SavedAdd your journal

Journals / Su Ürünleri Dergisi / 2017 / Cilt: 34 - Sayı: 2

Effects of mercury on some enzymes in the gill and muscle tissues ofOreochromis niloticus (Nile tilapia)

Oreochromis niloticus'un (Nil Tilapyası) solungaç ve kas dokularında civanın bazı enzimlere etkisi

GÜLBİN FİRİDİN
Pages
133–137
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The data obtained revealed that AChE, GPx and GPT activities in the gill and muscle tissues of Oreochromis niloticus were observed to change as a result of the exposures of both low and high mercury concentrations.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The toxic effects of the metals on the aquatic ecosystems can be found by measuring enzyme activities of fish tissues.
📊
Key Findings & Data:This study aimed to analyze the results of mercury (Hg) exposure on the change of the asetylcolinestrase (AChE), glutation peroxidase (GPx) and glutamic piruvate transaminase (GPT) activities in gills and muscles of Oreochromis niloticus (Linnaeus, 1758).
💡
Conclusion & Impact:The data obtained revealed that AChE, GPx and GPT activities in the gill and muscle tissues of Oreochromis niloticus were observed to change as a result of the exposures of both low and high mercury concentrations.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The toxic effects of the metals on the aquatic ecosystems can be found by measuring enzyme activities of fish tissues. This study aimed to analyze the results of mercury (Hg) exposure on the change of the asetylcolinestrase (AChE), glutation peroxidase (GPx) and glutamic piruvate transaminase (GPT) activities in gills and muscles of Oreochromis niloticus (Linnaeus, 1758). The fish were exposed to 0.1 and 0.01 ppm Hg for periods of 7 and 21 days. The enzyme activities in the tissues were determined by the use of ultraviolet spectroscopy. The AChE activity decreased while the GPx activity increased in both muscle and gills as a result to exposure to Hg in experimental group when compared to the control group. The GPT activity in gills were found to increase in the first 7 days of exposure and decreased after the exposure of 21 days. The data obtained revealed that AChE, GPx and GPT activities in the gill and muscle tissues of Oreochromis niloticus were observed to change as a result of the exposures of both low and high mercury concentrations.

Özet

Akuatik ekosistemler üzerine metallerin toksik etkileri balıklarda enzim aktivitelerinin ölçülmesiyle değerlendirilebilmektedir. Bu çalışmada civanın (Hg) Oreochromis niloticus (Linnaeus, 1758)'un solungaç ve kas dokularında asetilkolinestraz (AChE), glutatyon peroksidaz (GPx) ve glutamik piruvat transaminaz (GPT) aktivitelerine etkisi incelenmiştir. Balıklar 7 ve 21 günlük sürelerle 0,1 ve 0,01 ppm Hg konsantrasyonlarının etkisine maruz bırakılmıştır. Dokuların enzim aktiviteleri ultraviyole spektrofotometrik yöntem ile belirlenmiştir. Civa etkisinde AChE aktivitesi kontrol grubuna göre solungaç ve kas dokularında genel olarak azalırken GPx aktivitesi artmıştır. Kas GPT aktivitesi her iki sürede de kontrol grubuna göre artmıştır. Solungaçta GPT aktivitesi ise ilk 7 günlük sürede artarken 21 günlük sürede azalmıştır. Araştırma sonuçları düşük ve yüksek civa konsantrayonlarının Oreochromis niloticus'un solungaç ve kas dokularında AChE, GPx ve GPT aktivitelerinin değişmesine neden olduğunu göstermiştir.

🔖

Cite This Article

FİRİDİN, G. (2017). Effects of mercury on some enzymes in the gill and muscle tissues ofOreochromis niloticus (Nile tilapia). Su Ürünleri Dergisi, Cilt: 34 - Sayı: 2, 133–137. https://ajindex.com/articles/311059

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (6)

  1. Alves, L.M., Lemos, M.F.L., Correiaa, J.P.S., Costaa, N.A.R. & Novais S.C. (2015). The potential of cholinesterases as tools for biomonitoring studies with sharks: Biochemical characterization in brain and muscle tissues of Prionace glauca.Journal of Experimental Marine Biology and Ecology, 465: 49-55. doi: 10.1016/j.jembe.2015.01.006 Badiou, A. & Belzunces, L.P. (2008). Is acetylcholinesterase a pertinent biomarker to detect exposure of pyrethroids? A study case with deltamethrin. Chemico-Biological Interactions, 25: 175(1-3):406-409. doi: 10.1016/j.cbi.2008.05.040 Berntssen, M.H.G, Aatland, A. & Handy, R.D. (2003). Chronic dietary mercury exposure causes oxidative stress, brain lesions, and altered behaviour in Atlantic salmon (Salmo salar). Aquatic Toxicology, 65: 55-72. doi: /10.1016/S0166-445X(03)00104-8
  2. Beutler, E. (1984). Red cell metabolism: A manual of biochemical methods, 2nd ed., New York, Grune and Stratton. Branco, V., Canario, J., Lu, J., Holmgren, A. & Carvalho, C. (2012). Mercury and selenium interaction in vivo: Effects on thioredoxin reductase and glutathione peroxidase. Free Radical Biology and Medicine, 52: 781-793. doi:10.1016/j.freeradbiomed.2011.12.002 Chen, C.Y., Wooster, G.S., Getchell, R.G., Bowser, P.R. & Timmons, M.B. (2003). Blood chemistry of healthy, nephrocalcinosis-affected and ozonetreated tilapia in a recirculation system, with application of discriminant analysis. Aquaculture, 218: 89-102. doi:10.1016/S0044-8486(02)00499-4
  3. Costa, J.R.M., Mela, M., Assis, H.C.S., Pelletier, E., Randi, M.A.F. & OliveiraRibeiro, C.A. (2007). Enzymatic inhibition and morphological changes in Hoplias malabaricusfrom dietary exposure to lead (II) or methylmercury. Ecotoxicology and Environmental Safety, 67: 82-88.
  4. De La Torre, F., Salibian, A. & Ferrari, L. (1999). Enzyme Activities as Biomarkers of Freshwater Pollution: Responses of Fish Branchial (Na +K) ATPase and Liver Transaminases. Environmental Toxicology, 14: 313- 319. De La Torre, F., Salibian, A. & Ferrari, L. (2000). Biomarkers assessment in juvenile Cyprinus carpio exposed to waterborne cadmium. Environmental Pollution, 109: 277-282.
  5. Ellman, G.L., Courtney, K.D., Andres, V. & Featherstone, R.M. (1961). A new and rapid colorimetric determination of acetycholinesterase activity. Biochemical Pharmacology, 7: 88-95.
  6. Fırat, Ö. & Şahin İnandı, A. (2016). Investigation of combined effect of zeolite and mercury toxicity on some serum biochemical parameters of Oreochromis niloticus. Ege Jou