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Journals / Mikrobiyoloji Bülteni / 2013 / Cilt: 47 - Sayı: 2

Investigation of the presence of new delhi metallo-beta-lactamase-1 (ndm-1) by pcr in carbapenem-resistant gram-negative isolates

Karbapeneme dirençli gram-negatif izolatlarda new delhi metallo-beta-laktamaz-1 (ndm-1) varlığının pcr ile araştırılması

Keramettin YANIK
Dilek EMİR
Cafer EROĞLU
Adil KARADAĞ
Akif Koray GÜNEY
Murat GÜNAYDIN
Pages
382–384
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Meanwhile special care should be given on rational antibiotic use and establishment of appropriate infection control policies to prevent the spread of NDM-1 producers and other potential resistant strains.

📌 3 Key Takeaways

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Objective & Hypothesis:Bacteria producing New Delhi metallo-beta-lactamase-1 (NDM-1) exhibit high level resistance to beta-lactams including carbapenems.
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Key Findings & Data:This broad-spectrum resistance limits treatment options for infections caused by NDM-1 producers.
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Conclusion & Impact:Meanwhile special care should be given on rational antibiotic use and establishment of appropriate infection control policies to prevent the spread of NDM-1 producers and other potential resistant strains.
🔬Design:Research Article
📍Scope:2012 Verileri
⚙️Variables:Domain Parameters

Abstract

Bacteria producing New Delhi metallo-beta-lactamase-1 (NDM-1) exhibit high level resistance to beta-lactams including carbapenems. This broad-spectrum resistance limits treatment options for infections caused by NDM-1 producers. NDM-1 was first isolated from an Indian patient in Sweden; since then, NDM-1 producing isolates have been identified in many countries including Turkey. In this study, we investigated the presence of NDM-1 by PCR method in various gram-negative isolates recovered from clinical specimens in tertiary care hospitals in Samsun, Turkey. A total of 210 carbapenem-resistant gramnegative isolates (132 Acinetobacter baumannii, 54 Pseudomonas aeruginosa, 5 Pseudomonas putida, 8 Enterobacter cloacae, 3 Enterobacter aerogenes, 3 Klebsiella pneumoniae, 2 Providencia rettgeri, 2 Escherichia coli and 1 Citrobacter freundii) were included in the study. Identification and antibiotic susceptibility testing of the isolates were performed by using Vitek-2 Compact (bioMerieux, France) and BD Phoenix (BD Diagnostic Systems, MD) automated systems. The results of antibiotic susceptibility testing were interpreted according to the CLSI recommendations. In our study, NDM-1 gene was not detected in any of the clinical isolates by PCR. There was only one case study that reported the presence of NDM-1 in clinical isolates from Turkey [Poirel L et al. Antimicrob Agents Chemother 2012;56:2784]. Our data, together with the others, indicated that the existence of NDM-1 in clinical isolates is not common in Turkey. However, since NDM-1 is a plasmid-encoded enzyme, there is always a risk of spread of this resistance through the bacterial strains in our country. Therefore, continuous surveillance and investigation of carbapenem-resistant isolates with resistance patterns suggestive of NDM-1 may enable to identify NDM-1 producing isolates. Meanwhile special care should be given on rational antibiotic use and establishment of appropriate infection control policies to prevent the spread of NDM-1 producers and other potential resistant strains.

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Cite This Article

YANIK, K., EMİR, D., EROĞLU, C., KARADAĞ, A., GÜNEY, A. K., GÜNAYDIN, M. (2013). Investigation of the presence of new delhi metallo-beta-lactamase-1 (ndm-1) by pcr in carbapenem-resistant gram-negative isolates. Mikrobiyoloji Bülteni, Cilt: 47 - Sayı: 2, 382–384. https://ajindex.com/articles/299322

References (10)

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