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Journals / Uluslararası Hematoloji-Onkoloji Dergisi / 2009 / Cilt: 19 - Sayı: 1

Kolorektal kanserli bir grup Türk hastasında VDR geni ApaI ve TaqI polimorfizmlerinin belirlenmesi

Determination of ApaI and TaqI polymorphisms of VDR Gene in a group of Turkish patients with colorectal cancer

FUAT DİLMEÇ
Abdullah ÖZGÖNÜL
FERİDUN AKKAFA
ALİ UZUNKÖY
André B.P. Van KUILENBURG
Pages
18–22
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The investigated polymorphisms in the VDR gene do not represent a significant risk factor for CRC in our population.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Colorectal cancer (CRC) is the leading cause of cancer death among human around the world.
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Key Findings & Data:The vitamin D receptor gene (VDR) is a member of the nuclear receptor super family, which is expressed in the tissue of gastrointestinal tract, known to modulate the rate of cell proliferation.
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Conclusion & Impact:The investigated polymorphisms in the VDR gene do not represent a significant risk factor for CRC in our population.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Kolorektal neoplazmlar · Taq polimeraz · Polimorfizm

Abstract

Colorectal cancer (CRC) is the leading cause of cancer death among human around the world. The vitamin D receptor gene (VDR) is a member of the nuclear receptor super family, which is expressed in the tissue of gastrointestinal tract, known to modulate the rate of cell proliferation. We aimed to investigate the genotype and allele frequencies and association of the VDR gene: c.1025-49G>T (ApaIG>T) and c.1056T>C (TaqIT>C) polymorphisms with CRC in Turkish patients. Fifty-six patients with CRC and 169 healthy individuals were enrolled to study, and their DNA was isolated. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to detect the frequency of c.1025-49G>T and c.1056T>C polymorphisms. The prevalence of the c.1025-49G>T and c.1056T>C alleles and the genotype frequencies in patients with CRC was similar to that in the normal population. The investigated polymorphisms in the VDR gene do not represent a significant risk factor for CRC in our population.

Özet

Kolorektal kanser (KRK), Dünyada insanlar arasında kanserden ölümün önde gelen sebebidir. Vitamin D reseptör (VDR) geni, mide bağırsak dokusunda ifadesini bulan ve hücre bölünme oranını düzenleyen çekirdeğe ait reseptör büyük ailesinin bir üyesidir. Türk hastalarında KRK ile VDR geni: c.1025-49G>T (ApaIG>T) ve c.1056T>C (TaqIT>C) polimorfizmlerinin ilişkisini, genotip ve allel oranlarını araştırmayı amaçladık. KRK’li 56 hasta ve 169 sağlıklı birey çalışmaya dahil edildi ve bunların DNA’sı izole edildi. c.1025-49G>T ve c.1056T>C polimorfizmlerinin belirlenmesinde, Polimeraz zincir reaksiyonu-restriksiyon parça uzunluk polimorfizm (PCR-RFLP) tekniği kullanıldı. KRK’li hastalarda c.1025-49G>T ve c.1056T>C allel ve genotip frekanslarının yaygınlığı, normal popülasyondaki değerlerle benzerdir. VDR geninde araştırılan polimorfizmleri, toplumumuzda KRK için anlamlı bir risk faktörü olduğunu göstermiyor.

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Cite This Article

DİLMEÇ, F., ÖZGÖNÜL, A., AKKAFA, F., UZUNKÖY, A., KUILENBURG, A. B. V. (2009). Kolorektal kanserli bir grup Türk hastasında VDR geni ApaI ve TaqI polimorfizmlerinin belirlenmesi. Uluslararası Hematoloji-Onkoloji Dergisi, Cilt: 19 - Sayı: 1, 18–22. https://ajindex.com/articles/323007

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