Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2010 / Cilt: 16 - Sayı: 3

Diagnosis of Campylobacteriosis in the aborted bovine foetuses by pathological, immunohistochemical, microbiological and Real Time PCR

Atık sığır fetüslerinde kampilobakteriozisin patolojik immunohistokimyasal mikrobiyolojik ve gerçek zamanlı PZR ile teşhisi

Pages
509–514
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It is concluded that 6.6% (7/105) of abortion cases were caused by Campylobacteriosis and Real Time PCR more sensitive and usefull for the the diagnosis of the disease and immunohistochemical method is as sensitive as microbiologic methods for diagnosis in unsufficient labarotories.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study; 105 aborted bovine fetuses were examined for Campylobacteriosis and pathologic findings were compared with microbiologic, immunohistochemical and real time polimerase chain reaction (Real Time-PCR) methods.
📊
Key Findings & Data:Although, Campylobacter spp. was microbiologically detected in only 4 cases, this was detected in 5 cases by immunohistochemical method and by Real Time-PCR in 7 cases.
💡
Conclusion & Impact:It is concluded that 6.6% (7/105) of abortion cases were caused by Campylobacteriosis and Real Time PCR more sensitive and usefull for the the diagnosis of the disease and immunohistochemical method is as sensitive as microbiologic methods for diagnosis in unsufficient labarotories.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:bağışıksal tanı · histopatoloji · polimeraz zincir reaksiyonu

Abstract

In this study; 105 aborted bovine fetuses were examined for Campylobacteriosis and pathologic findings were compared with microbiologic, immunohistochemical and real time polimerase chain reaction (Real Time-PCR) methods. Although, Campylobacter spp. was microbiologically detected in only 4 cases, this was detected in 5 cases by immunohistochemical method and by Real Time-PCR in 7 cases. It is concluded that 6.6% (7/105) of abortion cases were caused by Campylobacteriosis and Real Time PCR more sensitive and usefull for the the diagnosis of the disease and immunohistochemical method is as sensitive as microbiologic methods for diagnosis in unsufficient labarotories.

Özet

Bu çalışmada toplam 105 atık sığır fetüsü kampilobakteriozis yönünden incelenerek hastalığın tanısında uygulanan patolojik bulgular ile imunohistokimyasal, mikrobiyolojik ve gerçek zamanlı polimeraz zincir reaksiyonu (Gerçek zamanlı PZR) yöntemlerine ait sonuçlar karşılaştırmalı olarak değerlendirildi. Yapılan incelemelerde, atıklardan 7’sinde gerçek zamanlı PZR ile, 5’inde immunohistokimyasal yöntemle pozitif reaksiyon tespit edilirken yalnızca 4’ünden mikrobiyolojik olarak Camphylobacter ssp. izole edildi. Sonuç olarak çalışmadaki atık olgularının %6.6’sının (7/105) kampilobakteriozisten kaynaklandığı ve gerçek zamanlı PZR’nin hastalığın tanısında daha güvenilir sonuç verdiği; buna karşın alt yapısı bulunmayan laboratuvarlarda immunoperoksidaz yöntemin de en az mikrobiyolojik yöntemler kadar duyarlı olduğu ve tanıda kullanılabileceği kanısına varıldı.

🔖

Cite This Article

TUZCU, M., ORUÇ, E., TUZCU, N., YOLDAŞ, A., YIĞIN, A. (2010). Diagnosis of Campylobacteriosis in the aborted bovine foetuses by pathological, immunohistochemical, microbiological and Real Time PCR. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 16 - Sayı: 3, 509–514. https://ajindex.com/articles/273377

References (1)

  1. Peter AT: Abortions in dairy cows: New insights and economic impact. Adv Dairy Technol, 12, 233-244, 2000. 2.Kirkbride CA: Bacterial agents detected in a 10-year study of bovine abortions and stillbirths. J Vet Diagn Invest, 5 (1): 64-68, 1993. 3.Ünver A, Erdoğan HM, Atabay Hİ, Şahin, Güneş V, Çitil M, Gökçe Hİ: Sığır attıklarından izole edilen brucella türlerinin RAPD-PCR ile genotiplendirilmesi. Kafkas Univ Vet Fak Derg, 12 (2): 121-127, 2006. 4.Sağlam YS, Türkütanıt SS, Taştan R, Bozoğlu H, Otlu S: Kuzeydoğu Anadolu Bölgesinde görülen bakteriyel sığır ve koyun abortlarının etiyolojik ve patolojik yönden incelenmesi. Selçuk Univ Vet Bil Derg, 14 (2): 133-145, 2002. 5.Campero MC, Anderson ML, Walker RL, Blanchard PC, Barbano L, Chlu P, Martinez A, Combessies G, Bardon JC, Cordeviola J: Immunhistochemical identification of Campylobacter fetus in natural cases bovine and ovine abortion. J Vet Med B, 52, 138-141, 2005. 6.Hazıroğlu R, Milli Ü: Veteriner Patoloji. Cilt II, Tamer Matbaacılık, Ankara. 1998. 7.Larson BL: Diagnosing the cause of bovine abortions and other perinatal deaths. Vet Med, 478-486, 1996 8.Jeffrey M, Hogg RA: Concurrent bovine virus diarrhoea virus and Campylobacter fetus infection in an aborted bovine fetus. Vet Rec, 4, 89-90, 1988. 9.Saurwein RW, Bisseling J, Horrevorts MA: Septic abortion associated with Campylobacter fetus supspecies fetus infection. Infection, 21, 331-335, 1993. 10.Hoffer MA: Bovine campylobacteriosis. Can Vet J, 22, 327-330, 1981 11.Jerret IV, McOrist S, Waddington J, Browning JW, Malecki JC, McCausland IP: Diagnostic studies of fetus, placenta and maternal blood from 265 bovine abortion. J Vet Diagn Invest, 4 (2): 175-180, 1992. 12.Donkersgoed J, Janzen ED, Chirino-Trejo M, Berry C, Clark EG, Haines DM: Campylobacter jejuni abortions in two beef cattle herds in Saskatchewan. Cornell Vet, 74 (1): 8-20, 1984. 13.Anderson ML, Blanchard CP, Barr CB, Hoffman LR: A survey of causes of bovine abortion occurring in the San Joaquin Valley, California. J Vet Diagn Invest, 2, 283-287, 1990. 14.Muz A, Ertaş HB, Öngör H, Gülcü HB, Özer H, Eröksüz H, Dabak M, Başbuğ O, Kalender H: Elaznğ ve çevresinde koyun ve keçilerde abortus olgularının bakteriyolojik serolojik ve patolojik olarak incelenmesi. Tr J Vet Anim Sci, 23 (1): 177-188, 1999. 15.Ak S, Turan N, Ak K, İleri K, Ilgaz A: Use of ELISA, IFA, and avidin-biotin staining for the diagnosis of bovine genital campylobacteriosis. Tr J Vet Anim Sci, 24, 113-121, 2000. 16.Pellegri