AI Summary & Key Takeaways
AI InsightThe β-lactamase activity of the strains was determined to have an important role in resistance against β-lactam group antibiotics.
📌 3 Key Takeaways
Abstract
This study was carried out in 256 cows which were in lactation period in Sarıkamış town of Kars. The animals were examined clinically in the point of mastitis and inflamational changes of udders were determined with California Mastitis Test (CMT). Consequently, mastitis were diagnosed in 61 of 256 cows (23.8%). 79 milk sample were taken from 61 cows which were positive in CMT test. As a result of microbiological examination, while agent was isolated in 67 of all samples (84.8%), no bacterial agent was not isolated in 12 samples (15.2%). While Staphylococcus aureus was the most determined bacterium with the percentage of 34.3%, coagulase negative staphylococcus (KNS) followed that whit the persentage of 28.3%. Except these two groups of microorganism, Escherichia coli was isolated in the 5.9% and Streptococcus spp. were isolated in the 16.4% of the samples. In 14 (33.3%) S. aureus and 4 ( 9.5% ) KNS strains, of all isolated staphylococcus strains, β-lactamase was found positive. In the study the resistance of staphylococcus strains against antibiotics were determined as follows; 59.5% penicillin, 38.1% amoxicillin, 7.1% amoxicillin-clavulonic acid, 4.8% cloxacillin, 11.9% enrofloxacin and all strains were found sensitive against vancomicin. The β-lactamase activity of the strains was determined to have an important role in resistance against β-lactam group antibiotics.
Özet
Bu çalışma Kars’ın Sarıkamış ilçesinde, laktasyon periyodunda olan 256 inek üzerinde yürütüldü. Hayvanlar mastitis yönünden klinik muayeneye tabi tutuldu ve memedeki yangısal değişikliler California Mastitis Testi (CMT) ile belirlendi. Sonuçta, 256 ineğin 61 (%23.8)’inin mastitisli olduğu saptandı. CMT pozitif olduğu belirlenen 61 inekten bakteriyolojik inceleme için 79 süt örneği alındı. Mikrobiyolojik inceleme sonucunda bu örneklerin 67 (%84.8)’sinde etken izole edilirken, 12 (%15.2)’sinde herhangi bir bakteriyel etken izolasyonu yapılamadı. Staphylococcus aureus, %34.3 oranı ile en sık saptanan bakteri olurken, bunu %28.3 oranı ile koagülaz negatif stafilokoklar (KNS) izledi. Bu iki grup mikroorganizma dışında örneklerin %16.4’ünde Streptococcus spp. ve %5.9’unda Escherchia coli izole edildi. İzole edilen stafilokok suşlarından 14 (%33.3) S.aureus ve 4 (%9.5) KNS suşun β-laktamaz pozitif olduğu bulundu. Stafilokok suşlarının antibiyotik dirençlilikleri; %59.5’i penisiline, %38.1’i amoksisiline, %7.1’i amoksisilin-klavulonik aside, %4.8’i kloksasiline, %11.9’u enrofloksasine dirençli ve suşların tamamının vankomisine duyarlı belirlendi. Suşların β-laktamaz aktivitesinin β- laktam grubu antibiyotiklere karşı dirençte önemli bir rol oynadığı tespit edildi.