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Journals / Ulusal Travma ve Acil Cerrahi Dergisi / 2008 / Cilt: 14 - Sayı: 2

Kritik kemik hasarlarında trombositce zengin plazma/kemik iliği kaynaklı stromal hücrelerin kompozitle enjekte edilerek tamir edilmesi: tavşanlarda deneysel çalışma

Repair of critical bone defects with injectable platelet rich plasma/bone marrow-derived stromal cells composite: experimental study in rabbits

Xiaobing CHENG
Delin LEI
Tianqiu MAO
Shuyong YANG
Fulin CHEN
Wei WU
Pages
87–95
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

RESULTS The results showed that repair of bone defect was the least in PRP alone group, and significant new bone formation could be observed in BMSCs/PRP group and particulate cancellous bone group, radiopacity area in BMSCs/PRP group attained 76.5%, which was in the same range of that in autogenous particulate cancellous bone group (82.4% in radiopacity area), compressive strength of engineered bone in BMSCs/PRP group attained 71% of that in autogenous particulate cancellous bone group (p<0.05).

📌 3 Key Takeaways

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Objective & Hypothesis:Platelet-rich plasma (PRP) has been applied to promote bone healing and developed as a novel material for bone regeneration.
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Key Findings & Data:This study aimed to investigate the feasibility of PRP carrier to deliver bone marrow derived stromal cells (BMSCs) and regenerate bone tissues to reconstruct critical bone defects in rabbits.
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Conclusion & Impact:RESULTS The results showed that repair of bone defect was the least in PRP alone group, and significant new bone formation could be observed in BMSCs/PRP group and particulate cancellous bone group, radiopacity area in BMSCs/PRP group attained 76.5%, which was in the same range of that in autogenous particulate cancellous bone group (82.4% in radiopacity area), compressive strength of engineered bone in BMSCs/PRP group attained 71% of that in autogenous particulate cancellous bone group (p<0.05).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Kafatası kırıkları · Yönlendirilmiş doku yenilenmesi · Hastalık modelleri

Abstract

Platelet-rich plasma (PRP) has been applied to promote bone healing and developed as a novel material for bone regeneration. This study aimed to investigate the feasibility of PRP carrier to deliver bone marrow derived stromal cells (BMSCs) and regenerate bone tissues to reconstruct critical bone defects in rabbits. METHODS Critical sized defect were made on eighteen rabbits' crania and treated by different composites: BMSCs/PRP (n=6); Autogenous particulate cancellous bone group (n=6) and PRP alone group (n=6). The defects were evaluated by gross observation, radiographic examination, histological examination, and mechanical examination at 12 weeks postoperatively. RESULTS The results showed that repair of bone defect was the least in PRP alone group, and significant new bone formation could be observed in BMSCs/PRP group and particulate cancellous bone group, radiopacity area in BMSCs/PRP group attained 76.5%, which was in the same range of that in autogenous particulate cancellous bone group (82.4% in radiopacity area), compressive strength of engineered bone in BMSCs/PRP group attained 71% of that in autogenous particulate cancellous bone group (p<0.05). CONCLUSION These data implicated that BMSCs delivered from PRP gel can repair bony defect in immunocompetent animals, and the tissue engineered bone in BMSCs/PRP group is comparable to autogenous particulate cancellous bone group for the repair of critical-sized bone defect.

Özet

Trombositce zengin plazma (TZP), kemik iyileşmesine katkıda bulunmak üzere uygulanmış ve kemik yenilenmesine yönelik yeni bir materyal olarak geliştirilmiştir. Bu çalışmada tavşanlarda, kemik iliği kaynaklı stromal hücreler (KİKSH) vermek ve kritik kemik iliği hasarlarını düzeltmek için kemik dokularını yenilemek üzere TZP taşıyıcılığının fizibilitesinin incelenmesi amaçlandı. GEREÇ VE YÖNTEM On sekiz tavşamn kafatasına ciddi ölçüde hasar verildi ve bunlar değişik kompozitlerle tedavi edildi: KİKSH/TZP (n=6); otojen partiküler kansellöz kemik grubu (n=6) ve tek başına TZP grubu (n=6). Hasarlar, ameliyattan sonra 12. haftada gros gözlem, radyografik, histolojik ve mekanik incelemelerle değerlendirildi. BULGULAR Kemik hasarı tamirinin tek başına TZP grubunda en az düzeyde olduğunu, KİKSH/TZP grubu ile partiküler kansellöz kemik grubunda anlamlı yeni kemik oluşumu gözlenebileceğini, radyoopasite alanının KİKSH/TZP grubunda otojen partiküler kansellöz kemik grubundakinin (radyoopasite alanında %82.4) %76.5'i oranında kazanıldığını, planlanarak düzenlenmiş kemiğin kompresif gücünün KİKSH/TZP grubunda otojen partiküler kansellöz kemik grubundakinin %71 'i oranında kazanıldığını gösterdi (p<0.05). SONUÇ TZP jelden salıverilen KİKSH'lerin, bağışıklık sistemi normal olan hayvanlarda kemik hasarını tamir edebileceğini ve KİKSH/TZP grubundaki planlanarak düzenlenmiş kemiğin kritik ölçüdeki hasarının tamirinde otojen partiküler kansellöz kemik grubundaki ile karşılaştırılabilir olduğunu göstermiştir.

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Cite This Article

CHENG, X., LEI, D., MAO, T., YANG, S., CHEN, F., WU, W. (2008). Kritik kemik hasarlarında trombositce zengin plazma/kemik iliği kaynaklı stromal hücrelerin kompozitle enjekte edilerek tamir edilmesi: tavşanlarda deneysel çalışma. Ulusal Travma ve Acil Cerrahi Dergisi, Cilt: 14 - Sayı: 2, 87–95. https://ajindex.com/articles/324781

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