Journals / Veteriner Hekimler Derneği Dergisi / 2019 / Cilt: 90 - Sayı: 2

Investigation of antibiotic susceptibility of Salmonella spp. strains’ isolated from raw chicken meat

Çiğ tavuk etlerinden izole edilen Salmonella spp. suşlarının antibiyotik duyarlılıklarının araştırılması

Pages
105–114
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

As a result of the antibiotic susceptibilities tests, according to the results of Vitek 2 Compact AST GN 38, all isolates were found to be resistant to amikacin, cephalexin, tobramycin, gentamycin and enrofloxacin. 29 (82.85%) isolates were found to be resistant to tetracycline and nitrofurantoin. 20 (57.15%) and 21 (60%) isolates were found to be resistant to ampicillin and piperacillin respectively.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to investigate the antibiotic susceptibility, resistance status and to determine the ESBL properties with Vitek in Salmonella spp. strains, which was isolated and identified from raw chicken meats.
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Key Findings & Data:As a result of isolation and identification, a total of 35 Salmonella spp. isolated.
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Conclusion & Impact:As a result of the antibiotic susceptibilities tests, according to the results of Vitek 2 Compact AST GN 38, all isolates were found to be resistant to amikacin, cephalexin, tobramycin, gentamycin and enrofloxacin. 29 (82.85%) isolates were found to be resistant to tetracycline and nitrofurantoin. 20 (57.15%) and 21 (60%) isolates were found to be resistant to ampicillin and piperacillin respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Antibiyotik direnç · Salmonella · tavuk eti

Abstract

The aim of this study was to investigate the antibiotic susceptibility, resistance status and to determine the ESBL properties with Vitek in Salmonella spp. strains, which was isolated and identified from raw chicken meats. In this study, chicken meats were analyzed for Salmonella spp. isolation. As a result of isolation and identification, a total of 35 Salmonella spp. isolated. As a result of the antibiotic susceptibilities tests, according to the results of Vitek 2 Compact AST GN 38, all isolates were found to be resistant to amikacin, cephalexin, tobramycin, gentamycin and enrofloxacin. 29 (82.85%) isolates were found to be resistant to tetracycline and nitrofurantoin. 20 (57.15%) and 21 (60%) isolates were found to be resistant to ampicillin and piperacillin respectively. In addition, extended spectrum beta-lactamase was detected in 4 isolates. According to the results of disc diffusion method, all isolates were found to be resistant against erythromycin. 34 (97.14%) of all isolates were found to be resistant against sulfonamide compounds, 28 isolates (80%) were found to be resistant against ampicillin sulbactam and sulfamethoxazole/trimethoprim, 26 isolates (74.28%) were found to be resistant against neomycin, 29 (82.85%) isolates were found to be resistant against oxytetracycline and tetracycline, 31 (88.57%), 17 (48.57%), 10 (28.57%), 6 (17.14%) were found to be resistant against ofloxacin, florfenicol, amoxicillin, chloramphenicol respectively. 24 (68.57%) and 6 (17.14%) isolates were found to be intermediate (I-drug increased contact sensitive) and resistant to ciprofloxacin, respectively. In view of these results, it was thought that, in terms of public health, it could cause infection in people consuming contaminated foods with multiple antibiotic resistance strains, antibiotics could not be effected due to multiple antibiotic resistance and increase in the number of resistant strains. Finally, it was thought that the studies on antibiotic resistance could be beneficial in terms of epidemiology

Özet

Bu çalışmada çiğ tavuk etlerinden izole edilen Salmonella spp. izolatlarının antibiyotik duyarlılık ve direnç durumlarının araştırılması, Vitek ile GSBL özelliklerinin belirlenmesi amaçlandı. Çalışmada, gıda olarak paketli satışa sunulan tavuk etleri Salmonella spp. izolasyonu amacıyla analiz edildi. İzolasyon ve identifikasyon sonucunda toplam 35 Salmonella spp. izole edildi. Antibiyotik duyarlılıklarının belirlenmesi amacıyla yapılan testlerin sonucunda, Vitek 2 Compact AST GN 38 kartı sonuçlarına göre amikasin, sefaleksin, tobramisin, gentamisin ve enrofloksasine ise tüm suşlar dirençli bulunurken, amoksisilin-klavulanik asite karşı tüm suşlar duyarlı bulundu. Tetrasiklin ve nitrofurantoine 29 (%82.85), ampisiline 20 (%57.15), piperasiline ise 21 (%60) izolat dirençli bulundu. Ayrıca 4 izolatta geniş spektrumlu beta laktamaz tespit edildi. Disk difüzyon yöntemi sonuçlarına göre ise, eritromisine karşı tüm izolatlar dirençli bulundu. Sülfonamid bileşiklerine karşı 34 (%97.14), ampisilin sulbaktam ve sülfametoksazol/ trimetoprime 28 (%80), neomisine 26 (%74.28), oksitetrasiklin ve tetrasikline 29 (%82.85), ofloksasine 31 (%88.57), florfenikole 17 (%48.57), amoksisiline 10 (%28.57), kloramfenikole 6 (%17.14) izolat dirençli bulundu. Siprofloksasine karşı 24 (%68.57) izolat orta (I) ve 6 (%17.14) izolat dirençli bulundu. Doksisikline karşı ise 18 (%51.42) izolat dirençli ve 9 (%25.71) izolat orta (I) bulundu. Enrofloksasine karşı ise 2 (%5.71) izolat dirençli ve 12 (%34.28) izolat orta (intermediate-I-ilaçla artmış temasta duyarlı) bulundu. Neomisine karşı ise 6 (%17.14) suş orta bulundu. Bu sonuçlar göz önüne alındığında, halk sağlığı açısından, çoklu antibiyotik direnci gösteren bu suşlarla kontamine gıdaları tüketen insanlarda infeksiyona neden olabileceği, antibiyotiklerin etki göstermeyebileceği ve dirençli suş sayısında artışların olabileceği ve yayılabileceği düşünüldü. Son olarak, antibiyotik direnci konusunda yapılacak çalışmaların epidemiyolojik açıdan yararlı olabileceği düşünüldü.

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Cite This Article

BABACAN, O., KARADENİZ, H. (2019). Investigation of antibiotic susceptibility of Salmonella spp. strains’ isolated from raw chicken meat. Veteriner Hekimler Derneği Dergisi, Cilt: 90 - Sayı: 2, 105–114. https://ajindex.com/articles/332212

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