Journals / Su Ürünleri Dergisi / 2018 / Cilt: 35 - Sayı: 2

The effect of different growth medium and different light path length on the culture of Chlorella vulgaris

Farklı besin ortamının ve farklı ışık yolu uzunluğunun Chlorella vulgaris kültüründe etkisi

Pages
169–174
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Moreover, the inversely proportion between growth rates and the chlorophyll-a values was observed.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, the effects of panel photobioreactors with different nutrient mediums and different light path lengths on Chlorella vulgaris cultures were investigated.
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Key Findings & Data:Rapid cell growth was detected in the Jaworski nutrient medium (60.8 x 106 cells mL-1 ).
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Conclusion & Impact:Moreover, the inversely proportion between growth rates and the chlorophyll-a values was observed.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, the effects of panel photobioreactors with different nutrient mediums and different light path lengths on Chlorella vulgaris cultures were investigated. Rapid cell growth was detected in the Jaworski nutrient medium (60.8 x 106 cells mL-1 ). In the F / 2 nutrient medium, the cell number was recorded as 1.37 x 106 cells mL-1 on the 11th day. Dry weight of cultures grown in Jaworski and F / 2 nutrient media were measured as 0.46 gL-1 and 0.13 gL-1 at the end of 11 days, respectively. At the experiments in glass panel bioreactor, the highest cell number was found on the 31st day (230 x 106 cells mL-1 ) in a bioreactor with 1 cm light path length. Whereas, the highest cell numbers was found as 90 x 106 cells mL-1 in glass panel bioreactors with 3 cm and 5 cm light path length. The maximum dry weights for bioreactors with 1, 3 and 5 cm light path lengths were determined as 3.6 gL-1 , 2.8 gL-1 and 2.4 gL-1 , respectively. It was also determined that chlorophyll a values of Chlorella vulgaris on the panels bioreactors decreased over time. Moreover, the inversely proportion between growth rates and the chlorophyll-a values was observed.

Özet

Bu araştırmada Chlorella vulgaris kültürlerinde farklı ortamların ve farklı ışık yolu uzunluğuna sahip olan panel fotobiyoreaktörlerin etkileri incelenmiştir. Jaworski besin ortamında hızlı bir hücre artışı tespit edilmiştir (60,8 x 106 hücre mL-1 ). F/2 besin ortamında ise 11. günde hücre sayısı 1,37 x 106 hücre mL-1 olarak kayıt edilmiştir. Jaworski ve F/2 besin ortamlarında yetişen kültürlerin kuru ağırlık değeri 11. gün sonunda 0,46 gL-1 , 0,13 gL-1 olarak ölçülmüştür. Cam panel biyoreaktör denemelerinde en yüksek hücre sayısına 31. günde 1 cm ışık yolu uzunluğuna sahip biyoreaktörde ulaşılmıştır (230 x 106 hücre mL-1 ). Fakat 3 cm ve 5cm ışık yoluna sahip biyoreaktörlerde ise en yüksek hücre sayısı olduğu değer 90 x 106 hücre mL-1 olarak tespit edilmiştir. 1, 3 ve 5 cm ışık yolu uzunluğuna sahip biyoreaktörlerde maksimum kuru ağırlık değerleri ise sırasıyla 3,6 gL-1 , 2,8 gL-1 ve 2,4 gL-1 olarak tespit edilmiştir. Panellerde yapılan Chlorella vulgaris kültürlerin klorofil a değerleri zamanla düştüğü tespit edilmiştir. Aynı zamanda yapılan denemede büyüme oranları ile klorofil a değerlerinin ters orantılı olduğu gözlenmiştir.

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Cite This Article

DURMAZ, Y., TEMLİ, G. (2018). The effect of different growth medium and different light path length on the culture of Chlorella vulgaris. Su Ürünleri Dergisi, Cilt: 35 - Sayı: 2, 169–174. https://ajindex.com/articles/311135

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References (7)

  1. Cohen, Z., Vonshak, A. & Richmond, A. (1988). Effect of environmental conditions on fatty acid composition of the red alga Porphyridium cruentum: correlation to growth rate. Journal of Phycology, 24(3), 328- 332. DOI: 10.1111/j.1529-8817.1988.tb04474.x Brown, M.R., Jeffrey, S.W. & Garland, C.D. (1989). Nutritional aspects of microalgae used in mariculture: a literature review. CSIRO Marine Laboratories press 0725-4598, no: 205. Richmond, A. (1986).
  2. Handbook Of Microalgal Mass Culture, FL: CRC Press, Boca Raton. Sánchez, M. D., Mantell, C., Rodrıguez, M., de La Ossa, E. M., Lubián, L. M., & Montero, O. (2005). Supercritical fluid extraction of carotenoids and chlorophyll a from Nannochloropsis gaditana, Journal of Food Engineering, 66(2), 245-251. DOI: 10.1016/j.jfoodeng.2004.03.021 Sukenik, A. (1991). Ecophysiological considerations in the optimization of eicosapentaenoic acid production by Nannochloropsis sp. (Eustigmatophyceae). Bioresource Technology, 35, 263–269. DOI: 10.1016/0960-8524(91)90123-2
  3. Tompkins, J., Deville, M. M., Day, J. G. & Turner, M.F. (1995). The culture collection of algae and protozoa. Ambleside: Institute of Freshwater Ecology, Culture collection of algae and protozoa. Catalogue of strains; p. 204. Pulz, O. (2001). Photobioreaktors; Production Systems For Phototrophic Microorganisms. Igv Institute For Cereal Processing, Arthur- ShcheunerdAlle, Bergholz- Rehbrücke, 40/41: Germany. Guillard, R.R.L. (1975). Culture of phytoplankton for feeding marine invertebrate. In: Smith, W.L., Chanley, M.H. (Eds.), Culture of Marine Invertebrates Animals. Plenum, New York. Durmaz, Y & Erbil, G.Ç. (2017). Performance of industrial-scale tubular photobioreactor in marine hatchery. Journal of Applied Phycolgy. DOI: 10.1007/s10811-017-1202-7
  4. Uslu, L., Ak, B., Işık, O & Durmaz, Y. (2014) Effect of light path length and nitrogen deficiency on the biochemical composition of Phaeodactylum tricornutum. Fresenius Environmental Bulletin, 23(6), 1309-1313.
  5. Zar, J.H. (1999). Biostatistical Analysis. Upper Saddle River, Prentice Hall, New Jersey. 4th Edition. cap 12, USA. 231-272.
  6. Zou, N. & Richmond, A. (1999). Effect of light- path length in outdoor flate plate reactors on output rate of cell mass and of EPA in Nannochloropsis sp, Journal of Biotechnology, 70, 351-356. DOI: 10.1016/S0168-1656(99)00087-5
  7. Durmaz, Y. (2000). Chlorella sp’ nin ince cam panel biyoreaktörlerde üretiminde ışığın etkisi üzerine bir araştırma, Ege Üniversitesi Fen Bilimleri Enstitüsü Yü