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Journals / Ulusal Travma ve Acil Cerrahi Dergisi / 2012 / Cilt: 18 - Sayı: 6

Sıçan omurilik yaralanma modelinde dinlenmiş sarımsak özütünün etkileri

Effects of the aged garlic extract on spinal cord injury model in rat

Berker CEMİL
Emre Cemal GÖKÇE
Hüsamettin ERDAMAR
Ayça KARABÖRK
Özlem ONUR
Aylin HEPER OKÇU
Ramazan YİĞİTOĞLU
Bülent ERDOĞAN
Pages
463–468
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Further studies with different experimental settings are required to achieve conclusive results.

📌 3 Key Takeaways

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Objective & Hypothesis:BACKGROUND Aged garlic extract (AGE) is a potent antioxidant agent with an established neuroprotective effect in cerebral isch- emia.
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Key Findings & Data:METHODS Spinal cord trauma was applied to 19 adult male Wistar rats using the clip compression method.
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Conclusion & Impact:Further studies with different experimental settings are required to achieve conclusive results.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

BACKGROUND Aged garlic extract (AGE) is a potent antioxidant agent with an established neuroprotective effect in cerebral isch- emia. However, the potential protective effect of AGE in spinal cord injury (SCI) is still unknown. METHODS Spinal cord trauma was applied to 19 adult male Wistar rats using the clip compression method. Animals were divided into three groups. Animals in the AGE group were administered 250 mg/kg per day of AGE diluted in tap water orally by gavage for 15 days prior to trauma. After spi- nal cord trauma, malondialdehyde (MDA) and superoxide dismutase (SOD) levels of the AGE group were compared with the animals in the control and SCI groups. The animals were examined by inclined plane 24 hours (h) after the trauma. At the end of the experiment, spinal cord tissue samples were harvested for pathological evaluation. RESULTS Regarding tissue MDA and SOD levels after trauma, animals in the AGE group demonstrated decreased MDA levels and increased SOD levels when compared with the SCI group. However, these results were no better than in the control group. The AGE group demonstrated better pathological findings than the SCI group. The result regarding the functional finding was similar. CONCLUSION AGE demonstrated neuroprotective effects in SCI. Further studies with different experimental settings are required to achieve conclusive results.

Özet

AMAÇ Dinlenmiş sarımsak özütü (aged garlic extract - AGE) serebral iskemide nöroprotektif etkileri gösterilmiş güçlü bir antioksidan ajandır. Ancak AGE’nin omurilik yaralanmasında potansiyel koruyucu etkileri halen bilinmemektedir. GEREÇ VE YÖNTEM Wistar cinsi 19 yetişkin erkek sıçana klip kompresyon metodu ile omurilik travması uygulandı. Hayvanlar üç gruba ayrıldı. Travmadan 15 gün once sıçanlara gavajla oral yolla 250 mg/kg’a AGE suda çözülerek verildi. Omurilik yaralanması sonrası AGE verilen gruptaki sıçanların malondi- aldehit (MDA) ve süperoksitit dismutaz (SOD) seviyeleri, kontrol grubundaki ve sadece omurilik travması uygulanan gruptaki sıçanlarla karşılaştırıldı. Hayvanlar travma sonrası 24. saatte eğik düzlemde değerlendirildi. Deney sonrası omurilik doku örnekleri patolojik incelemeye gönderildi. BULGULAR Dokulardaki MDA ve SOD seviyeleri incelendiğinde, AGE verilmiş ve omurilik travması uygulanmış gruptaki sıçanlarda, sadece omurilik travması uygulanmış sıçanlara göre azalmış MDA ve artmış SOD seviyeleri saptandı. Bununla birlikte sonuçlar kontrol grubundakilere göre daha iyi değildi. AGE grubun patolojik bulguları omurilik yaralanması grubuna göre daha iyiydi. Aynı zamanda fonksiyonel değerlendirme bulguları benzer sonuçlar gösteriyordu. SONUÇ AGE, omurilik yaralanmasında koruyucu etkiler göstermiştir. AGE’nin etkinliği ile ilgili daha ileri ve farklı deneysel çalışmalara ihtiyaç duyulmaktadır.

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Cite This Article

CEMİL, B., GÖKÇE, E. C., ERDAMAR, H., KARABÖRK, A., ONUR, Ö., OKÇU, A. H., YİĞİTOĞLU, R., ERDOĞAN, B. (2012). Sıçan omurilik yaralanma modelinde dinlenmiş sarımsak özütünün etkileri. Ulusal Travma ve Acil Cerrahi Dergisi, Cilt: 18 - Sayı: 6, 463–468. https://ajindex.com/articles/323910

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