Journals / Van Veterinary Journal / 2015 / Cilt: 26 - Sayı: 3

Determination of Aflatoxin M1 Levels in Unpackaged Sold Raw Cow's Milk

Ambalajsız Olarak Satışa Sunulan Çiğ İnek Sütlerinde Aflatoksin M1 Düzeyinin Belirlenmesi

Pages
151–155
Publication Date
2015-12-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Therefore, should be established a good monitoring and control system in raw milk production and shall prevent the sale and consumption of milk containing high AFM1

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The purpose of this study was to determine the levels of aflatoxin M1 (AFM1) in unpackaged sold raw cow's milk in center of the Van province, Turkey.
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Key Findings & Data:For this purpose, 100 unpackaged raw cow's milk samples were collected from retail stores in center of the Van province during summer (June, July and August) and analyzed for presence and levels of AFM1.
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Conclusion & Impact:Therefore, should be established a good monitoring and control system in raw milk production and shall prevent the sale and consumption of milk containing high AFM1
🔬Design:Research Article
📍Scope:15 samples
⚙️Variables:Çiğ inek sütü · AFM1 · ELISA

Abstract

The purpose of this study was to determine the levels of aflatoxin M1 (AFM1) in unpackaged sold raw cow's milk in center of the Van province, Turkey. For this purpose, 100 unpackaged raw cow's milk samples were collected from retail stores in center of the Van province during summer (June, July and August) and analyzed for presence and levels of AFM1. The levels of AFM1 in the milk samples were determined using the enzyme linked immunoassay (ELISA). In 100 raw cow’s milk samples analyzed, where AFM1 is not found in 15 samples (15%), it is found; below detection limit (5 ppt) in 53 samples (53%), between 6.61 and 76.96 ng/l, average 24.60±17.89 ng/l in 21 samples (21%) and above 80 ng/l in 11 samples in (11%). It is found out that, 12 (12%) of raw cow’s milk samples aren’t compatible with the limits (50 ng/l) for milk in Turkish Food Codex in terms of aflatoxine M1 levels. Detecting aflatoxine M1 levels above legal limits in raw cow’s milk samples put up for sale indicate that consumption of these milks pose important risk in terms of public health. Therefore, should be established a good monitoring and control system in raw milk production and shall prevent the sale and consumption of milk containing high AFM1

Özet

Bu araştırma Van il merkezinde ambalajsız olarak satışa sunulan çiğ inek sütlerinde aflatoksin M1 (AFM1) düzeyini belirlemek amacıyla yapılmıştır. Bu amaçla yaz mevsiminde (haziran, temmuz ve ağustos aylarında) Van il merkezinde bulunan marketlerde ambalajsız olarak satışa sunulan 100 adet çiğ süt örneği aflatoksin M1varlığı ve seviyesi yönünden analize alınmıştır. Süt örneklerinde AFM1 varlığı ve düzeyi kompetetif ELISA (Enzyme-Linked Immunosorbent Assay) yöntemi ile tespit edilmiştir. Analize alınan 100 çiğ inek sütü örneğinin 15 tanesinde (%15) AFM1 belirlenememiş, 53 tanesinde (%53) tespit limitinin (5 ppt) altında bulunmuş, 21 tanesinde (%21) 6.61 ile 76.96 ng/l arasında ortalama 24.60±17.89 ng/l olarak bulunurken 11 (%11) örnekte ise 80 ng/l’den yüksek seviyede AFM1 tespit edilmiştir. Analize alınan çiğ inek sütü örneklerinin 12 tanesinin (%12) AFM1 seviyesi yönünden Türk Gıda Kodeksi’nde süt için verilen limitlere (50 ng/l) uygun olmadığı ortaya konmuştur. Tüketime sunulan çiğ inek sütü örneklerinde yasal limitlerden yüksek seviyede AFM1 tespit edilmesi, bu ürünlerin tüketilmesinin halk sağlığı yönünden ciddi bir risk oluşturabileceğini göstermektedir. Bu nedenle, çiğ süt üretiminde iyi bir izleme ve kontrol sistemi kurulmalı ve yüksek AFM1 içeren sütlerin satışının ve tüketiminin önüne geçilmelidir

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Cite This Article

İŞLEYİCİ, Ö., SANCAK, Y. C., SANCAK, H., YÜCEL, U. M. (2015). Determination of Aflatoxin M1 Levels in Unpackaged Sold Raw Cow's Milk. Van Veterinary Journal, Cilt: 26 - Sayı: 3, 151–155. https://ajindex.com/articles/30911

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References (13)

  1. Akdemir Ç, Altıntaş A (2004). Ankara’da işlenen sütlerde aflatoksin M1 varlığının ve düzeylerinin HPLC ile araştırılması. Ankara Üniv Vet Fak, 51, 175-179.
  2. Aljicevic M, Hamzic S (2010). Aflatoxin in the urine of patients suffering fom HCC and HBV. J of Society for Development in New Net Environment in B&H, 4(4), 852-856.
  3. Anonim (2008). Gıda Maddelerinde Belirli Bulaşanların Maksimum Seviyelerinin Belirlenmesi Hakkında Tebliğ. Resmi Gazete, 17 Mayıs 2008, sayı: 26879, Başbakanlık Basımevi, Ankara.
  4. Anonymous (1997). Worldwide regulations for mycotoxins 1995.
  5. A compendium FAO, Food and Nutrition Paper, 64, Rome. Anonymous (2001). Report of the 33rd Session of the Codex Committee on Food Additives and Contaminants. Joint FAO/WHO Food Standarts Programme Codex Alimentarius Commision. Twenty-Fourth Session, Geneva, Switzerland. Organisation of The United Nations. Anonymous (2002). Enzyme immunoassay for the quantitative analysis of aflatoxin M1 in milk and milk products. Immunolab GmbH, Darmstadt, 1-6,
  6. Germany. Anonymous (2006). Enzyme immunoassay for the quantitative analysis of aflatoxin M1. Art. No: R1111, Lot: 13400, R-Biopharm AG, Darmstadt, Germany. Anonymous (2006a). Enzyme immunoassay for the quantitative analysis of aflatoxin M1. Art. No: R1101, R-Biopharm AG, Darmstadt, Germany. Anonymous (2010). Regulations. Commission Regulation (EU) No: 165/2010. Amending Regulation (EC) No 1881/2006, setting maximum levels for certain contaminants in foodstuffs as regards aflatoxins. Official Journal of the European Union, L, 50, 8-12.
  7. Atasever M, Nizamlıoğlu M, Özturhan K, Karakaya Y, Ünsal C (2006). Erzurum bölgesinde tüketime sunulan süt ve süt ürünlerinin aflatoksin M1 yönünden incelenmesi. 2. Ulusal Veteriner Gıda Hijyeni Kongresi (Uluslar arası katılımlı) Bildiri Kitabı, sh:231-240, 18-20 Eylül 2006,
  8. İstanbul.
  9. Bakirci I (2001). A study on the occurence of aflatoxin M1 in milk and milk products produced in Van province of Turkey. Food Control, 12, 47-51.
  10. Barbieri G., Bergamini C, Ori E, Resca P (1994). Aflatoxin M1 in parmesan cheese: HPLC determination. J Food Sci, 59, 1313-1331.
  11. Boudra H, Barnoin J, Dragacci S, Morgavi DP (2007). Aflatoxin M1 and ochratoxin A in raw bulk milk from French dairy herds. J Dairy Sci, 90(7), 3197-3201.
  12. Budağ C, Keçeci Ş (2013). Van’da büyükbaş hayvan besilerinde kullanılan yemler ve besi şekillerine ilişkin bir anket çalışması. YYÜ Fen Bil Enst Derg, 18(1-2), 48-61.
  13. Buldu HM, Koç AN, Uraz G (2011). Aflatoxin M1 kontamination in c