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Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2016 / Cilt: 22 - Sayı: 2

Sığır-Yak Melezlerinde Azoospermia-Benzeri (DAZL) Gen mRNA Ekspresyon Düzeyi ile İnfertilite Arasındaki İlişki Üzerine Bir Araştırma

An Investigation on the Relationship between the AzoospermiaLike (DAZL) Gene mRNA Expression and the Infertility in Male Cattle-Yak

Yincang HAN
Yong FU
Pages
287–289
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Therefore, the low expression level of DAZL gene might result in male cattle-yak infertility.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study was conducted to study the relationship between the infertility of male cattle-yak and the expression level of DAZL gene mRNA.
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Key Findings & Data:Real-time quantitative PCR analysis indicated that the expression levels of DAZL gene in cattle and yak testis were higher than its in cattle-yak, also cattle-yak and yak were significantly differet than cattle, respectively (P<0.05).
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Conclusion & Impact:Therefore, the low expression level of DAZL gene might result in male cattle-yak infertility.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

This study was conducted to study the relationship between the infertility of male cattle-yak and the expression level of DAZL gene mRNA. The expression profiles were obtained by RT-PCR. The DAZL gene was specifically expressed in cattle, yak and cattle-yak testis tissues,which confirmed its important role in the progression of cell cycle. Real-time quantitative PCR analysis indicated that the expression levels of DAZL gene in cattle and yak testis were higher than its in cattle-yak, also cattle-yak and yak were significantly differet than cattle, respectively (P<0.05). Therefore, the low expression level of DAZL gene might result in male cattle-yak infertility.

Özet

Bu çalışma erkek sığır-yak melezlerinde Azoospermia-Benzeri (DAZL) gen mRNA ekspresyon düzeyi ile infertilite arasındaki ilişkiyi araştırmak amacıyla yapılmıştır. Ekspresyon seviyeleri RT-PCR ile belirlendi. DAZL geninin spesifik olarak sığır, yak ve sığır-yak melezi testis dokularında eksprese edilmesi hücre siklusunun ilerlemesinde önemli rolü olduğunu teyit etmekteydi. Gerçek Zamanlı kantitatif PCR analizi sığır ve yak testis dokularında DAZL gen ekspresyon düzeyinin sığır-yak melezlerindekinden daha fazla olduğunu ve sığır-yak melezi ve yaklardaki seviyelerin anlamlı derecede sığırlardan farklı olduğunu göstermekteydi (P<0.05). Sonuç olarak, düşük DAZL gen ekspresyonu erkek sığır-yaklarda infertiliteye neden olabilir.

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Cite This Article

HAN, Y., FU, Y. (2016). Sığır-Yak Melezlerinde Azoospermia-Benzeri (DAZL) Gen mRNA Ekspresyon Düzeyi ile İnfertilite Arasındaki İlişki Üzerine Bir Araştırma. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 22 - Sayı: 2, 287–289. https://ajindex.com/articles/273612

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