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Journals / Mikrobiyoloji Bülteni / 2003 / Cilt: 37 - Sayı: 1

In-vitro susceptibility of Salmonella enterica serovar typhimurium to reactive oxygen and nitrogen intermediates

Salmonella enterica serovar typhimurium'un reaktif oksijen ve nitrojen ara ürünlerine in-vitro duyarlılığı

AHMET YILMAZ ÇOBAN
BORA EKİNCİ
Belma DURUPINAR
Pages
27–33
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These data indicated thatfurther studies were necessary on the genetic regulatory systems in Salmonella which control the resistance against RNI and ROl-dependent antimicrobial systems.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to investigate the in-vitro susceptibility of Salmonella enterica serovar Typhimurium against reactive oxygen and nitrogen intermediates, on five different strains.
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Key Findings & Data:SH5014 as a parental strain, PP120 as a soxRS mutant, SH7616 as a acrAB efflux pump mutant, SZH KUEN 557 as a Standard strain and a clinical isolate, vvere included to the study.
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Conclusion & Impact:These data indicated thatfurther studies were necessary on the genetic regulatory systems in Salmonella which control the resistance against RNI and ROl-dependent antimicrobial systems.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Salmonella typhimurium · Triyazinler · Reaktif oksijen türleri

Abstract

The aim of this study was to investigate the in-vitro susceptibility of Salmonella enterica serovar Typhimurium against reactive oxygen and nitrogen intermediates, on five different strains. SH5014 as a parental strain, PP120 as a soxRS mutant, SH7616 as a acrAB efflux pump mutant, SZH KUEN 557 as a Standard strain and a clinical isolate, vvere included to the study. Hydrogen peroxide (at 0.125, 0.25, 0.5 mM concentrations) as reactive oxygen intermediate (ROI) and DETA-NO (at 1, 2, 4 mg/ml concentrations) as nitric oxide donor, in addition vvith acidified sodium nitrite (at 25, 50, 100 mM concentrations) as reactive nitrogen intermediate (RNI) were used. As a result, susceptibility of PP120 to RNI and ROI was found similar with SZH KUEN 557 and the clinical isolate, vvhile SH7616 and SH5014 vvere found more susceptible than the others. These data indicated thatfurther studies were necessary on the genetic regulatory systems in Salmonella which control the resistance against RNI and ROl-dependent antimicrobial systems.

Özet

Bu çalışmada, Salmonella enterica serovar Typhimurium'un reaktif oksijen ve reaktif nitrojen ara ürünlerine karşı in-vitro duyarlılığını araştırmak amacıyla, 5 farklı sus test edilmiştir. Çalışmaya, parental sus olarak SH5014, soxRS mutantı olan PP120, acrAB efluks pompa mutantı olan SH7616, standart sus olarak SZH KUEN 557 ve bir klinik izolat dahil edilmiştir. Reaktif oksijen ara ürünü (ROI) olarak hidrojen peroksit (0.125, 0.25, 0.5mM yoğunluklarda) ve nitrik oksit donörü olarak DETA-NO (1, 2, 4 mg/ml yoğunluklarda) ile reaktif nitrojen ara ürünü (RNI) olarak asidifiye sodyum nitrit (25, 50, 100mM yoğunluklarda) kullanılmıştır. Sonuç olarak, PP120'nin RNI ve ROl'ya duyarlılıkları SZH KUEN 557 ve klinik susun duyarlılıkları ile benzer bulunmuş, SH7616 ve SH5014'ün ise diğerlerine göre daha duyarlı olduğu belirlenmiştir. Bu bulgular, reaktif oksijen ve nitrojen ara ürünlerine bağımlı antimikrobiyal mekanizmalara karşı, Salmonella'da direnci kontrol eden genetik sistemlerin daha ayrıntılı araştırılmasının gerekli olduğunu ifade etmektedir.

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Cite This Article

ÇOBAN, A. Y., EKİNCİ, B., DURUPINAR, B. (2003). In-vitro susceptibility of Salmonella enterica serovar typhimurium to reactive oxygen and nitrogen intermediates. Mikrobiyoloji Bülteni, Cilt: 37 - Sayı: 1, 27–33. https://ajindex.com/articles/299442

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