Journals / Selçuk Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi / 2004 / Sayı: 24

Mastitisli ineklerden izole edilen koagulaz pozitif Staphylococcus türlerinin antimikrobiyal dirençliliği ve plazmit tiplendirmesi

Antimicrobial resistance and plasmid typing of coagulase positive Staphylococcus species isolated from bovine mastitis

Pages
45–50
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study suggests that plasmid profile analysis does not appear to be a suitable method for typing S.aureus and S.intermedius.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, a number of 50 Coagulase positive Staphylococcus isolates has been isolated and identified using conventional bacteriological methods from bovine mastitis.
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Key Findings & Data:Of the total isolates 40 was identified as Staphylococcus aureus and 10 was identified as Staphylococcus intermedius.
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Conclusion & Impact:This study suggests that plasmid profile analysis does not appear to be a suitable method for typing S.aureus and S.intermedius.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Staphylococcus aureus · Staphylococcus intermedius · meme yangısı

Abstract

In this study, a number of 50 Coagulase positive Staphylococcus isolates has been isolated and identified using conventional bacteriological methods from bovine mastitis. Of the total isolates 40 was identified as Staphylococcus aureus and 10 was identified as Staphylococcus intermedius. This study indicates the increasing significance of S.intermedius as the causative agents of bovine subclinical mastitis. All the Staphylococcus strains were examined for their susceptibilities to antibiotics and plasmid profiles. Plasmid profiling demonstrated that 38 of 40 S.aureus and 9 of 10 S.intermedius isolates contained plasmid. Molecular weight of plasmids varied from 1.1 to 19 kb. The highest resistance to chematerapotics used was observed to penicillin. This study suggests that plasmid profile analysis does not appear to be a suitable method for typing S.aureus and S.intermedius.

Özet

Bu çalışmada, 50 koagulaz pozitif Staphylococcus izolatı mastitisli ineklerden konventiyonal bakteriyolojik metodlar kullanılarak izole edilmiş ve tanımlanmıştır. Toplam izolattan 40 tanesi Staphylococcus aureus olarak ve 10 tanesi ise Staphylococcus intermedius olarak tanımlanmıştır. Bu çalışma, subklinik mastitisli ineklerin etken ajanlar olarak S. intermedius’un öneminin arttışını göstermiştir. Bütün Staphylococcus suşlarının antibiyotiklere duyarlılıkları ve plazmit profilleri incelenmiştir. Plazmit profili 40 S. aureus izolatının 38’nin ve 10 S. intermedius izolatının 9’nun plazmit içerdiğini göstermiştir. Plazmitlerin moleküler ağırlıkları 1.1 den 19 kb’a kadar değişmektedir. Kullanılan kemoterapotiklere en yüksek dirençlilik penisilinde gözlenmiştir. Bu çalışma, plazmit profili analizinin S. aureus ve S. intermedius’un tiplendirilmesi için uygun bir metod olmadığını göstermektedir.

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Cite This Article

ARSLAN, E., AÇIK, L., UÇAN, U. S., ÇELEBİ, A. (2004). Mastitisli ineklerden izole edilen koagulaz pozitif Staphylococcus türlerinin antimikrobiyal dirençliliği ve plazmit tiplendirmesi. Selçuk Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi, Sayı: 24, 45–50. https://ajindex.com/articles/317592

References (1)

  1. [I] Gudding R, McDonald JS, Cheville NF (1984) Pathogenesis of Staphylococcus aureus, bacteriological, histologic, and ultrastructural pathologic findings. Am J Vet Res 45, 2525-2531. [2] Francis GP, Carrol PJ (1986) Antibiotic resistance patterns of Staphylococcus aureus strains isolated from clinical bovine mastitis. Vet. Rec 188: 361-366. [3] Linton AH (1984) Antibiotic resistance in Staphylococcus aureus and Streptococcus. Brit Med Bull 40: 7781. [4] Moellering RC (1998) Antibiotic resistance: lessons for the future. Clin Inf Dis 27, 135-140. [5] Parisi JT, Hecht DW (1980) Plasmid profiles in epidemiologic studies of infections by S. epidermidis. J Inf Dis 141, 637-643. [6] Wachsmuth I, Kiehlbauch J, Bopp C, Cameron D, Strockbine N Wells JABP (1991) The use of plasmid profiles and nucleic acid probes in epidemiologic investigations of foodborne, diarrheal diseases, Inter J Food Microbiol'12, 77-90. [7] Dorn, C, Silapanuntakul R, Angrick E, Shipman L (1992). Plasmid analysis and epidemiology of Salmonella enteritidis infection in three commercial layer flocks, Avian Dis 38, 844-851. [8] Neuwirth C, Siebor E, Lopez J, Pechinot A, Kazmierczak A (1996) Outbreak of TEM-24-producing Enterobacter aerogenes in an intensive care unit and dissemination of the extended-spectrum beta-lactamase to other members of the family Enterobacteriaceae, J Clin Microbiol 34, 76-79. [9] Shi ZH, Liu PYF, Lau YJ, Hu BS, Lin YH (1996) Comparison of polymerase chain reaction and pulsed-field gel electrophoresis for the epidemiological typing of Campylobacter jejuni, Diag Microbiol Inf Dis 26, 103-108. [10] McClure PJ (2000). Microbiological hazard identification in the meat industry. HACCP in the meat industry. (M Brown, Ed), CRC Press Woodhead Publishing Limited, Cambridge, 157-176. [11] Honkanen-Buzalski T (1990) The role of coagulase-negative Staphylococcal species in bovine mastitis. International Symposium on Bovine Mastitis, Indianapolis, USA. September 13-16, 98-102. [12] Collins CH, Carter GR, Chengappa MM (1991) Essentials of Veterinary Bacteriology and Micology. 4th Ed. Lea & Febiger, London. [13] Erganiş O, Kuyucuoglu Y, Ok U (1995) İnek ve koyun mastitislerine sebep olan koagulaz negatif ve pozitif staphylococ'\ann biyotiplendirilmesi. Veterinarium. 6, 23-27. [14] Bauer AV, Kirby WMM, Sherris JC, Turc M (1966) Antibiotic susceptibility testing by a standardized single disc method. J Clin Pathol 45, 493-494. [15] Maniatis R., Fritsh EF, Sambrook J (1982). A laboratory