Journals / Selçuk Tıp Dergisi / 2007 / Cilt: 23 - Sayı: 2

Tissue element levels in rats and postmortem interval

Sıçanlarda doku element seviyeleri ve postmortem interval

Pages
57–67
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: It was concluded that, the availability of tissue element levels as a reliable method in the determination of postmortem interval needs to be revealed by experimental studies that will be done on wider series and the analyses on the autopsy material.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: The aim of this experimental study is to determine the relationship of the element levels in different tissues and the postmortem process and the reliability of this relationship in the determination of postmortem interval.
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Key Findings & Data:Material and method: For this purpose, 52 three-month-old male Sprague-Dawley rats were sacrified by cervical dislocation and four of them were seperated for dissection.
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Conclusion & Impact:Conclusion: It was concluded that, the availability of tissue element levels as a reliable method in the determination of postmortem interval needs to be revealed by experimental studies that will be done on wider series and the analyses on the autopsy material.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Erkek · Sıçanlar · Modeller

Abstract

Aim: The aim of this experimental study is to determine the relationship of the element levels in different tissues and the postmortem process and the reliability of this relationship in the determination of postmortem interval. Material and method: For this purpose, 52 three-month-old male Sprague-Dawley rats were sacrified by cervical dislocation and four of them were seperated for dissection. On the other hand, remaining 48 rats were divided into two groups for the purpose of investigating surrounding temperature the corps existed to the tissue element levels. One of the groups was kept at 4 °C and the other at 18±2 °C. Just after sacrifice, seperated four rats were dissected. Then the dissection was made at 6., 12., 24., 48., 72. and 96. hours four rats each among the groups kept in both temperatures. Brain, myocardium, liver, kidney and skeletal muscle sampleswere taken from each rat. Element levels were determined by ICP-AES in the samples which were prepared for analysing with microwave digestion method. The values acquired for Ca, Cu, Fe, K, Mg, Na, P, S and Zn were statistically evaluated by Kruskal-Wallis and Mann-Whitney tests. Results: When the findings of both temperatures were evaluated together in all tissues, it was determined that Fe, K, Na, Ca and Cu showed significant changes in more than one tissue at 4 °C and 18±2 °C. Conclusion: It was concluded that, the availability of tissue element levels as a reliable method in the determination of postmortem interval needs to be revealed by experimental studies that will be done on wider series and the analyses on the autopsy material.

Özet

Amaç: Bu deneysel çalışmanın amacı, farklı dokulardaki element seviyelerinin postmortem süreç ile ilişkisini ve postmortem interval saptanmasındaki kullanılabilirliğini araştırmaktır. Gereç ve yöntem: Bu amaçla, 52 adet üç aylık Sprague-Dawley cinsi erkek sıçana servikal dislokasyon uygulandıktan sonra 4'ü hemen diseke edildi. Diğer 48 sıçan ise, cesedin kaldığı ortam ısısının doku element seviyelerine etkisinin araştırılması amacıyla iki gruba ayrıldı. Gruplardan biri 4 °C'de, diğeri 18±2 °C'de bekletildi. Daha sonra her iki ısıda bekletilen sıçanlardan; 6., 12., 24., 48., 72. ve 96. saatlerde 4'er sıçan diseke edildi. Her sıçandan beyin, miyokard, karaciğer, böbrek ve iskelet kası örnekleri alındı. Örnekler, mikrodalga yakma yöntemiyle analize hazır hale getirilerek, ICP-AES cihazında element düzeyleri ölçüldü. Ca, Cu, Fe, K, Mg, Na, P, S ve Zn elementlerine ait değerler, istatistiksel olarak Kruskal-Wallis ve Mann-Whitney testleri ile değerlendirildi. Bulgular: Tüm dokularda, her iki ısıdaki bulgular ortak olarak değerlendirildiğinde; Fe, K, Na, Ca ve Cu'ın birden çok dokuda 4 °C ve 18±2 °Clerde anlamlı değişim gösterdiği saptandı. Sonuç: Postmortem interval saptanmasında doku element seviyelerinin güvenilir bir yöntem olarak kullanılabilirliğinin, daha geniş serilerde yapılacak deneysel çalışmalar ve otopsi materyalinde yapılacak analizler ile ortaya çıkartılması gerektiği sonucuna varıldı.

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Cite This Article

DOĞAN, K. H., GÜNAYDIN, G., DEMİRCİ, Ş. (2007). Tissue element levels in rats and postmortem interval. Selçuk Tıp Dergisi, Cilt: 23 - Sayı: 2, 57–67. https://ajindex.com/articles/319557

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