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Journals / Mikrobiyoloji Bülteni / 2002 / Cilt: 36 - Sayı: 3

Comparison of BBL mycobacteria growth indicator tube (MGIT) method, Bactec radiometric system and Lowenstein-Jensen media for the detection of mycobacteria in clinical specimens

Klinik örneklerde mikobakterilerin saptanmasında BBL mikobakteri üreme indikatör tüp (MGIT), Bactec radyometrik sistemi ve Lowenstein-Jensen besiyerinin karşılaştırılması

ALPASLAN ALP
Gülşen HASÇELİK
Pages
229–235
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

As a result, it was found that the recovery of mycobacteria in MGIT system is comparable to BACTEC 460 system.

📌 3 Key Takeaways

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Objective & Hypothesis:SUMMARY: The need for more rapid diagnostic tests for early detection of mycobacteria in clinical specimens has become more apparent for the control of tuberculosis.
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Key Findings & Data:The aim of this study was to evaluate the Mycobacteria Growth Incubator Tube (MGIT), BACTEC 460 and Lowenstein-Jensen (LJ) media for the recovery of mycobacteria from processe clinical specimens.
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Conclusion & Impact:As a result, it was found that the recovery of mycobacteria in MGIT system is comparable to BACTEC 460 system.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Mycobacterium · Mycobacterium tuberculosis · Mycobacteriaceae

Abstract

SUMMARY: The need for more rapid diagnostic tests for early detection of mycobacteria in clinical specimens has become more apparent for the control of tuberculosis. Several commercial systems, as well as conventional media, are available for the culture of acid-fast bacteria (AFB). The aim of this study was to evaluate the Mycobacteria Growth Incubator Tube (MGIT), BACTEC 460 and Lowenstein-Jensen (LJ) media for the recovery of mycobacteria from processe clinical specimens. A total of 75 clinical specimens which were found positive for AFB in smear microscopy were included to the study, and of them 73 were grown in MGIT system, 70 in BACTEC 460 system and 74 on the LJ slants. Average periods for the detection of mycobacteria for BACTEC 460, MGIT systems and LJ medium were 7.3 days, 10.9 days and 19.4 days, respectively. As a result, it was found that the recovery of mycobacteria in MGIT system is comparable to BACTEC 460 system. It appears to be a safer system than BACTEC 460 system, and may be used for detection of mycobacteria in laboratories desiring a non-radioactive method.

Özet

ÖZET: Günümüzde tüberkülozun kontrol altına alınabilmesi amacıyla, klinik örneklerden mikobakterilerin izolasyonunda daha hızlı sonuç veren testlere duyulan ihtiyaç artmıştır. Aside dirençli basillerin kültür yöntemleri ile tanısında konvansiyonel kültür yöntemlerinin yanısıra, çeşitli ticari kültür sistemleri de kullanıma girmiştir. Bu çalışma, klinik örneklerden mikobakterilerin izole edilmesinde rutin kullanıma yeni girmiş olan MGIT (Mycobacteria Growth Indicator Tube) sistemi ile BACTEC 460 TB radyometrik sistemi ve Lowenstein-Jensen besiyerinin karşılaştırılması amacıyla yapılmıştır. Çalışmaya mikroskobik inceleme sonrasında aside dirençli basil saptanan 75 klinik örnek alınmıştır. İncelenen örneklerin 70'inde BACTEC 460 TB sistemi, 73'ünde MGIT sistemi, 74'ünde de Lowenstein-Jensen besiyeri ile mikobakteriyel üreme saptanmıştır. Üç kültür yöntemi ile mikobakteri saptanması için gereken ortalama süre sırasıyla, BACTEC 460 TB sisteminde 7.3 gün, MGIT sisteminde 10.9 gün ve Lowenstein-Jensen besiyerinde 19.4 gün olarak belirlenmiştir. Çalışmanın sonucunda MGIT yönteminin mikobakterilerin saptanmasında BACTEC sistemine oranla daha güvenli bir yöntem olduğu, özellikle radyoaktif elementlerle uğraşmak istemeyen laboratuvarlarda rutin kullanıma girebileceği ve BACTEC 460 TB sistemine alternatif bir yöntem olabileceği sonucuna varılmıştır.

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Cite This Article

ALP, A., HASÇELİK, G. (2002). Comparison of BBL mycobacteria growth indicator tube (MGIT) method, Bactec radiometric system and Lowenstein-Jensen media for the detection of mycobacteria in clinical specimens. Mikrobiyoloji Bülteni, Cilt: 36 - Sayı: 3, 229–235. https://ajindex.com/articles/299504

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References (2)

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