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Journals / Türk Tarım - Gıda Bilim ve Teknoloji dergisi / 2017 / Cilt: 5 - Sayı: 9

Detection of Tospoviruses in Ornamental Plants in Hobby Gardens and Landscaping Areas in Adalar District of Istanbul Province

İstanbul İli Adalar İlçesi’nde Hobi Bahçeleri ve Peyzaj Alanlarında Yetiştirilen Süs Bitkilerinde Tospovirüslerin Saptanması

Fatma ŞAFAK
MUHARREM ARAP KAMBEROĞLU
Pages
1119–1124
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Therefore, the presence of IYSV infection in Adalar was also confirmed molecularly

📌 3 Key Takeaways

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Objective & Hypothesis:This study was conducted in order to detect Tomato spotted wilt virus (TSWV), Irish yellow spot virus (IYSV) and Impatients necrotic spot virus (INSV) in ornamental plants growing in hobby gardens and landscaping areas in Adalar district (Büyükada, Heybeliada, Kınalıada and Burgazada) of Istanbul province between 2015 and 2016.
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Key Findings & Data:All of the collected samples (n=150) were firstly tested by Double Antibody Sandwich (DAS) ELISA and none of the samples were found to be infected with TSWV and INSV.
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Conclusion & Impact:Therefore, the presence of IYSV infection in Adalar was also confirmed molecularly
🔬Design:Survey & Empirical Study
📍Scope:2015 Verileri
⚙️Variables:Domain Parameters

Abstract

This study was conducted in order to detect Tomato spotted wilt virus (TSWV), Irish yellow spot virus (IYSV) and Impatients necrotic spot virus (INSV) in ornamental plants growing in hobby gardens and landscaping areas in Adalar district (Büyükada, Heybeliada, Kınalıada and Burgazada) of Istanbul province between 2015 and 2016. During the surveys carried out in that district, the samples were collected from both simptomatologically suspicious ornamental plants and the plants which did not show any symptoms. All of the collected samples (n=150) were firstly tested by Double Antibody Sandwich (DAS) ELISA and none of the samples were found to be infected with TSWV and INSV. The samples detected to be positive with IYSV by ELISA tests were then used in RT-PCR studies. At the RT-PCR using the IYSV-465c; IYSV-239f primer pair, a band with a size of 240 bp was observed for Pittosporum tobira and Hydrangea macrophylla. Therefore, the presence of IYSV infection in Adalar was also confirmed molecularly

Özet

Bu çalışma, İstanbul ili Adalar İlçesinde (Büyükada, Heybeliada, Kınalıada, Burgazada) hobi bahçeleri ve peyzaj alanlarında yetiştirilen süs bitkilerinde Tomato spotted wilt virus (TSWV), Iris yellow spot virus (IYSV) ve Impatients necrotic spot virus (INSV) varlığının saptanması amacıyla, 2015 ile 2016 yılları arasında yürütülmüştür. Bölgede yapılan survey çalışmalarında simptomolojik olarak şüpheli süs bitkileri ile simptom göstermeyen süs bitkilerinden doku örnekleri alınmıştır. Toplanan 150 adet süs bitkisi örneği öncelikle Double Antibody Sandwich (DAS) ELISA yöntemi ile testlenmiş ve örneklerin hiçbirinde TSWV ve INSV infeksiyonu saptanmamıştır. ELISA testlerinde IYSV ile bulaşık olduğu saptanan örnekler RT-PCR çalışmalarında kullanılmıştır. IYSV465c; IYSV-239f primer çifti ile yapılan RT-PCR çalışmalarında Pittosporum tobira ve Hydrangea macrophylla için 240 bp büyüklüğe sahip band gözlenmiştir. Böylece, IYSV'nin Adalar ilçesinde varlığı moleküler olarak da ortaya konulmuştur.

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Cite This Article

ŞAFAK, F., KAMBEROĞLU, M. A. (2017). Detection of Tospoviruses in Ornamental Plants in Hobby Gardens and Landscaping Areas in Adalar District of Istanbul Province. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, Cilt: 5 - Sayı: 9, 1119–1124. https://ajindex.com/articles/331549

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