Journals / KSÜ Doğa Bilimleri Dergisi / 2017 / Cilt: 20 - Sayı: 3

Genotoxic Effects of the Essential Oil Obtained from Satureja hortensis Against to Hordeum Vulgare L. Seedlings

Satureja hortensis Bitkisinin Uçucu Yağlarının Hordeum Vulgare L. Tohumları Üzerine Genotoksik Etkileri

Pages
185–192
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results indicated that the third (0.4 µL mL-1) dose has changed the genomic stability about 46.16 % and remaining doses changed the genomic stability over the 50 %.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Plants use in many fields has increased significantly with the increasing of biotechnological methodsfrom past to present.
📊
Key Findings & Data:The oils were applied in four different doses (0.2, 0.4, 0.8 and 1.6 µL mL-1).
💡
Conclusion & Impact:Results indicated that the third (0.4 µL mL-1) dose has changed the genomic stability about 46.16 % and remaining doses changed the genomic stability over the 50 %.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Plants use in many fields has increased significantly with the increasing of biotechnological methodsfrom past to present. Their secondary metabolites are commonly used in many fields from medicine to agriculture. Because of their easy degradation, they will be used as an alternative to the synthetic chemicals. Deciding their proper non-phytotoxic doses for cereals will secure their safe usage. The present study aimed to determine the potential biopesticide activity of plants natural components in weeds. Thus, the essential oils obtained from Satureja hortensis species of Lamiaceae family were subjected to analysis of volatiles and applied to Hordeum vulgare L. seedlings. Potential genotoxic effects of these components on the seedlings were determined by RAPD (Randomly Amplified Polymorphic DNA) PCR. The oils were applied in four different doses (0.2, 0.4, 0.8 and 1.6 µL mL-1). Results indicated that the third (0.4 µL mL-1) dose has changed the genomic stability about 46.16 % and remaining doses changed the genomic stability over the 50 %. The effects of the essential oils on root and stem length of barley seedlings were varied. The oils have increased the root length and inhibited stem length in all doses compared to the control. It was concluded that plant essential oil components showed similar effects of synthetic chemicals in the literature. With the exception of the 0.4 µL mL-1, which may be phytotoxic in Hordeum vulgare L. seeds, all doses tested in the study have the potential to be used as bio pesticides against weeds

Özet

Bitkilerin, geçmişten günümüze artan biyoteknolojik yöntemler sayesinde kullanımları oldukça artmıştır. Sahip oldukları sekonder metabolitlerin tıptan tarıma birçok alanda kullanımı hızla yaygınlaşmaktadır. Özellikle kolay metabolize olmaları tarımsal alanlarda sentetik kimyasallar yerine kullanımlarının önünü açmıştır. Tahıl grubundaki bitkilere karşı fitotoksik olmayan dozlarının belirlenmesi güvenli bir şekilde kullanımlarını sağlayacaktır. Bu amaçla; mevcut çalışmada Lamiaceae bitki familyasına ait Satureja hortensis türünün uçucu yağlarının biyolojik aktiviteleri belirlenmeye çalışılmıştır. Türden elde edilen uçucu yağlar, içerik analizleri yapıldıktan sonra Hordeum vulgare L., bitkisinin çimlenen tohumlarına uygulanmış; muhtemel genotoksik etkileri RAPD (Randomly Amplified Polymorphic DNA) PCR ile ölçülmüştür. Dört farklı dozda (0.2, 0.4, 0.8 ve 1.6 µL mL-1) uygulanan bileşenlerin; 0.4 µL mL-1 dozunda genomik stabiliteyi % 46.16'ya düşürdüğü diğer dozlarda ise % 50'nin üzerinde tuttuğu gözlenmiştir. Ayrıca uçucu yağların; tüm dozlarda çimlenen arpa tohumlarının kök uzunluklarını kontrole göre arttırdığı, gövde uzunluklarında ise kontrole göre önemli sayılabilecek inhibisyonlara neden olduğu belirlenmiştir. Sonuç olarak bitki bileşenlerinin literatürde geçen sentetik kimyasalların gösterdiği etkiye benzer aktivite gösterdikleri belirlenmiştir. Hordeum vulgare L. tohumlarında fitotoksik sayılabilecek 0.4 µL mL-1 dozu dışındaki diğer dozların yabancı bitkilere karşı biyopestisit olarak kullanılabilme potansiyeli ortaya çıkmıştır

🔖

Cite This Article

BOZARI, S., ÇAKMAK, B., KURT, H. (2017). Genotoxic Effects of the Essential Oil Obtained from Satureja hortensis Against to Hordeum Vulgare L. Seedlings. KSÜ Doğa Bilimleri Dergisi, Cilt: 20 - Sayı: 3, 185–192. https://ajindex.com/articles/274554

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (7)

  1. Agar G, Aksakal O, Bozarı S, Sunar S, Erturk FA, Yildirim N, Sevsay S 2010. Genetic variation within and among three populations of Vicia canescens L. (Fabaceae) as revealed by RAPD and FAMEs analysis. Romanian Biotechnological Letters, 15 (4):5384-5391.
  2. Aksakal O, Erturk FA, Sunar S, Bozarı S, Agar G 2013. Assessment of genotoxic effects of 2, 4- dichlorophenoxyacetic acid on maize by using RAPD analysis. Industrial Crops and Products, 42:552-557.
  3. Aslan I, Özbek H, Çalmaşur Ö, Şahı̇n F 2004. Toxicity of essential oil vapours to two greenhouse pests, Tetranychus urticae Koch and Bemisia tabaci Genn. Industrial Crops and Products, 19 (2):167-173.
  4. Azhdarzadeh F, Hojjati M 2016. Chemical Composition and Antimicrobial Activity of Leaf, Ripe and Unripe Peel of Bitter Orange (Citrus aurantium) Essential Oils. Nutrition And Food Sciences Research, 3 (1):43-50.
  5. Basile A, Cobianchi RC, Rigano D, Senatore F, Bruno M, Rosselli S, Conte B, Sorbo S 2011. Potential allelopathic activity of Sideritis italica (Miller) Greuter et Burdet essential oil. Plant Biosystems, 145 (1):241-247. Bozarı S 2012. Lamiaceae Familyasına Ait Farklı Türlerden Elde Edilen Allelopatik Potansiyele Sahip Esansiyel Yağların Genotoksik Etkilerinin Belirlenmesi. Atatürk Universitesi, FBE, Moleküler Biyoloji ABD, Doktora Tezi, 148 s. Bozarı S, Agar G, Aksakal O, Erturk FA, Yanmis D 2013. Determination of chemical composition and genotoxic effects of essential oil obtained from Nepeta nuda on Zea mays seedlings. Toxicology and Industrial Health, 29 (4):339-348.
  6. Bozarı S, Aksakal O 2013. Application of random amplified polymorphic DNA (RAPD) to detect genotoxic effect of trifluralin on maize (Zea mays). Drug and chemical toxicology, 36 (2):163- 169. Cenkci S, Yildiz M, Cigerci IH, Konuk M, Bozdag A 2009. Toxic chemicals-induced genotoxicity detected by random amplified polymorphic DNA (RAPD) in bean (Phaseolus vulgaris L.) seedlings. Chemosphere, 76 (7):900-906.
  7. Currie R, Geier P 2016. Weed Control and Crop Injury with Single or Sequential Herbicide Applications in Grain Sorghum. Kansas Agricultural Experiment Station Research Reports, 2 (7):26. de Oliveira AS, Llanes LC, Brighente IMC, Nunes RJ, Yunes RA, Junior NM, Baumgart AMK, Aust AN, Cruz AB 2016. New Sulfonamides Derived from Carvacrol: Compounds with High Antibacterial Activity against Resistant Staphylococcus aureus Strains. Journal of Biosciences and Medicines, 4 (7):105-114. Facchini PJ 2001. Alkaloid biosynthesis in plants: biochemistry,