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Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2009 / Cilt: 15 - Sayı: 4

Some kinetic properties of agrinase in sheep spleen tissue

Koyun dalak doku arginazının bazı kinetik özellikleri

FATİH MEHMET KANDEMİR
NECMİ ÖZDEMİR
Pages
553–559
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Furthermore, it was found that arginase in sheep spleen tissue was sensitive to different metal ions.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, some kinetic properties of arginase in the sheep spleen tissue was been optimized at first time in our laboratory.
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Key Findings & Data:Its was found to be preincubation temperature 58°C, preincubation period 13 min, incubation period 10 min and optimum pH 9.5 for sheep spleen tıssues.
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Conclusion & Impact:Furthermore, it was found that arginase in sheep spleen tissue was sensitive to different metal ions.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:arginaz · enzim aktivitesi · koyun

Abstract

In this study, some kinetic properties of arginase in the sheep spleen tissue was been optimized at first time in our laboratory. The spleen tissues used in the present study were obtained from the Elkas sloughterhause in Elazıg. The thiosemicarbazide- diacetylmonoxime urea (TDMU) method was used to measure arginase activity. Protein was measured by the method of Lowry et al. Its was found to be preincubation temperature 58°C, preincubation period 13 min, incubation period 10 min and optimum pH 9.5 for sheep spleen tıssues. Furthermore, it was found that arginase in sheep spleen tissue was sensitive to different metal ions. It was found that the most effective was Mn+2 from among (Mn+2, Cu+2, Mg+2, Ba+2, Fe+3, Ag+1, Cr+3, Sn+2, Hg+2, Pb+2, Co+2, Ni+2, Zn+2, Cd+2, Li+1, K+1, Al+3, Mo+6, Na+1 and Ca+2) metal ions. Arginase showed the highest activity in a 2 mM MnCl2 concentration. The enzyme has no activity in presence of Pb+2 and Ag+1 ions. Km of arginase in sheep spleen tissue for arginine were determined tobe 6 mM.

Özet

Bu çalışma ile koyun dalak doku arginazının bazı kinetik özellikleri ilk kez laboratuarımızda optimize edilmiştir. Çalışmada kullanılan dalak dokuları Elazığ'daki Elkas kesimevinden temin edilmiştir. Arginaz aktivitesini ölçmede tiyosemikarbaziddiasetilmonoksim üre (TDMU) metodu kullanılmıştır. Protein Lowry ve arkadaşlarının metoduna göre ölçülmüştür. Koyun dalak doku arginazı için preinkübasyon ısısı 58°C, preinkübasyon zamanı 13 dak., inkübasyon zamanı 10 dak. ve optimum pH 9.5 olarak bulunmuştur. Ayrıca koyun dalak doku arginazının çeşitli metal iyonlarına karşı duyarlılığı tespit edilmiş ve metal iyonları (Mn+2, Cu+2, Mg+2, Ba+2, Fe+3, Ag+1, Cr+3, Sn+2, Hg+2, Pb+2, Co+2, Ni+2, Zn+2, Cd+2, Li+1, K+1, Al+3, Mo+6, Na+1 ve Ca+2) içinde enzim aktivitesi üzerine en etkili metal iyonunun Mn+2 olduğu bulunmuştur. Enzim 2 mM'lık MnCl2 derişiminde en yüksek aktiviteyi göstermiştir. Pb+2 ve Ag+1 iyonları varlığında enzim hiç aktivite göstermemiştir. Koyun dalak doku arginazının arginin için Km’i 6 mM olarak saptanmıştır.

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Cite This Article

KANDEMİR, F. M., ÖZDEMİR, N. (2009). Some kinetic properties of agrinase in sheep spleen tissue. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 15 - Sayı: 4, 553–559. https://ajindex.com/articles/273452

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