SavedAdd your journal

Journals / Türk Tarım - Gıda Bilim ve Teknoloji dergisi / 2014 / Cilt: 2 - Sayı: 4

Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants

Yabani Baklagil Bitkilerinden, Mikrobiyal Gübre Olarak Kullanılan Rhizobium Spp. Bakterilerinin İzolasyonu

HATİCE ÖĞÜTCÜ
Ömer Faruk ALGUR
Pages
181–184
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The cytological and biochemical analysis of results showed that 28 of 39 strains belonged to Rhizobium spp.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In our study used wild leguminous plants (Medicago sativa, M. lupulina and M. varia ) Erzurum belongs to the high altitude (2000 - 2500m ) regions ( Palandöken mountain, Alibaba mountain, Turnagöl mountain, Hasanbaba mountain, Eğerli mountain, Yıldırım mountain, Çubuklu mountain, Deveboynu locality, Kayakyolu locality, Telsizler and Dumlu hill ) were collected during the months of June and July.
📊
Key Findings & Data:Nodules were obtained from this plant were sterilized, YMA (Yeast Mannitol Agar) plates were streaked and petri dishes 28+1ºC were incubated for 3-5 days.
💡
Conclusion & Impact:The cytological and biochemical analysis of results showed that 28 of 39 strains belonged to Rhizobium spp.
🔬Design:Research Article
📍Scope:2000 Verileri
⚙️Variables:Domain Parameters

Abstract

In our study used wild leguminous plants (Medicago sativa, M. lupulina and M. varia ) Erzurum belongs to the high altitude (2000 - 2500m ) regions ( Palandöken mountain, Alibaba mountain, Turnagöl mountain, Hasanbaba mountain, Eğerli mountain, Yıldırım mountain, Çubuklu mountain, Deveboynu locality, Kayakyolu locality, Telsizler and Dumlu hill ) were collected during the months of June and July. Nodules were obtained from this plant were sterilized, YMA (Yeast Mannitol Agar) plates were streaked and petri dishes 28+1ºC were incubated for 3-5 days. Colonies appear after incubation typically constitute (white, clear or slightly opaque, mucosity, round, raised) 39 isolates were selected and transferred to tubes and refrigerated YMA were stored at +4ºC. In the next stage, cytological and biochemical analyzes of these isolates were studied to determine. For this purpose isolates; YMA containing bromothymol blue and congo red reproduction, gram stain reaction, movement and subjected to catalase and oxidase tests were evaluated. The cytological and biochemical analysis of results showed that 28 of 39 strains belonged to Rhizobium spp.

Özet

Araştırmamızda kullanılan yabani baklagil bitkileri ( Medicago sativa, M.lupulina ve M. varia) Erzurum iline ait yüksek rakımlı (2000-2500m) bölgelerden (Palandöken dağı, Alibaba dağı, Turnagöl dağı, Hasanbaba dağı, Eğerli dağı, Yıldırım dağı, Çubuklu dağı, Deveboynu mevkii, Kayakyolu mevkii, Telsizler tepesi ve Dumlu tepesi) Haziran ve Temmuz aylarında toplandı. Bu bitkilerden elde edilen nodüller steril edilerek YMA (Yeast Mannitol Agar) plaklarına çizgi ekim yapıldı ve petriler 28+1 ºC’de 3-5 gün süreyle inkübasyona bırakıldı. İnkübasyon süresi sonunda oluşan tipik koloniler oluşturan (beyaz, saydam veya hafif mat, mukozlu, yuvarlak, kabarık) 39 izolat seçilerek yatık YMA tüplerine aktarıldı ve buzdolabında +4ºC’de muhafaza edildi. Sonraki aşamada bu izolatlar sitolojik ve biyokimyasal analizlerle tanımlanmaya çalışıldı. Bu amaçla izolatlar; Brom Thymol Mavili ve Kongo Kırmızılı YMA’da üreme, gram özelliği, hareket, katalaz ve oksidaz testlerine tabi tutularak değerlendirildi. Yapılan sitolojik ve biyokimyasal analizler sonucunda elde edilen 39 izolatın 28’inin Rhizobium spp. olduğu tespit edildi.

🔖

Cite This Article

ÖĞÜTCÜ, H., ALGUR, Ö. F. (2014). Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, Cilt: 2 - Sayı: 4, 181–184. https://ajindex.com/articles/331757

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Adıguzel A, Öğütcü H, Baris O, Karadayi M, Gulluce M, Sahin, F. 2010. Genetic Diversity of Rhizobium Strains Isolated from Wild Vetch Collected from High Altitudes in ErzurumTurkey. Romanian Biotechnological Letters., 15: 5017-5024
  2. Andeden EE, Baloch FS, Derya M, Kilian B, Özkan H. 2013. İPBS-Retrotransposons-based Genetic Diversity and Relationship Among Wild Annual Cicer Species. J. Plant Biochem. Biotechnol., 22: 453-466
  3. Atıcı Ö, Öğütcü H, Algur ÖF. 2005. Effect of Putrescine on İnducing Symbiosis in Chickpea and Vetch İnoculated with Standard or Wild Strains of Rhizobium. Symbiosis., 38: 163-174
  4. Beck DP, Materon LA, Afandi F. 1993. Practical Rhizobium – Legume Technology Manual. International Center for Agricultural Research in the Dry Areas (ICARDA). Aleppo Syria: P 1 – 54 Boşgelmez A, Boşgelmez İİ, Paslı N, Savaşçı S. 2001.
  5. EkolojiII., Ankara: 886s Gök M, Martin P. 1993. Farklı Rhizobium Bakterileri ile Aşılamanın Soya, Üçgül ve Fiğde Simbiyotik Azot Fiksasyonuna Etkisi. Doğa Tr. J. of Agricultural and Forestry., 17: 753-761
  6. Holt JG, Krieg NR, Sneath Ha A, Staley JT, Williams ST. 1994. Bergey’s Manual of Determinative Bacteriology, Williams & Wilkins, Baltimore, Maryland (9 nd ed ): p 5–98 Lajudie PD, Willems A, Pot B, Dewettinck GM, Neyra M, Collins MD, Dreyfus B, Kersters K, Gillis M. 1994. Polyphasic taxonomy of Rhizobia: Emendation of the genus Sinorhizobium and description of Sinorhizobium meliloti comb. nov., Sinorhizobium saheli sp.nov., and Sinorhizobium teranga sp.nov. International Journal Systematic Bacteriology.,44: 715-733 Jordan DC. 1984. Rhizobiaceae Conn 1938. In: KRIEG, N.R., HOLT, J.G (Eds.), Bergey’s Manual of Systematic Bacteriolgy. Williams and Wilkins, Baltimore London: pp. 235-244
  7. Matos I, Schröder EC. 1989. Strain selection for pigeon pea Rhizobium under greenhouse conditions. Plant and Soil., 116: 19-22
  8. Öğütcü H, Algur ÖF, Elkoca E, Kantar F. 2008. The Determination of Symbiotic Effectiveness of Rhizobium Strains Isolated from Wild Chickpeas Collected from High Altitudes in Erzurum. Turk. J. Agric. For., 32: 241-248
  9. Öğütçü H, Adıgüzel A, Güllüce M, Karadayı M, Şahin F. 2009. Molecular Characterization of Rhizobium Strains İsolated from Wild Chickpeas Collected from High Altitudes in Erzurum-Turkey. Romanian Biotechnological Letters., 14: 4294-4300
  10. Ögütcü H, Kasımoğlu C, Elkoca, E, 2010. Influence of Rhizobium Strains Isolated from Wild Chickpeas on the Growth and Symbiotic Performance of Chickpea (Cicer arietinum L.) under Sa