AI Summary & Key Takeaways
AI InsightG. 1.075,46 C, 5 min). 3) 80%> parthenogenetic eggs were obtained when the eggs were kept at 30 °C for 4 hours following keeping at 51 for 5 days before the hot water treatment. 4) The rate of apperance of parthenogenetic eggs varied widely according to the lines and it was determined that rate was higher in hybrits than the pure lines.
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Abstract
This research was conducted to determine the parthenogenetic development of silkworm unfertilized eggs (ovarian eggs). Unfertilized eggs obtained from female moths belong to the M, N pure, and Mx N, S x Shunrei hybrid lines were used. Unfertilized eggs were induced by the hot water treatment (46 C. 18 minjand three different methods tested in this research. The results were summarized as follows; 1) Rate of partheno¬genetic eggs were 81%. when the eggs were kept at 25 X". for 12 hours before the treatment and 20 °C for 3 days after the treatment. 2) 70% parthenogenetic eggs were obtained when the hot water treated eggs kept at 20 <€ for 4 days following HCI treatment (S. G. 1.075,46 C, 5 min). 3) 80%> parthenogenetic eggs were obtained when the eggs were kept at 30 °C for 4 hours following keeping at 51 for 5 days before the hot water treatment. 4) The rate of apperance of parthenogenetic eggs varied widely according to the lines and it was determined that rate was higher in hybrits than the pure lines.
Özet
Bu çalışma ipekböceğinde döllenmemiş (ovaryum özellikli) yumur¬talarda partenogenetik gelişmenin belirlenmesi amacıyla yürütülmüştür. Çalışmada M, N saf ve M x N, S x Shunrei hibrit hatlarından elde edilen döllenmemiş yumurtalar kullanılmıştır. Bu yumurtalar sıcak su mua-melesiyle(46 C, 18 dak.) uyarılmış olup araştırmada üç farklı yöntem denenmiştir. Elde edilen sonuçlar aşağıda özetlenmiştir. 1) Döllenmemiş yumurtalar muameleden önce 12 saat süre ile 25 °C sıcaklıkta ve mua¬meleden sonra 3 gün 20°C de tutuldukları zaman % 81 oranında partenogenetik yumurta ortaya çıkmıştır. 2) 46 C lik sıcak su ile muamele edilen yumurtalar 20 °C de 4 gün tutulduktan sonra HCI (1.075 özgül ağırlık, 46 C. 5 dak.) ile muamele edildikleri zaman % 72 oranında partenogenetik yumurta elde edilmiştir. 3) Yumurtalar 5XJ de 5 gün tutulduktan sonra 30° C 4 saat bekletilmişler ve sıcak suyla muamele edildiklerinde % 80 oranında partenogenetik yumurta gelişimi sağlan¬mıştır. 4) Yöntemlerde elde edilen partenogenetik yumurta yüzdesi hatlara göre farklılık göstermiş ve bu oranın hibhtlerde daha yüksek olduğu saptanmıştır.