Journals / Veteriner Bilimleri Dergisi . Eurasian Journal of Veterinary Sciences / 1998 / Cilt: 14 - Sayı: 2

Virus isolation from semen of bulls that using artificial and natural insemination

Suni ve tabii tohumlamada kullanılan boğaların spermalarından virus izolasyonu

Pages
39–46
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result of inoculation of 60 semen samples onto cell cultures, 5 viruses were isolated. 3 of the isolated viruses were identified as Bovine Herpesvirus Type 1 (BHV-1) according to the results of cross microneutralisation test by rabbit hyperimmun sera.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study; 60 semen samples from the artificial insemination centre in Ankara and also from the bulls -under the possessions of farmers- brought to be slaughtered in Meat and Fish Foundation Slaughterhouse in Konya were examined for virus isolation on sensitive cell cultures.
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Key Findings & Data:In this study; 60 semen samples from the artificial insemination centre in Ankara and also from the bulls -under the possessions of farmers- brought to be slaughtered in Meat and Fish Foundation Slaughterhouse in Konya were examined for virus isolation on sensitive cell cultures.
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Conclusion & Impact:As a result of inoculation of 60 semen samples onto cell cultures, 5 viruses were isolated. 3 of the isolated viruses were identified as Bovine Herpesvirus Type 1 (BHV-1) according to the results of cross microneutralisation test by rabbit hyperimmun sera.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:sığır herpes virüsü 1 · boğa · izolasyon

Abstract

In this study; 60 semen samples from the artificial insemination centre in Ankara and also from the bulls -under the possessions of farmers- brought to be slaughtered in Meat and Fish Foundation Slaughterhouse in Konya were examined for virus isolation on sensitive cell cultures. As a result of inoculation of 60 semen samples onto cell cultures, 5 viruses were isolated. 3 of the isolated viruses were identified as Bovine Herpesvirus Type 1 (BHV-1) according to the results of cross microneutralisation test by rabbit hyperimmun sera.

Özet

Bu araştırmada; Ankara'da bulunan suni tohumlama istasyonundan ve ayrıca halkın elinde bulunan, Konya Et ve Balık Kurumu Mezbahasında kesime getirilen, aşımda kullanılmış boğalardan alınan 60 sperma örneğinden duyarlı hücre kültürlerinde virus izolasyonu çalışmaları yapılmıştır. İncelenen 60 sperma örneğinin hücre kültürlerine inokulasyonu sonucu 5 adet virus izole edilmiştir, izole edilen virusların 3 tanesi, tavşanlardan hazırlanan hiperimmun serumla yapılan çapraz mikronötralizasyon testi sonuçlarına göre Bovine Herpesvirus tip-1 (BHV-1) olarak identifiye edilmiştir.

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Cite This Article

YAVRU, S., ÖZTÜRK, F., ŞİMŞEK, A., YAPKIÇ, O., YILDIZ, C. (1998). Virus isolation from semen of bulls that using artificial and natural insemination. Veteriner Bilimleri Dergisi . Eurasian Journal of Veterinary Sciences, Cilt: 14 - Sayı: 2, 39–46. https://ajindex.com/articles/333550

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