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Journals / Okmeydanı Tıp Dergisi / 2017 / Cilt: 33 - Sayı: 4

The Effect of Ghrelin Treatment on Cell Survival and Inflammation in Type 2 Diabetic Rat Liver

Tip 2 Diyabetik Sıçan Karaciğerinde Ghrelin Tedavisinin Hücre Sağkalımı ve İnflamasyonu Üzerine Etkileri

ZEYNEP MİNE COŞKUN
Alisa Bahar BEYDOGAN
Sema BOLKENT
Pages
253–260
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

However, this treatment did not adequately affect oxidative stress in diabetic rats.

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Objective & Hypothesis:Objective: The aim of the study is to investigate the effect of ghrelin treatment on regulation of cell survival, inflammation and oxidative stress in the rat liver of type-2 diabetes model.Materials and Methods: Twenty-one male Sprague-Dawley rats (8-10 weeks old) were divided into three groups: control, type-2 diabetes (T2D) and T2D+Ghrelin (25 ?g/kg with intraperitoneal (i.p) injection for two weeks).
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Key Findings & Data:Type-2 diabetes was induced by feeding 10% fructose solution in drinking water for 2 weeks and followed by a single i.p injection of streptozotocin (40 mg/kg).
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Conclusion & Impact:However, this treatment did not adequately affect oxidative stress in diabetic rats.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of the study is to investigate the effect of ghrelin treatment on regulation of cell survival, inflammation and oxidative stress in the rat liver of type-2 diabetes model.Materials and Methods: Twenty-one male Sprague-Dawley rats (8-10 weeks old) were divided into three groups: control, type-2 diabetes (T2D) and T2D+Ghrelin (25 ?g/kg with intraperitoneal (i.p) injection for two weeks). Type-2 diabetes was induced by feeding 10% fructose solution in drinking water for 2 weeks and followed by a single i.p injection of streptozotocin (40 mg/kg). Control animals received tap water. The liver samples were obtained from rats at the end of experiment. Glutathione (GSH), lipid peroxidation (LPO) and protein carbonyl (PCO) levels were measured in liver tissue. Matrix metalloproteinases (MMP-2 and -9), nuclear factor kappa B (NF-?B), peroxisome proliferatoractivated receptor gamma (PPAR-?), interleukin 6 (IL-6) and proliferating cell nuclear antigen (PCNA) expressions were determined by immunohistochemical methods.Results: The number of MMP-2, MMP-9, NF-?B, PPAR-? and IL-6 immunopositive cells increased in the diabetic rat liver as compared to control. The ghrelin treatment significantly reduced the numbers of MMP-2, MMP-9, NF?B, PPAR-? and IL-6 immunopositive cells in diabetic rat liver. However, the number of PCNA immunopositive cells increased in diabetic rats treated with ghrelin. Therapeutic effect of ghrelin was not shown in terms of the biochemical parameters including GSH and LPO but PCO levels decreased in the liver.Conclusions: According to our findings, ghrelin treatment could prevent diabetes-induced inflammation in the liver. However, this treatment did not adequately affect oxidative stress in diabetic rats.

Özet

Amaç: Çalışmamızın amacı, tip 2 diyabet modeli sıçan karaciğerlerinde ghrelin uygulamasının hücre sağkalımı, inflamasyon ve oksidatif stres düzenlenmesi üzerine etkilerinin incelenmesidir.Gereç ve Yöntemler: 21 adet Sprague-Dawley sıçanlar (8-10 haftalık) üç gruba ayrıldı: Kontrol, tip-2 diyabet (T2D) ve T2D+Ghrelin (25 ?g/kg iki hafta boyunca intraperitoneal (i.p.) olarak enjekte edildi). Tip-2 diyabet %10 fruktoz solüsyonunun iki hafta boyunca içme suyuna katılmasını takiben tek doz i.p. streptozotosin (40 mg/kg) enjekte edilmesi ile oluşturuldu. Kontrol hayvanlara çeşme suyu verildi. Deney sonunda karaciğer doku örnekleri alındı. Glutatyon (GSH), lipid peroksidasyonu (LPO) ve protein karbonil (PCO) seviyeleri karaciğer dokusunda ölçüldü. Matriks metalloproteinaz (MMP-2 ve MMP-9), nuklear faktör kappaB (NF-?B), peroksizom proliferatör-aktive reseptör gama (PPAR-?), interlökin 6 (IL-6), ve prolifere hücre nukleus antijeni (PCNA) ekspresyonları immunohistokimyasal metod ile belirlendi.Bulgular: MMP-2, MMP-9, NF-?B, PPAR-? ve IL-6 immünpozitif hücre sayısı diyabetik sıçan karaciğerinde kontrole göre arttı. Ghrelin tedavisi diyabetik sıçan karaciğerindeki MMP-2, MMP-9, NF-?B, PPAR-? ve IL-6 immünpozitif hücre sayılarını önemli derecede azalttı. Fakat ghrelin uygulanan diyabetiklerde PCNA immünpozitif hücre sayısı arttı. Sıçan karaciğerinde ghrelinin tedavi edici etkisi GSH ve LPO parametreleri için anlamlı bulunmadı fakat PCO seviyesi azaldı.Sonuç: Bulgularımıza göre, ghrelin tedavisi karaciğerdeki diyabetin neden olduğu inflamasyonu önleyebilir. Fakat bu tedavi diyabetik sıçanlarda oksidatif stresi yeterince etkilemedi.

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Cite This Article

COŞKUN, Z. M., BEYDOGAN, A. B., BOLKENT, S. (2017). The Effect of Ghrelin Treatment on Cell Survival and Inflammation in Type 2 Diabetic Rat Liver. Okmeydanı Tıp Dergisi, Cilt: 33 - Sayı: 4, 253–260. https://ajindex.com/articles/291886

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