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Journals / Su Ürünleri Dergisi / 2016 / Cilt: 33 - Sayı: 2

Electrophoretic comparison of vitellogenin protein in some fish species in Mogan Lake

Mogan Gölü’ndeki bazı balık türlerinde vitellogenin proteininin elektroforetik karşılaştırılması

GÖKTUĞ GÜL
Aysel Çağlan KARASU BENLİ
Figen ERKOÇ
Pages
151–156
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to results of our investigation, it was understood that this method is one of the effective bio-indicators.

📌 3 Key Takeaways

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Objective & Hypothesis:This study was carried out between January and May 2013 in Mogan Lake (Ankara - Turkey) which is used as fisheries and recreation area.
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Key Findings & Data:The Vitellogenin (VTG) protein was investigated by the electrophoretic SDS-Page (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) method from the blood plasma of female and male members of Cyprinus carpio, Carassius gibelio, Tinca tinca and Esox lucius species living in the lake and VTG proteins have been compared.
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Conclusion & Impact:According to results of our investigation, it was understood that this method is one of the effective bio-indicators.
🔬Design:Research Article
📍Scope:2013 Verileri
⚙️Variables:Domain Parameters

Abstract

This study was carried out between January and May 2013 in Mogan Lake (Ankara - Turkey) which is used as fisheries and recreation area. The Vitellogenin (VTG) protein was investigated by the electrophoretic SDS-Page (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) method from the blood plasma of female and male members of Cyprinus carpio, Carassius gibelio, Tinca tinca and Esox lucius species living in the lake and VTG proteins have been compared. In this method, Biometra brand vertical electrophoresis device has been used. During the breeding period, VTG proteins have been determined among male members of C. carpio, T. tinca, and E. lucius. It is taught that our results might be affected by various contaminants found in the lake. According to results of our investigation, it was understood that this method is one of the effective bio-indicators.

Özet

Bu çalışma, balıkçılık ve rekreasyon alanı olarak kullanılan Mogan Gölü'nde (Ankara - Türkiye) Ocak-Mayıs 2013 ayları arasında yürütülmüştür. Gölde yaşayan balık türlerinden Cyprinus carpio, Carassius gibelio, Tinca tinca ve Esox lucius türlerinin erkek ve dişi bireylerinin kan plazmasında elektroforetik SDS-Page (Sodyum Dodesil Sülfat Poliakrilamid Jel Elektroforezi) metodu ile vitellogenin (VTG) proteini araştırılmış ve türler arası karşılaştırma yapılmıştır. Bu metot için Biometra marka dikey elektroforez cihazı kullanılmıştır. Üreme döneminde C. carpio, T. tinca ve E. Lucius'un erkek bireylerinde vitellogenin proteini saptanmıştır. Göl ortamındaki çeşitli kirleticilerin bu sonuçlar üzerinde etkili olduğu düşünülmektedir. Bu çalışmada elde edilen bulgulara göre kullanılan testin iyi bir biyo-belirteç olduğu anlaşılmıştır

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Cite This Article

GÜL, G., BENLİ, A. Ç. K., ERKOÇ, F. (2016). Electrophoretic comparison of vitellogenin protein in some fish species in Mogan Lake. Su Ürünleri Dergisi, Cilt: 33 - Sayı: 2, 151–156. https://ajindex.com/articles/311101

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References (6)

  1. Allner, B., Wegener, G., Knacker, T. & Stahlschmidt-Allner, P. (1999). Electrophoretic determination of estrogen-induced protein in fish exposed to synthetic and naturally occurring chemicals. The Science of the Total Environment, 233(1-3): 21-31. doi:10.1016/S0048-9697(99)00176-X Asturiano, J.F., Romaguera, F., Aragon, P., Atienza, J., Puchades, R. & Maquieira, A. (2005). Sandwich immunoassay for determination of vitellogenin in golden grey mullet (Liza aurata) serum as a field exposure biomarker. Analytical and Bioanalytical Chemistry, 381: 1152-1160. doi:10.1007/s00216-004-3049-4
  2. Bjorkblom, C., Mustamaki, N., Olsson, P., Katsiadaki, I. & Wiklund, T., (2011). Assessment of reproductive biomarkers in three-spined stickleback (Gasterosteus aculeatus) from sewage effluent recipients. Environmental Toxicology, 28: 229–237. doi: 10.1002/tox.20715 Brion, F., Rogerieux, F., Noury, P., Migeon, B., Flammarion, P., Thybaud, E. & Porcher, J.M. (2000). Two-step purification method of vitellogenin from three teleost fish species: rainbow trout (Oncorhynchus mykiss), gudgeon (Gobio gobio) and chub (Leuciscus cephalus). Journal of Chromatography. B, Biomedical Sciences and Applications, 737(1-2): 3- 12. doi:10.1016/S0378-4347(99)00406-5
  3. Colborn, T., vom Saal, F.S. & Soto, A. M. (1993). Developmental effects of endocrine-disrupting chemicals in wildlife and humans. Environmental Health Perspectives, 101(5): 378-384. doi:10.2307/3431890 Coldham, N.G., Mehul, D., Sivapathasundaram, S., McDonnell, D.P., Conor, C. & Sauer, M.J. (1997). Evaluation of a recombinant yeast cell estrogen screening assay. Environmental Health Perspectives, 105: 734-742. doi:10.2307/3433729 Çek, Ş. & Sarıhan, F. (2010). The Effects of Sex Steroids of Endocrine Disrupting Chemicals on Fishes (in Turkish with English abstract). Ege Journal of Fisheries and Aquatic Sciences, 27(1): 41-46.
  4. Donohoe, R.M. & Curtis, L.R. (1996). Estrogenic activity of chlordecone, o,p’- DDT and o,p’-DDE in juvenile rainbow trout: induction of vitellogenesis and interaction with hepatic estrogen binding sites. Aquatic Toxicology, 36: 31- 52. doi:10.1016/S0166-445X(96)00799-0
  5. Fakıoğlu, Ö. & Polatsü, S. (2005). Determination of external phosphorus loading in Mogan Lake (Ankara) following some restoration measures (in Turkish with English abstract) Yuzuncu Yil University Journal of Agricultural Sciences, 15(1): 63-69.
  6. Fenech, M., (2000). The in vitro micronucleus technique. Mutation Research, 455, 81-95. doi:10.1016/S0027-51