Journals / Van Tıp Dergisi / 2007 / Cilt: 14 - Sayı: 2

Investigation of resistance rates to methicillin with nasal staphylococcus aureus carrige at staff of Y.Y.Ü. medical faculty research hosbital

Y.Y.Ü.Tıp Fakültesi Araştırma Hastanesi personelinde nazal Staphylococcus aureus taşıyıcılığı ile metisiline direnç oranlarının araştırılması

Pages
52–56
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

This rate level was considered as a risk factor for nosocomial infections.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, Staphylococcus aureus which is the second cause of nosocomial infections were investigated for nasal carriage rates in our hospital staff and antimicrobial susceptibilities for meticillin and other several antibiotics were studied From 326 personnel’s (nurse, doctor, nurse’s aide, health technician, kitchen staff) working in Yuzuncu Yil University hospital, nose front cavity swab samples were taken using cotton swabs soaked into serum physiologic.
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Key Findings & Data:Single colony inoculation were performed on 5% sheep blood agar (SBA).l (nurse, doctor, nurses aide, technician, kitchen workers) with cotton swabs that were by sterile serum physiologic.
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Conclusion & Impact:This rate level was considered as a risk factor for nosocomial infections.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Sağlık personeli · İlaç direnci · bakteriyel

Abstract

In this study, Staphylococcus aureus which is the second cause of nosocomial infections were investigated for nasal carriage rates in our hospital staff and antimicrobial susceptibilities for meticillin and other several antibiotics were studied From 326 personnel’s (nurse, doctor, nurse’s aide, health technician, kitchen staff) working in Yuzuncu Yil University hospital, nose front cavity swab samples were taken using cotton swabs soaked into serum physiologic. Single colony inoculation were performed on 5% sheep blood agar (SBA).l (nurse, doctor, nurses aide, technician, kitchen workers) with cotton swabs that were by sterile serum physiologic. The samples were incubated at 37oC for 24 hours. After this time of incubation, the colonies which were like staphylococci colonies were stained by Gram staining. Catalase and coagulase tests were performed on strains which showed Gram positive coccus morphology. S. aureus strains were determined and straightened with Sceptor (Becton Dickinson - USA) system. Antimicrobial susceptibilities of all S.aureus strains were evaluated by disc diffusion method for oxacillin and several other antibiotics (erythromycin, teicoplanin, vancomycin, gentamicin, ciprofloxacin, clindamycin and mupirocin). Nasal carriage rate of S. aureus, in our hospital was found as 20.8% (19.7% doctors, 15.7% nurses, 25.3% nurse’s aides, 26.9% health technicians, 22.7% kitchen staff) and MRSA rate was 5.9%. This rate level was considered as a risk factor for nosocomial infections.

Özet

Bu çalışmada, hastane infeksiyonu etkenleri arasında en sık ikinci etken olan Staphylococcus aureus’un hastanemiz personelindeki nazal kolonizasyon oranları ile metisilin ve diğer bazı antimikrobiyallere karşı duyarlılıklarının araştırılması amaçlandı. Yüzüncü Yıl Üniversitesi Araştırma ve Uygulama Hastanesi’nde çalışan 326 personelin (hemşire, doktor, hastabakıcı, yardımcı sağlık teknisyeni, mutfak çalışanı) burun ön deliklerinden steril serum fizyolojik ile ıslatılmış eküvyon kullanılarak sürüntüler alındı. Yüzde 5 koyun kanlı agar plaklarına tek koloni ekimi yapıldı. Örnekler 37oC’de 24 saat inkübe edildi. Bu sürenin sonunda stafilokok kolonisine benzeyen koloniler Gram boyası ile boyandı. Gram pozitif kok morfolojisi gösteren kolonilere katalaz ve koagülaz testleri uygulandı. Sonuçlar Sceptor (Becton Dickinson - USA) cihazıyla da doğrulandı. Tüm S.aureus izolatlarının oksasilin ve diğer bazı antimikrobiyallere (eritromisin, teikoplanin, vankomisin, gentamisin, siprofloksasin, klindamisin ve mupirosin) duyarlılıkları disk difüzyon yöntemi ile araştırıldı. Burunda S. aureus taşıyıcılık oranı, hastanemizde %20.8 (doktorlarda %19.7, hemşirelerde 15.7, hastabakıcılarda %25.3, yardımcı sağlık teknisyenlerinde %26.9, mutfak çalışanlarında %22.7); MRSA oranı ise %5.9 olarak bulundu. Bu oran nozokomiyal infeksiyonlar için bir risk faktörü olarak değerlendirildi.

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Cite This Article

BOZKURT, H., BAYRAM, Y., GÜCÜOĞLU, H., BERKTAŞ, M. (2007). Investigation of resistance rates to methicillin with nasal staphylococcus aureus carrige at staff of Y.Y.Ü. medical faculty research hosbital. Van Tıp Dergisi, Cilt: 14 - Sayı: 2, 52–56. https://ajindex.com/articles/334201

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