SavedAdd your journal

Journals / Selçuk Tıp Dergisi / 2018 / Cilt: 34 - Sayı: 1

Hidrojen Peroksit Uygulanan Sıçanlarda Beyin Kolesterol, A ve E Vitamini Düzeyine Geraniolün Etkisi

Effect of Geraniol on Brain Cholesterol, Vitamin A and E Levels in The Hydrogen Peroxide-Treated Rats

Zafer ŞAHİN
Ahmet ÖZKAYA
Ökkeş YILMAZ
Ahmet Orhan GÖRGÜLÜ
Pages
18–22
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Geraniolün erkek sıçanlarda $H_2O_2$’nin etkilerini azaltma potansiyeli oldugu söylenebilir

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Amaç: Bir monoterpen olan geraniol, bazı bitkilerde yer alan dogal bir antioksidandır.
📊
Key Findings & Data:Hidrojen peroksit ($H_2O_2$) ise reaktif oksijen türlerinin (ROS) bir üyesidir.
💡
Conclusion & Impact:Geraniolün erkek sıçanlarda $H_2O_2$’nin etkilerini azaltma potansiyeli oldugu söylenebilir
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Amaç: Bir monoterpen olan geraniol, bazı bitkilerde yer alan dogal bir antioksidandır. Hidrojen peroksit ($H_2O_2$) ise reaktif oksijen türlerinin (ROS) bir üyesidir. Bu çalısmada, $H_2O_2$ uygulanan sıçanlarda geraniolün beyin kolesterolü ve yagda çözünen A (retinol) ve E (alfa (α)-tokoferol ve α-tokoferol asetat) vitaminlerine etkisinin belirlenmesi amaçlanmıstır. Gereçler ve Yöntem: Çalısmada yetiskin erkek Wistar sıçanlar rastgele dört gruba ayrılarak (n=7), geraniol (50mg/kg) ve $H_2O_2$ (16mg/kg), intraperitoneal olarak, 30 gün süresince gün asırı uygulandı. Beyin numuneleri homojenize edildikten sonra kolesterol, α-tokoferol, α-tokoferol asetat ve retinol düzeyleri yag ekstraktlarından HPLC ile analiz edildi. Bulgular: Beyin kolesterol düzeyinin $H_2O_2$ grubunda kontrol grubuna kıyasla daha fazla oldugu belirlendi. $H_2O_2$ ve geraniol+$H_2O_2$ gruplarında, beyin α-tokoferol asetat düzeylerinin kontrole kıyasla daha yüksek oldugu bulundu. Beyin retinol seviyesi bakımından grupların hiçbirisinde istatistiksel olarak anlamlı bir degisiklik mevcut degildi. Sonuç: Elde ettigimiz veriler, beyinde kolesterol ve α-tokoferol asetat düzeylerinin $H_2O_2$ ile olusturulan oksidatif stresten etkilendigini göstermektedir. Geraniolün erkek sıçanlarda $H_2O_2$’nin etkilerini azaltma potansiyeli oldugu söylenebilir

Özet

Aim: Geraniol, a monoterpene, is a plant-derived natural antioxidant. Hydrogen peroxide ($H_2O_2$) is a member of reactive oxygen species (ROS). The objective of this study is to de-termine the effect of geraniol on brain cholesterol and lipophilic vitamins such as vitamin A (retinol) and vitamin E (alpha (α)- tocopherol and α-tocopherol acetate) in $H_2O_2$ -administrated male rats. Materials and Methods: Adult male Wistar rats were randomly divided into four groups (n=7). Geraniol (50 mg/kg) and $H_2O_2$ (16 mg/kg) were administered by an intraperitoneal injection for 30 days with a 1-day interval. Brain samples were homogenized and cholesterol, α-tocopherol, α-tocopherol acetate, and retinol levels analyzed from lipid extracts by HPLC. Results: Brain cholesterol level was found to be significantly higher in the $H_2O_2$ group than the control group. Brain α-tocopherol acetate levels were found to be significantly higher in the $H_2O_2$ and the geraniol+$H_2O_2$ groups than the control group. There was no difference in retinol levels between any groups. Conclusion: In conclusion, our results indicate that brain cholesterol and α-tocopherol acetate levels are affected by $H_2O_2$-induced oxidative stress. Moreover, we suggest that geraniol has the potential to reduce the effect of $H_2O_2$ in male rats.

🔖

Cite This Article

ŞAHİN, Z., ÖZKAYA, A., YILMAZ, Ö., GÖRGÜLÜ, A. O. (2018). Hidrojen Peroksit Uygulanan Sıçanlarda Beyin Kolesterol, A ve E Vitamini Düzeyine Geraniolün Etkisi. Selçuk Tıp Dergisi, Cilt: 34 - Sayı: 1, 18–22. https://ajindex.com/articles/319101

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Tiwari M, Kakkar P. Plant derived antioxidants - Geraniol and camphene pro-tect rat alveolar macrophages against t-BHP induced oxidative stress. Toxicol in vitro 2009;23(2):295-301
  2. Takechi H, Miyazawa M. Biotransformation of Geraniol by the larvae of com-mon cutworm (Spodoptera litura). J Oleo Sci 2006;55(3):143-9
  3. Chen W, Viljoen AM. Geraniol - A review of a commercially important fra-grance material. South African J Botany 2010;76(4):643-51
  4. Wiseman DA, Werner SR, Crowell PL. Cell cycle arrest by the isoprenoids perillyl alcohol, geraniol, and farnesol is mediated by p21(Cip1) and p27(Kip1) in human pancreatic adenocarcinoma cells. J Pharmacol Exp Ther 2007;320(3):1163-70
  5. Ozkaya A, Sahin Z, Gorgulu AO, et al. Geraniol attenuates hydrogen perox-ide-induced liver fatty acid alterations in male rats. J Intercult Ethnopharmacol 2016;6(1):29-35
  6. Dietschy JM, Turley SD. Thematic review series: Brain lipids. Cholesterol metabolism in the central nervous system during early development and in the mature animal. Lipid Res 2004;45:1375-97
  7. Björkhem I, Meaney S. Brain cholesterol: Long secret life behind a barrier. Arterioscler Thromb Vasc Biol 2004;24:806- 15
  8. Björkhem I, Lütjohann D, Breuer O, et al. Importance of a novel oxidative mechanism for elimination of brain cholesterol. Turnover of cholesterol and 24(S)-hydroxycholesterol in rat brain as measured with 18O2 techniques in vivo and in vitro. J Biol Chem 1997;272(48):30178-84
  9. Lund EG, Guileyardo JM, Russell DW. cDNA cloning of cholesterol 24-hydroxylase, a mediator of cholesterol homeostasis in the brain. Proc Natl Acad Sci USA 1999;96(13):7238-43
  10. Martín MG, Pfrieger F, Dotti CG. Cholesterol in brain disease: sometimes de-terminant and frequently implicated. EMBO Rep 2014;15(10):1036-52
  11. Shulman RG, Rothman DL, Behar KL, et al. Energetic basis of brain activity: implications for neuroimaging, Trends Neurosci 2004;27:489-95
  12. Juurlink BH, Paterson PG. Review of oxidative stress in brain and spinal cord injury: suggestions for pharmacological and nutritional management strate-gies. J Spinal Cord Med 1998;21:309-34
  13. Canter PH, Wider B, Ernst E. The antioxidant vitamins A, C, E and selenium in the treatment of arthritis: a systematic review of randomized clinical trials. Rheumatology (Oxford) 2007;46(8):1223-33
  14. Gamba P, Testa G, Gargiulo S, Set al. Oxidized cholesterol as the driving force behind the development of Alzheimer’s disease. Front Aging Neurosci 2015;