlarının önlenmesi ve kontrolü amacıyla çeşitli servislerden alınan örneklerden üretilen Vibrio ınetschnlkovii (F. metschnikovii) suşlarının biyokimyasal özellikleri ile antibiyotik hassasiyet testi sonuçları irdelenmiştir. Yöntem: Kliniklerden gönderilen 137 örnek, Kanlı ve Eozin Metilen Blue (EMB) Ağar besiyerlerine ekildikten sonra, kolonilerin tanımlanması ve antibiyotik duyarlılık testlerinde "Sceptor Gram Negative Enterik MIC/ID Panel" ile "Sceptor Gram Negative Urine MIC/ID Paneller" (Becton Dickinson-USA) kullanıldı. Bulgular: Laboratuvara gönderilen klinik örneklerinl4 (% 61)'ünde vè kontrol amaçlı olarak çeşitli servislerden alınan örneklerin 9(%39)'ıından olmak üzere toplam 23 örnekte V. metschnikovii izole edilmiştir. Antimikrobiyal ajanlara karşı yapılan duyarlılık testi sonucunda imipenem ve amoksisilin-klavulanatın %100, ampisilin-sulbaktam, gehtamisin ve amikasinin % 95, tetrasiklinin % 92, sefazolin ve tikarsilin-klavulanatın % 90 oranları ile en etkili ajanlar oldukları gözlenmiş; en yüksek direnç gelişimi saptanan ajanlar ise % 8.0 oranı ile aztreonam, % 60 oranı ile seftazidinı, % 52 oranı ile sefotaksim ve % 48 oranı ile trimetpprim-sulfametoksazol olarak saptanmıştır. V. metschnikovii suşlarının ampisilin, sefoperazon, sefotetan, seftriakson, sefuroksinı, siprofloksasin, piperasilin, tikarsilin ve tobramisine karşı % 13 ile % 45 arasında değişen oranlarda direnç geliştirdikleri tespit edilmiştir. Sonuç: V. metschnikovii'nin bir dönem hastanemiz bünyesindeki bulunan çeşitli cihaz ve ortamlarda kolonize olduğu gözlenmektedir ve çalışmada buna dikkat çekilmiştir. Giderek daha sık olarak karşımıza çıkan bu bakteri ile ilgili veriler yine de sınırlıdır ve özellikle su kaynakları başta olmak üzere, ülkemizde konu ile ilgili daha geniş çalışmalar yapılması gerekmektedir

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Cite This Article

GÜDÜCÜOĞLU, H., BOZKURT, H., KURTOĞLU, M. G., BAYRAM, Y., YAMAN, G., BERKTAŞ, M. (2003). Biochemical characteristics and antibiotic susceptibilities of Vibrio metschnikovii strains isolated from various specimens. Van Tıp Dergisi, Cilt: 10 - Sayı: 4, 101–107. https://ajindex.com/articles/334618

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