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Journals / Medical Network Klinik Bilimler ve Doktor / 2005 / Cilt: 11 - Sayı: 6

The effect of cumulus cell culture in the fragmantation formation of human embriyos

İnsan embriyolarında fragmantasyon oluşumuna karşı kumulus hücre kültürü etkisi

CEM KORKMAZ
MEHMET CINCIK
Münire AKAR
FÜSUN BELGİN SELAM
BARIŞ BAYKAL
Pages
641–647
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Cumulus cell coculture can be used as a practical and easy method in patients for decreasing the percentage of fragmantation .

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Our aim was to investigate the effect of cumulus cell culture on fragmantation formation in human embriyos.
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Key Findings & Data:Following controlled ovarian hyperstimulation with rec FSH and pituitary desensitization with GnRH analogues, 344 mature oocytes were obtained and ICSI(intracytoplasmic sperm injection)was performed.
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Conclusion & Impact:Conclusion: Cumulus cell coculture can be used as a practical and easy method in patients for decreasing the percentage of fragmantation .
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Embriyo · Kısırlık · İleriye dönük çalışma

Abstract

Objective: Our aim was to investigate the effect of cumulus cell culture on fragmantation formation in human embriyos. Material and Methods: Twenty eight normal responder patients were involved in the study group. Following controlled ovarian hyperstimulation with rec FSH and pituitary desensitization with GnRH analogues, 344 mature oocytes were obtained and ICSI(intracytoplasmic sperm injection)was performed. Mature oocytes were randomly divided into control and study groups. Half of the mature oocytes were cocultured in cumulus cells for 72 hours in the study group. Results: When we compared two groups with respect to fragmantation percentage, there was statistically significant decrease in the percentage of fragmantation in cumulus cell cocultured Grade III embriyos. Conclusion: Cumulus cell coculture can be used as a practical and easy method in patients for decreasing the percentage of fragmantation .

Özet

Amaç: Çalışmamızda, kumulus hücre kültürünün insan embriyolarında fragmantasyon oranına etkilerini araştırmayı amaçladık. Gereç ve Yöntem: Çalışma grubuna kontrollü ovaryan stimulasyonuna iyi cevap veren 28 infertil hasta dahil edildi. Rekombinant FSH ile kontrollü ovaryan hiperstimülasyon ve GnRH analoğu ile pituiter desensitizasyon uygulanımı sonrası bu hastalardan toplanan 344 matür oosite ICSI(intrasitoplazmik sperm enjeksiyonu) uygulandı. Matür oositler rast gele iki gruba ayrılarak kontrol ve çalışma grupları oluşturuldu. Çalışma grubundaki matür oositlere 72 saat boyunca kumulus kokültürü yapıldı. Bulgular: Her iki grup fragmantasyon oranlarına göre karşılaştırıldığında , kumulus hücre kültürü yapılan grupta grade III (kötü kalitede) olan embriyolardaki fragmantasyon oranı , kontrol grubuna göre istatistiksel olarak anlamlı ölçüde düşük bulundu. Sonuç: Kumulus hücre kokültürünün pratik ve kolay uygulanabilir bir yöntem olması nedeniyle, ICSI sonrasında elde edilen embriyolarda fragmantasyonun azaltılması için kullanılmasını mümkün görmekteyiz.

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Cite This Article

KORKMAZ, C., CINCIK, M., AKAR, M., SELAM, F. B., BAYKAL, B. (2005). The effect of cumulus cell culture in the fragmantation formation of human embriyos. Medical Network Klinik Bilimler ve Doktor, Cilt: 11 - Sayı: 6, 641–647. https://ajindex.com/articles/278415

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