AI Summary & Key Takeaways
AI InsightHyperbarik room air groups showed no significant changes in lipid peroxidation, when compared with control animals (p>0.05) CONCLUSION: It was concluded that the ambient air pressure have no meaningful effect on oxidative stress, which seems to be caused by HBO2 exposure.
📌 3 Key Takeaways
Abstract
PURPOSE: It is a known fact that hyperbaric oxygen (HBO2) exposure cause increase in free radical production. In this study, it was aimed to investigate whether the ambient air pressure have any role on a probable increased oxidative stress caused by HBO2. MATERIAL AND METHODS: 40 Sprague-Dawley (SD) rats were divided into 5 equal groups; (A) - control, (B and D) - groups exposed to HBO2 or room air at 3 ATA for 2 hours oncely, and (C and E) - another two group which got HBO2 or room air at 2 ATA for 90 minute long 10 seances (two seances/day). After the exposing procedure lipid peroxidation (TBARS), superoxide dismutase (SOD) and catalase (CAT) activity was measured in rats lung. RESULTS: If compared with controls, CAT activity was found to be increased significantly in both, HBO2 and also room air exposed groups for one seance (p<0,05). SOD activity incresed only in the chronic HBO2 group (p<0.05 when compared with controls). One seance HBO2 enhanced lipid peroxidation products significantly. This increase found also to be significant if compared with one seance room air and chronic HBO2 groups (p<0.05). Hyperbarik room air groups showed no significant changes in lipid peroxidation, when compared with control animals (p>0.05) CONCLUSION: It was concluded that the ambient air pressure have no meaningful effect on oxidative stress, which seems to be caused by HBO2 exposure.
Özet
AMAÇ: Hiperbarik oksijen (HBO2) uygulamaları sonucu serbest radikal üretiminin arttığı bilinen bir gerçektir. Bu çalışmada, HBO2 sonucu oluşabilecek olası bir oksidatif streste, maruz kalınan yüksek çevre basıncının da bunda rolü olup olmadığı konusuna açıklık getirilmeye çalışıldı. GEREÇ VE YÖNTEM: 40 adet Sprague-Dawley (SD) sıçan toplam 5 gruba ayrıldı. Bunlar; (A) - kontrol, (B) - 3 ATA'da 2 saat süreli tek seans HBO2, (C) - 2 ATA'da 1,5 saat süreli 10 seans (12 saat ara ile) HBO2, (D) - yine 3 ATA'da 2 saat süreli tek seans hiperbarik hava ve (E) - 2 ATA'da 1,5 saat süreli 10 seans hiperbarik hava şeklinde belirlendi. Prosedür sonunda sıçanlarda akciğer lipid peroksidasyonu (TBARS) ile süperoksit dismutaz (SOD) ve katalaz (CAT) enzim aktiviteleri ölçüldü. BULGULAR: CAT aktivitesi, hem tek seans HBO2 uygulanan grupta, hem de aynı doz ve sürede normal hava uygulanan grupta kontrol grubuna göre anlamlı olarak artmış bulundu (p 0.05). SONUÇ: HBO2'nin oluşturmuş göründüğü oksidatif streste hiperbarik ortamın hemen hemen hiç bir katkısı olmadığı kanaatine varıldı.