Journals / Türk Tarım ve Doğa Bilimleri Dergisi / 2021 / Cilt: 8 - Sayı: 3

Effect of Co-Inoculation of Multi-Traits Bacteria Based Bio-Formulations on the Growth, Yield and Enzyme Activities of Tea

Çoklu Özellikli Bakteri Esaslı Biyo-Formüllerin Çayın Gelişim, Verim ve Enzim Aktiviteleri Üzerine Etkisi

Pages
594–604
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Bio-fertilizers formulations stimulated overall plant growth, including leaf area, green leaf yield, chlorophyll content and enzyme activities in tea leaf.

📌 3 Key Takeaways

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Objective & Hypothesis:The aims of the present study were to investigate the effectiveness of mineral fertilizer (NPK), one commercial and three N2-fixing, P-solubilizing and/or ACC deaminase-containing bacteria based bio-fertilizers in triple strains combinations (BF9: Bacillus megaterium 47/9 + Paenibacillus macquariensis RC696 + Pseudomonas fluorescens 9/7; BF10: Bacillus megaterium RC665 + Paenibacillus macquariensis RC382 + Pseudomonas fluorescens 9/7; BF11: Bacillus simplex RC64 + Pseudomonas putida 3/10 + Burkholderia pyrrocinia RC134) were evaluated for their growth and enzyme activities of tea under acidic soil conditions, in three years.
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Key Findings & Data:Bio-fertilizers formulations stimulated overall plant growth, including leaf area, green leaf yield, chlorophyll content and enzyme activities in tea leaf.
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Conclusion & Impact:This study shows that these indigenous beneficial rhizobacteria isolates have the potential to be used as microbial inoculation or bio fertilizer to stimulate tea crop growth and are promising for sustainable tea cultivation.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aims of the present study were to investigate the effectiveness of mineral fertilizer (NPK), one commercial and three N2-fixing, P-solubilizing and/or ACC deaminase-containing bacteria based bio-fertilizers in triple strains combinations (BF9: Bacillus megaterium 47/9 + Paenibacillus macquariensis RC696 + Pseudomonas fluorescens 9/7; BF10: Bacillus megaterium RC665 + Paenibacillus macquariensis RC382 + Pseudomonas fluorescens 9/7; BF11: Bacillus simplex RC64 + Pseudomonas putida 3/10 + Burkholderia pyrrocinia RC134) were evaluated for their growth and enzyme activities of tea under acidic soil conditions, in three years. The experiment was conducted in a completely randomized design with six treatments and four replicates. Bio-fertilizers formulations stimulated overall plant growth, including leaf area, green leaf yield, chlorophyll content and enzyme activities in tea leaf. In addition, inoculation with bacterial formulation, activities of the different enzymes like glutathione reductase, glutathione S-transferase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, polyphenol oxidase, peroxidase, urease, 5- dehydroshikimate reductase, and alcohol dehydrogenases also changed. The selected effective acid-tolerant multi-traits bacteria based bio-formulations could play an important role in understanding the plant tolerance and adaptation to stress, processing technology, and may contribute to the concept of the quality of tea products. This study shows that these indigenous beneficial rhizobacteria isolates have the potential to be used as microbial inoculation or bio fertilizer to stimulate tea crop growth and are promising for sustainable tea cultivation.

Özet

Bu araştırma mineral gübre, ticari mikrobiyal gübre ve azot fikseri, fosfat çözücü ve ACC deaminaze aktivitesine sahip bakteri esaslı üçlü kombinasyonlar halinde uygulanan üç farklı mikrobiyal gübre formülasyonunun (BF9: Bacillus megaterium 47/9 + Paenibacillus macquariensis RC696 + Pseudomonas fluorescens 9/7; BF10: Bacillus megaterium RC665 + Paenibacillus macquariensis RC382 + Pseudomonas fluorescens 9/7; BF11: Bacillus simplex RC64 + Pseudomonas putida 3/10 + Burkholderia pyrrocinia RC134) asidik tarla koşullarında üç yıllık sürede çay gelişim ve enzim aktiviteleri üzerine etkisinin belirlenmesi amacıyla yürütülmüştür. Deneme tesadüf bloklarında altı uygulama ve dört tekerrürlü (her bir tekerrürde beş çay öbeği) olarak kurulmuştur. Uygulanan bakteri formülasyonları yaprak alanı, yeşil yaprak verimi, klorofil içeriği ve bitki gelişimini teşvik etmiştir. Ayrıca, bakteri formülasyonu aşılamaları yaprakta; glutatyon redüktaz (GR), glutatyon S-transferaz (GST), glukoz 6-fosfat dehidrogenaz (G6PD), 6-fosfoglukonat dehidrogenaz (6PGD), polifenol oksidaz (PPO), peroksidaz (POD), 5-dehidroksişikimat redüktaz (DHSK) ve alkol dehidrogenaz (ADH), enzim aktivitelerini değiştirebilmiştir. Seçilen etkin, aside toleranslı ve çoklu özelliklere sahip bakteri esaslı biyoformülasyonlar, strese karşı bitki toleransı ve adaptasyonunu artırabilir, çay işleme teknolojisinde önemli bir rol oynayabilir ve çay ürünlerinin kalite konseptine katkıda bulunabilir. Bu çalışma, bu yerli faydalı rizobakteri izolatlarının, çay mahsulünün büyümesini teşvik etmek için mikrobiyal aşılama veya biyogübre olarak kullanılma potansiyeline sahip olduğunu ve sürdürülebilir çay yetiştiriciliği için umut verici olduğunu göstermektedir.

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Cite This Article

ÇAKMAKÇI, R., AKÇURA, S., ERAT, M. (2021). Effect of Co-Inoculation of Multi-Traits Bacteria Based Bio-Formulations on the Growth, Yield and Enzyme Activities of Tea. Türk Tarım ve Doğa Bilimleri Dergisi, Cilt: 8 - Sayı: 3, 594–604. https://ajindex.com/articles/330395

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