AI Summary & Key Takeaways
AI InsightConclusion: It has been determined that quercetin has protective effect and decrease the injury occurred in experimental Lower Extremity Ischemia-Reperfusion Injury in Rats.
📌 3 Key Takeaways
Abstract
Objective: It was aimed to determine the protective effectiveness of quercetin in preventing lower extremity ischemia-reperfusion (I/R) injury. Material and Methods: 30 Sprague-Dawley male rats weighing 250-350 g were divided into three groups. Anaesthesia was applied to the first group of rats without ischemia-reperfusion process. To the second group two hours of ischemia and two hours of reperfusion, with the help of left lower extremities tourniquet , were applied. To the third group, 50mg/kg (ip) quercetin application was performed 45 minutes before the ischemia was initiated, two hours of ischemia and two hours of reperfusion application were carried out. At the end of the process, by taking the tissue samples, total antioxidant status (TAS) and total oxidant status (TOS) levels were evaluated. Results:When TAS was evaluated, it was determined that the decrease (p?0.001) occurring in I/R group in comparison with the control group was significant, the decrease (p?0.05) in I/R+Q group in comparison with control group, and again the increase (p?0.001) in I/R+Q group in comparison with I/R group was also significant. When the TOS amounts were evaluated, it was determined that the increase (p?0.001) occurring in I/R group in comparison with control group, the increase in I/R+Q group in comparison with control group (p?0.05), the decrease in I/R+Q group in comparison with I/R group was statistically significant. Conclusion: It has been determined that quercetin has protective effect and decrease the injury occurred in experimental Lower Extremity Ischemia-Reperfusion Injury in Rats.
Özet
Amaç: Alt ekstremite iskemi reperfüzyon (I/R) hasarını önlemede Quercetin'in koruyucu etkisinin belirlenmesi amaçlandı. Gereç ve Yöntem: Çalışmada 30 adet 250-350 gr ağırlığında Sprague- Dawley cinsi erkek sıçanlar üç gruba ayrıldı. Birinci gruptaki sıçanlara iskemi-reperfüzyon işlemi yapılmaksızın anestezi işlemi, ikinci gruptaki sıçanların sol alt ekstremitelerine turnike yardımıyla 2 saat iskemi, 2 saat reperfüzyon, üçüncü gruba ise iskemi başlatılmadan 45 dk. önce 50 mg/kg (ip) quercetin uygulamasının ardından 2 saat iskemi, 2 saat reperfüzyon uygulaması yapıldı. Süre sonunda sıçanlardan doku örnekleri alınarak, total antioksidan kapasite (TAS) ve total oksidan statüsü (TOS) düzeyleri değerlendirildi. Bulgular: TAS değerlendirildiğinde; I/R grubunda kontrol grubuna oranla meydana gelen azalışın (p?0.001) anlamlı, kontrol grubuna oranla I/R+Q grubundaki azalışın (p?0.05), yine I/R grubuna oranla, I/R+Q grubunda meydana gelen artışın (p?0.001) istatistiksel olarak anlamlı olduğu belirlendi. TOS miktarları değerlendirildiğinde I/R grubunda kontrol grubuna oranla meydana gelen artışın (p?0.001), kontrol grubuna oranla I/R+Q grubundaki artışın (p?0.05), I/R grubuna oranla, I/R+Q grubunda meydana gelen azalışın (p?0.001) istatistiksel olarak anlamlı olduğu belirlendi. Sonuç: Sıçanlarda deneysel Alt Ekstremite İskemiReperfüzyon Hasarında Quercetinin koruyucu etkisi olduğu ve meydana gelen hasarı azalttığı belirlendi.