SavedAdd your journal

Journals / Solunum / 2007 / Cilt: 9 - Sayı: 3

Evaluation of lipid peroxidation and antioxidant enzyme activities in the serum of adult asthma patients

Yetişkin astımlı hasta serumlarında lipid peroksidasyonu ve antioksidan enzim aktivitelerinin değerlendirilmesi

Neriman AKSU
Ferah ARMUTÇU
Levent KART
Ahmet GÜREL
Nejat DEMİRCAN
Murat ÜNALACAK
Journal
Solunum
Pages
153–157
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results suggest that oxidative and nitrosative stress result in lipid peroxidation and alterations in antioxidant enzymes in asthma patients.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In recent years, free radical damage and insufficiency of antioxidant defense system has been gradually accused of the pathogenesis of many diseases.
📊
Key Findings & Data:Seventy mild-moderate asthmatic adults and seventy healthy adults were enrolled in the study for this aim.
💡
Conclusion & Impact:These results suggest that oxidative and nitrosative stress result in lipid peroxidation and alterations in antioxidant enzymes in asthma patients.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Lipid peroksidasyonu · Ksantin oksidaz · Tiyobarbitürik asit reaktif maddeleri

Abstract

In recent years, free radical damage and insufficiency of antioxidant defense system has been gradually accused of the pathogenesis of many diseases. One of these diseases is bronchial asthma. In this study we aimed to investigate the role of oxidative damage and antioxidant system in the pathogenesis of bronchial asthma. Seventy mild-moderate asthmatic adults and seventy healthy adults were enrolled in the study for this aim. The levels of thiobarbituric acid-reactive substance (TBARS) and nitric oxide (NO) and the activities of xanthine oxidase (XO), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured in serum of patients and control subjects. The mean TBARS (p < 0.01) and NO (p < 0.05) values and XO (p < 0.01) activity were significantly higher in the serum of the patients compared to the control group. The mean values of serum SOD (p < 0.01) and GSH-Px (p < 0.05) enzymatic activities were significantly lower in the patients compared to the control group. These results suggest that oxidative and nitrosative stress result in lipid peroxidation and alterations in antioxidant enzymes in asthma patients.

Özet

Son yıllarda, pek çok hastalığın patogenezinde veya sonucunda giderek artan bir şekilde, serbest radikal hasarı ve antioksidan savunma sistemlerindeki yetersizlik suçlanmaktadır. Bu hastalıklardan biri de bronş astımıdır. Bu çalışmada oksidatif hasarın ve antioksidan sistemdeki değişimlerin astımdaki düzeylerinin incelenmesi amaçlandı. Hafif-orta şiddette astımlı 70 hasta ve kontrol grubu olarak 70 sağlıklı erişkin bu amaçla çalışmaya alındı. Hasta ve kontrol grubu serumlarında tiyobarbitürik asitle reaksiyon veren madde (TBARS) ve nitrik oksit (NO) düzeyleri ile, ksantin oksidaz (XO), süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GSH-Px) aktiviteleri ölçüldü. Hasta grubunda serum TBARS (p < 0.01) ve NO (p < 0.05) düzeyleri ile XO (p < 0.01) aktivitesi kontrol grubuna göre anlamlı düzeyde yüksek bulundu. Hasta grubu serum SOD (p < 0.01) ve GSH-Px (p < 0.05) enzim aktiviteleri kontrol grubuna göre anlamlı derecede düşük bulundu. Bu sonuçlar bronşial astımlı hastalarda oksidatif ve nitrozatif stresin lipid peroksidasyonu ve antioksidan enzimlerde değişikliklere neden olduğunu düşündürmektedir.

🔖

Cite This Article

AKSU, N., ARMUTÇU, F., KART, L., GÜREL, A., DEMİRCAN, N., ÜNALACAK, M. (2007). Evaluation of lipid peroxidation and antioxidant enzyme activities in the serum of adult asthma patients. Solunum, Cilt: 9 - Sayı: 3, 153–157. https://ajindex.com/articles/314425

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (18)

  1. Dworski R. Oxidant stress in asthma. Thorax 2000; 55: 51- 3
  2. McBride ED, Koening OJ, Luchtel LD, Williams VP, Handerson RW. Inflammatory effects of ozone in the upper airways of subjects with asthma. Am J Respir Crit Care Med 1994; 149: 1192- 7
  3. Andreadis AA, Hazen SL, Comhair SAA, Erzurum SC. Oxidative and nitrosative events in asthma. Free Radic Biol Med 2003; 35: 213 25
  4. Rahman I, Morrison D, Donaldson K, MacNee W. Systemic oxidative stres in asthma, chronic obstructive pulmonary disease, and smokers. AmJ Respir Crit Care Med 1996; 154; 1055-60
  5. Rahman I, Smith CA, Lawson MF, Harrison DJ, MacNee W. Induction of gamma glutamylcysteine synthetase by cigarette smoke is associated with AP-1 in human alveolar epitelial cells. FEBS Lett 1996; 396: 21-5
  6. Henricks PAJ, Nijkamp FP. Reactive oxygen species as mediators in asthma. Pulm Pharmacol Ther 2001; 14: 409- 21
  7. Halliwell B. Free radicals, antioxidant and human disease. Curiosity, cause or consequence ? Lancet 1994; 344: 721-4
  8. Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 1990; 186: 421 - 31
  9. Cortas NK, Wakid NW. Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. Clin Chem 1990; 36: 1440-3
  10. Prajda N, Weber G. Malignant transformation-linked imbalance: decreased XO activity in hepatomas. FEBS Lett 1 975; 59: 245- 9
  11. Sun Y, Oberley LW, Ying L A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497- 500
  12. Durak I, Yurtaslani Z, Canbolat O, Akyol O. A methodological approach to superoxide dismutase (SOD) activity assay based on inhibition of nitroblue tetrazolium (NBT) reduction. Clin Chim Acta 1993; 214: 103-4
  13. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70: 158-70
  14. Şahin Ü, Tahan V, Akkaya A. Primer akciğer kanserlerinde lipid peroksidasyonu ve eritrosit antioksidan enzim aktivitesi. Tüberküloz veToraks 1999; 47: 31-3
  15. Petruzzelli S, Hietanen E, Barstch H. Lipid peroxidation in cigarette smokers and lung cancer patients. Chest 1990; 98: 930- 5
  16. Demircin G, Öner A. Serbest radikaller, reaktif oksijen molekülleri ve oksijen hasarı. Klinik Bilimler 1998; 4: 439-45
  17. Nadeem A, Chhabra SK, Masood A, Raj HGJ. Increased oxidative stres and altered levels of antioxidants in asthma. Allergy Clin Immunol 1990; 1 1 1: 72-8
  18. Wood LG, Fitzg