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Journals / Ulusal Travma ve Acil Cerrahi Dergisi / 2011 / Cilt: 17 - Sayı: 1

Sıçanlarda oluşturulan deneysel abdominal kompartman sendromunda glutaminin oksidatif hasar üzerindeki etkileri

The effect of glutamine on oxidative damage in an experimental abdominal compartment syndrome model in rats

Deniz Necdet TİHAN
Yeşim ERBİL
Rıdvan SEVEN
Selda ARKAYA
Ümit TÜRKOĞLU
Gülçin HEPGÜL
İsmail BOROCO
Pages
1–8
DOI
—
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CONCLUSION Glutamine appears to have protective effects against reperfusion-induced oxidative damage via its anti-inflammatory and antioxidant effect.

📌 3 Key Takeaways

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Objective & Hypothesis:BACKGROUND The aim was to investigate whether or not glutamine, an antioxidant effective amino acid, improves the reperfusion-induced oxidative injury of abdominal hypertension.
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Key Findings & Data:Group 1: Abdominal compartment syndrome alone: With the rats under anesthesia, intraabdominal pressure was obtained.
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Conclusion & Impact:CONCLUSION Glutamine appears to have protective effects against reperfusion-induced oxidative damage via its anti-inflammatory and antioxidant effect.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

BACKGROUND The aim was to investigate whether or not glutamine, an antioxidant effective amino acid, improves the reperfusion-induced oxidative injury of abdominal hypertension. METHODS Wistar Albino rats were used. Group 1: Abdominal compartment syndrome alone: With the rats under anesthesia, intraabdominal pressure was obtained. Three days later, the rats were sacrificed, and intestine, lung and liver samples were removed for determination of tissue malondialdehyde (MDA) and glutathione (GSH) levels as oxidative injury parameters and of myeloperoxidase (MPO) activity as an inflammatory parameter. Trunk blood was analyzed for the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. Group 2: Abdominal compartment syndrome and glutamine: intragastric glutamine was given for seven days before and three days following establishment of the abdominal compartment syndrome model. The same examination procedure was then performed. Group 3: Glutamine administration alone. Group 4: Control group. RESULTS Intraabdominal pressure significantly increased the intestine, lung and liver MDA levels and MPO activities in comparison to the control group. Glutamine was associated with decreased MDA levels and MPO activities and increased GSH levels. CONCLUSION Glutamine appears to have protective effects against reperfusion-induced oxidative damage via its anti-inflammatory and antioxidant effect.

Özet

AMAÇ Bu çalışmada, antioksidan özelliği bilinen glutaminin kullanımının iskemreperfüzyon sonrası oluşabilecek oksidasyon hasarı üzerindeki etkilerini araştırmayı amaçladık. GEREÇ VE YÖNTEM Çalışma grubu Wistar Albino sıçanlarla oluşturuldu. Grup 1, abdominal kompartman grubuydu. Anestezi altındaki sıçanların karnına basınç uygulandı. Gaz boşaltıldıktan 3 gün sonra sıçanlar sakrifiye edildi, karaciğer, bağırsak ve akciğer doku parçalarında oksidatif hasar parametresi olarak malonildialdehit (MDA) ve glutatyon (GSH) seviyeleri ile enflamatuvar parametre olarak miyeloperoksidaz (MPO) aktivitesi ölçüldü. Alınan kan örneklerinde serum alanin-aminoasit transferaz (ALT) ve aspartat-aminoasit transferaz (AST) seviyeleri ölçüldü. Grup 2, abdominal kompartman ve glutamin uygulanan gruptu. Sıçanlara 1 hafta boyunca yüksek doz glutamin mide gavajı yolu ile verildi. Gavaj işlemi sonrası ilk gruptaki batın içi basınç uygulaması yapıldı, 3 gün daha glutamin verilen sıçanlar sakrifiye edildi, Grup 1?deki işlemlerin aynısı yapıldı. Grup 3 glutamin grubuydu. Grup 4 sham kontrol grubuydu. BULGULAR Abdominal kompartman yaratılan sıçanlarda MDA düzeyi ve MPO aktivitesi kontrol grubuna göre anlamlı oranda artmıştı. Glutamin ile MDA düzeyi ve MPO aktivitesi azaldı, GSH seviyesi arttı. SONUÇ Oksidatif hasar parametrelerine bakıldığında, glutaminin, reperfüzyon hasarını azaltıcı etkisinin antienflamatuvar ve antioksidan etkilerine bağlı olduğu düşünülmüştür.

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Cite This Article

TİHAN, D. N., ERBİL, Y., SEVEN, R., ARKAYA, S., TÜRKOĞLU, Ü., HEPGÜL, G., BOROCO, İ. (2011). Sıçanlarda oluşturulan deneysel abdominal kompartman sendromunda glutaminin oksidatif hasar üzerindeki etkileri. Ulusal Travma ve Acil Cerrahi Dergisi, Cilt: 17 - Sayı: 1, 1–8. https://ajindex.com/articles/324202

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References (17)

  1. Cullen DJ, Coyle JP, Teplick R, Long MC. Cardiovascular, pulmonary, and renal effects of massively increased intraabdominal pressure in critically ill patients. Crit Care Med 1989;17:118-21
  2. Chen RJ, Fang JF, Chen MF. Intra-abdominal pressure moni- toring as a guideline in the nonoperative management of blunt hepatic trauma. J Trauma 2001;51:44-50
  3. Asensio JA, Ceballos J, Forno W. Intra-abdominal pressure monitoring. In: WC Shoemaker, GC Velhamos, D Demetriades, editors. Procedures and monitoring for the critically ill. Philadelphia: W.B. Saunders; 2002. p. 99-103
  4. Ivatury RR, Diebel L, Porter JM, Simon RJ. Intra-abdominal hypertension and the abdominal compartment syndrome. Surg Clin North Am 1997;77:783-800
  5. Kumar V, Cotran RS, Robbins SL. Basic pathology. Philadelphia: W.B. Saunders; 1992. p. 4-11
  6. Clarkson AN, Sutherland BA, Appleton I. The biology and pathology of hypoxia-ischemia: an update. Arch Immunol Ther Exp (Warsz) 2005;53:213-25. 7. de Groot H, Rauen U. Ischemia-reperfusion injury: processes in pathogenetic networks: a review. Transplant Proc 2007;39:481-4
  7. Ley K, Reutershan J. Leucocyte-endothelial interactions in health and disease. Handb Exp Pharmacol 2006:97-133
  8. Gutteridge JM, Halliwell B. Free radicals and antioxidants in the year 2000. A historical look to the future. Ann N Y Acad Sci 2000;899:136-47
  9. Rodwell VW. Metabolism of proteins and aminoacids. In: Murray RK, Granner DK, Mayes PA, Rodwell VW, editors. Harper’s biochemistry. Appleton&Lange; 1990. p. 333-412
  10. Tapiero H, Mathé G, Couvreur P, Tew KD. II. Glutamine and glutamate. Biomed Pharmacother. 2002;56:446-57
  11. Young VR, Ajami AM. Glutamine: the emperor or his clothes? J Nutr 2001;131:2449S-87S
  12. Guloglu R, Berber E, Taviloglu K, Gunay K, Ertekin C. Clinical importance of intraabdomainal pressure in the sugical intensive care. Eur J Emerg Surg Int Care 1997;20:191-4
  13. Saggi BH, Sugerman HJ, Ivatury RR, Bloomfeld GL. Abdominal compartment syndrome. J Trauma 1998;45:597-609
  14. Van Hee R. An abdominal challenge: the compartment syndrome. G Chir 2007;28:413-8
  15. Eddy V, Nunn C, Morris JA Jr. Abdominal compartment syndrome. The Nashville experience. Surg Clin North Am 1997;77:801-12
  16. Schein M, Wittmann DH, Aprahamian CC, Condon RE. The abdominal compartment syndrome: the physiological and clinical consequences of elevated intra-abdominal pressure. J Am Coll Surg 1995;180:745-53
  17. Mendoza-Sagaon M, Hanly EJ, Talamini MA, Kutka MF, Gi