Journals / Türk Tarım ve Doğa Bilimleri Dergisi / 2018 / Cilt: 5 - Sayı: 4

Investigation of Bioactive Chemicals of Passion Flower (Passiflora incarnata L.)

Çarkıfelek Çiçeğinin (Passiflora incarnata L.) Biyoaktif Kimyasallarının İncelenmesi

Pages
471–476
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to the results obtained, it has been scientifically determined that the plant can be used in the pharmaceutical industry.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, a research was conducted to determine the protease enzyme activity and the volatile organic compounds of passion flower (P. incarnata L.) to determine the usability of the plant in the pharmaceutical sector.
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Key Findings & Data:In order to determine the activity of the protease enzyme in passion flower, measurements were taken according to the method of casein digestion in the presence of 1% casein.
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Conclusion & Impact:According to the results obtained, it has been scientifically determined that the plant can be used in the pharmaceutical industry.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Çarkıfelek çiçeği · Passiflora incarnata L. · proteaz enzimi.

Abstract

In this study, a research was conducted to determine the protease enzyme activity and the volatile organic compounds of passion flower (P. incarnata L.) to determine the usability of the plant in the pharmaceutical sector. In order to determine the activity of the protease enzyme in passion flower, measurements were taken according to the method of casein digestion in the presence of 1% casein. In the light of the absorbance values, it is determined that the plant has proteolytic activity. In addition, volatile organic compounds of the plant were analyzed by headspace GC/MSD. 5 g fresh plant was taken and put into 20 ml headspace vial. The plant was extracted and analyzed with 6890 GC-5975C MSD using the HP-5 MS UI column. According to the results obtained, it has been scientifically determined that the plant can be used in the pharmaceutical industry.

Özet

Bu çalışmada, çarkıfelek çiçeğinin (P. incarnata L.) proteaz enzim aktivitesi ile uçucu organic bileşenleri belirlenerek bitkinin ilaç sektöründe kullanılabilirliğinin belirlenmesine yönelik bir araştırma yapılmıştır. Çarkıfelek çiçeğinde bulunan proteaz enziminin aktivitesini tayin etmek için %1’lik kazein varlığında kazeinin sindirimi metoduna göre ölçüm alınmıştır. Okunan absorbans değerleri ışığında bitkide proteolitik aktivite olduğu saptanmıştır. Ayrıca bitkinin uçucu organik bileşikleri headspace GC/MSD cihazı ile analiz edilmiştir. 5 g taze bitki alınarak 20 ml headspace vialine konulmuştur. Bitki ekstraksiyon ve HP-5 MS UI kolonu kullanılarak 6890 GC-5975C MSD ile analiz edilmiştir. Elde edilen sonuçlara göre bitkinin ilaç sanayinde kullanılabilir olduğu bilimsel olarak belirlenmiştir.

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Cite This Article

DÜLGER, D., ERGÜN, F., DEMİR, N. (2018). Investigation of Bioactive Chemicals of Passion Flower (Passiflora incarnata L.). Türk Tarım ve Doğa Bilimleri Dergisi, Cilt: 5 - Sayı: 4, 471–476. https://ajindex.com/articles/330481

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