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Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2016 / Cilt: 22 - Sayı: 2

Köpek Olfaktorik Mukozasindan Olfaktorik Kök Hücrelerin Izolasyonu ve Karakterizasyonu

Isolation and Characterization of Olfactory Stem Cells from Canine Olfactory Mucosa

Korhan ALTUNBAŞ
Mustafa Volkan YAPRAKÇI
Sefa ÇELIK
Pages
237–243
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Olfactory mucosa of canine is a suitable alternative source of stem cells and can be applied to cell therapy in neurodegenerative diseases.

📌 3 Key Takeaways

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Objective & Hypothesis:Olfactory stem cells have great potential in the treatment of neurodegenerative diseases and they are good candidates for cell therapy due to the easy accessibility of olfactory mucosa.
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Key Findings & Data:When grown on poly-Dlysine with a serum-free culture medium supplemented with EGF (50 ng/ml) and FGF2 (50 ng/ml), olfactory stem cells gave rise to neurospheres.
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Conclusion & Impact:Olfactory mucosa of canine is a suitable alternative source of stem cells and can be applied to cell therapy in neurodegenerative diseases.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Olfactory stem cells have great potential in the treatment of neurodegenerative diseases and they are good candidates for cell therapy due to the easy accessibility of olfactory mucosa. The main objectives of this study were isolation, proliferation and characterization of olfactory mucosa stem cells that were further differentiated into olfactory neurospheres derived cells. When grown on poly-Dlysine with a serum-free culture medium supplemented with EGF (50 ng/ml) and FGF2 (50 ng/ml), olfactory stem cells gave rise to neurospheres. When grown in serum-containing culture medium newly plated spheres gave rise to olfactory neurosphere derived cells. Gene expression analysis revealed that, OCT4, SOX2, Nanog, Nestin, ?-tubulin and NCAM were expressed in olfactory stem cells. While the mRNA expressions of Nanog, Nestin, Oct4, ?III-tubulin and NCAM were downregulated in neurospheres, the mRNA expression of SOX2 upregulated in neurospheres. According to the gene levels of neurospheres generated from olfactory stem cells, beta tubulin and NCAM gene expressions were upregulated, whereas OCT4, Nanog, Sox2 and Nestin mRNA expressions were downregulated in Olfactory neurospheres derived cells. Olfactory mucosa of canine is a suitable alternative source of stem cells and can be applied to cell therapy in neurodegenerative diseases.

Özet

Olfaktorik kök hücreler nörodejeneratif hastalıkların tedavisinde büyük bir potansiyele sahiptir ve olfaktorik mukozaya kolay erişilebilirliği sayesinde hücre tedavisi için uygun bir adaydır. Bu çalışmanın amacı olfaktorik nörosfer kaynaklı hücrelere kadar farklılaştırılabilen olfaktorik kök hücrelerin izolasyonu, proliferasyonu ve karakterizasyonudur. Olfaktorik kök hücreler EGF (50 ng/ml) ve FGF (50 ng/ml) içeren serumsuz kültür vasatı içerisinde kültüre edildiğinde nörosferleri oluşturdular. Serum içeren kültür vasatında tekrar kültüre edildiklerinde olfaktorik nörosfer kaynaklı hücreleri şekillendirdiler. Gen ekspresyon analizleri OCT4, SOX2, Nanog, Nestin, ?-tubulin ve NCAM genlerinin olfaktorik kök hücrelerinde eksprese olduğunu ortaya çıkardı. Nanog, Nestin, Oct4, ? tubulin ve NCAM gen ekspresyonları nörosferlerde downregüle olurken, SOX2 geni upregüle oldu. Olfaktorik kök hücrelerin oluşturduğu nörosferlerin gen seviyeleri ile karşılaştırıldığında olfaktorik nörosfer kaynaklı hücrelerde ? tubulin ve NCAM gen ekspresyonları upregüle olurken OCT4, Nanog, Sox2 and Nestin mRNA ekspresyonları downregüle oldu. Köpeğin olfaktorik mukozası uygun alternatif bir kök hücre kaynağıdır ve köpek nörodejeneratif hastalıklarında hücre tedavisi için uygulanabilir.

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Cite This Article

ALTUNBAŞ, K., YAPRAKÇI, M. V., ÇELIK, S. (2016). Köpek Olfaktorik Mukozasindan Olfaktorik Kök Hücrelerin Izolasyonu ve Karakterizasyonu. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 22 - Sayı: 2, 237–243. https://ajindex.com/articles/274137

References (2)

  1. Brann JH, Firestein SJ: A lifetime of neurogenesis in the olfactory system. Front Neurosci, 8, 182, 2014. DOI: 10.3389/Fnins.2014.00182 Schwarz SC, Schwarz J: Translation of stem cell therapy for neurological diseases. Transl Res, 156, 155-160, 2010. DOI: 10.1016/J.Trsl.2010.07.002 Einstein O, Karussis D, Grigoriadis N, Mizrachi-Kol R, Reinhartz E, Abramsky O, Ben-Hur T: Intraventricular transplantation of neural pre- cursor cell spheres attenuates acute experimental allergic encephalomyelitis. Mol Cell Neurosci, 24, 1074-1082, 2003. DOI: 10.1016/J.MCN.2003.08.009 Lacorazza HD, Flax JD, Snyder EY, Jendoubi M: Expression of human beta-hexosaminidase alpha-subunit gene (the gene defect of Tay-Sachs disease) in mouse brains upon engraftment of transduced progenitor cells. Nat Med, 2, 424-429, 1996. DOI: 10.1038/Nm0496-424 Ourednik J, Ourednik V, Lynch WP, Schachner M, Snyder EY: Neural stem cells display an inherent mechanism for rescuing dysfunctional neurons. Nat Biotechnol, 20, 1103-1110, 2002. DOI: 10.1038/Nbt750 Pluchino S, Quattrini A, Brambilla E, Gritti A, Salani G, Dina G, Galli R, Del Carro U, Amadio S, Bergami A, Furlan R, Comi G, Vescovi AL, Martino G: Injection of adult neurospheres induces recovery in a chronic model of multiple sclerosis. Nature, 422, 688-694, 2003. DOI: 10.1038/Nature01552 Snyder EY, Taylor RM, Wolfe JH: Neural progenitor cell engraftment corrects lysosomal storage throughout the MPS VII mouse brain. Nature,, 367-370, 1995. DOI: 10.1038/374367a0 Chung DJ, Wong A, Hayashi K, Yellowley CE: Effect of hypoxia on generation of neurospheres from adipose tissue-derived canine mesenchymal stromal cells. Vet J, 199, 123-130, 2014. DOI: 10.1016/J. Tvjl.2013.10.020 Girard SD, Deveze A, Nivet E, Gepner B, Roman FS, Feron F: Isolating nasal olfactory stem cells from rodents or humans. J Vis Exp, 54, e2762, DOI: 10.3791/2762 Alves FR, Guerra RR, Fioretto ET, Delgado JC, Machado AAN, Ambrosio CE, Kerkis I, Miglino MA: Establishment of a protocol for obtention of neuronal stem cells lineages from the dog olfactory epithelium. Pesquisa Veterinaria Brasileira, 30, 363-372, 2010. DOI: 10.1590/ S0100-736X2010000400014 AD Veron MM, C Bienboire-Frosini, D Royer, P Asproni, A Cozzi, S D Girard, M Khrestchatisky, FS Roman, P Pageat: Are nasal stem cells a promising approach in geriatric veterinary medicine? In, IRSEA Institute of Research in Semiochemistry and Applied Ethology, International Congress: Marseille, France, 2014.
  2. Antoine Veron CB-F, Man