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Journals / Mikrobiyoloji Bülteni / 2016 / Cilt: 50 - Sayı: 1

Investigation of Human Herpesvirus-8 Seroprevalence in Blood Donors and HIV-Positive Patients Admitted to Ege University Medical School Hospital, Turkey

Ege Üniversitesi Tıp Fakültesi Hastanesine Başvuran Kan Donörlerinde ve HIV-Pozitif Hastalarda İnsan Herpesvirusu-8 Seroprevalansının Araştırılması

İMRE ALTUĞLU
Ayfer YOLCU
ZELİHA ASLI ÖCEK
Rüçhan YAZAN SERTÖZ
AYŞE DENİZ GÖKENGİN
Pages
104–111
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result, HHV-8 seroprevalence rate detected in our study is similar to the data of other studies performed in Turkey, as well as the rates reported from other European and Asian countries

📌 3 Key Takeaways

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Objective & Hypothesis:Human herpesvirus 8 (HHV-8), classifi ed in Herpesviridae family, is the etiological agent of Kaposi's sarcoma (KS), primary effusion lymphoma and multicentric Castleman's disease.
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Key Findings & Data:In contrast to the other herpesviruses, HHV-8 seroprevalence is low in general populations; however, the higher prevalence observed in individuals with immunodefi ciencies such as AIDS poses an increased risk for KS.
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Conclusion & Impact:As a result, HHV-8 seroprevalence rate detected in our study is similar to the data of other studies performed in Turkey, as well as the rates reported from other European and Asian countries
🔬Design:Research Article
📍Scope:2013 Verileri
⚙️Variables:Domain Parameters

Abstract

Human herpesvirus 8 (HHV-8), classifi ed in Herpesviridae family, is the etiological agent of Kaposi's sarcoma (KS), primary effusion lymphoma and multicentric Castleman's disease. In contrast to the other herpesviruses, HHV-8 seroprevalence is low in general populations; however, the higher prevalence observed in individuals with immunodefi ciencies such as AIDS poses an increased risk for KS. The global distribution of HHV-8 shows great variations, with the highest seroprevalence seen in Africa. The number of studies on the seroprevalence of HHV-8 in Turkey are limited. The aim of this study was to determine the HHV-8 seroprevalences in healthy blood donors and HIV-positive patients, that will contribute HHV-8 seroepidemiological data in our country. This study was designed as a cross-sectional study. A total of 551 healthy donors (76 female, 475 male; age range: 18-65 years) admitted to Ege University Medical School Hospital, Blood Center for blood donation between December 2013-January 2014, and 173 HIV-positive patients (30 female, 143 male; age range: 18-65 years) admitted to infectious diseases outpatient clinic between October 2013-January 2014, were included in the study. A commercial ELISA method (KSHV/HHV-8 IgG ELISA Kit, Advanced Biotechnologies Inc, USA) was used for the detection of IgG antibodies that were structured against HHV-8 lytic antigens. In the study, 29 (29/551, 5.3%) of blood donors and 44 (44/173, 25.4%) of HIV-positive patients, with a total of 73 (73/724, 10.1%) cases were found as HHV-8 seropositive. The difference between blood donors and HIV-positive patients in terms of HHV-8 seropositivity rates was statistically signifi cant (5.3% versus 25.4%; p< 0.05). In both of the study groups, no statistically signifi cant difference was detected between HHV-8 seropositivity with gender and age. When considering HIV-positive patients, no statistically signifi cant difference was observed between HHV-8 seropositivity with the duration of anti-HIV positivity, CD4+ T cell count, HIV-RNA status and history of having sexually transmitted disease. As a result, HHV-8 seroprevalence rate detected in our study is similar to the data of other studies performed in Turkey, as well as the rates reported from other European and Asian countries

Özet

Herpesviridae ailesinde yer alan insan herpesvirusu-8 (HHV-8), Kaposi sarkomu (KS), primer efüzyonlu lenfoma ve multisentrik Castleman hastalığının başlıca nedenidir. Diğer herpesvirusların aksine HHV-8 seroprevalansı genel popülasyonlarda düşüktür; ancak AIDS gibi immün yetmezliği olan kişilerde yüksek olan prevalans, KS gelişimi açısından risk oluşturmaktadır. HHV-8'in küresel yayılımı büyük değişkenlik göstermekte ve en yüksek seroprevalans Afrika'da izlenmektedir. Ülkemizde HHV-8 seroprevalansının araştırıldığı çalışma sayısı oldukça kısıtlıdır. Bu çalışmada, sağlıklı kan donörleri ile HIV-pozitif hastalarda HHV-8 seroprevalansının araştırılması ve ülkemizdeki HHV-8 seroepidemiyolojisine katkıda bulunulması amaçlanmıştır. Kesitsel olarak planlanan bu çalışmaya, Ege Üniversitesi Tıp Fakültesi Hastanesi Kan Merkezine Aralık 2013-Ocak 2014 tarihleri arasında gönüllü kan bağışı için başvuran 551 sağlıklı donör (76 kadın, 475 erkek; yaş aralığı: 18-65 yıl) ve Enfeksiyon Hastalıkları polikliniğine Ekim 2013-Ocak 2014 tarihleri arasında başvuran 173 HIV-pozitif hasta (30 kadın, 143 erkek; yaş aralığı: 18-65 yıl) dahil edilmiştir. Serum örneklerinde HHV-8 litik antijenlerine karşı gelişmiş IgG antikorlarını saptayan ticari bir ELISA kiti (KSHV/HHV-8 IgG ELISA Kit, Advanced Biotechnologies Inc, ABD) kullanılmıştır. Çalışmada, kan donörlerinin 29'unda (29/551, %5.3), HIV-pozitif hastaların ise 44'ünde (44/173, %25.4) olmak üzere, olguların toplam 73'ünde (73/724, %10.1) HHV-8 seropozitifl iği saptanmıştır. Kan donörleri ve HIV-pozitif hastalar arasında HHV-8 seropozitifl iği oranları arasındaki fark istatistiksel olarak anlamlı bulunmuştur (%5.3'e karşı %25.4; p 0.05). HIV-pozitif hastalarda; anti-HIV pozitifl ik süresi, CD4+ T hücre sayısı, HIV-RNA yükü ve cinsel yolla bulaşan diğer hastalık varlığı öyküsü ile HHV-8 seropozitifl iği arasında istatistiksel olarak anlamlı bir ilişki bulunmamıştır. Sonuç olarak, çalışmamızda saptanan HHV-8 seroprevalansının, ülkemizde yapılan diğer çalışmalar ve Avrupa ve Asya ülkelerinde saptanan seropozitifl ik oranları ile benzerlik gösterdiği belirlenmiştir

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Cite This Article

ALTUĞLU, İ., YOLCU, A., ÖCEK, Z. A., SERTÖZ, R. Y., GÖKENGİN, A. D. (2016). Investigation of Human Herpesvirus-8 Seroprevalence in Blood Donors and HIV-Positive Patients Admitted to Ege University Medical School Hospital, Turkey. Mikrobiyoloji Bülteni, Cilt: 50 - Sayı: 1, 104–111. https://ajindex.com/articles/298427

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