Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2010 / Cilt: 16 - Sayı: 3

Köpeklerde karma aşı ile aşılama sonrası hücresel ve humoral immun cevaplar üzerinde Aloe vera ve β-Glucan’ın immunostimülatör etkileri

Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs

Pages
405–412
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Additionally, it might have restorative effect on PLT counts.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to evaluate the effects of Aloe vera and β-Glucan on lymphocyte subsets, haematological parameters and immunglobulin concentrations following vaccination in dogs.
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Key Findings & Data:For this purpose, totally 20 street dogs were used.
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Conclusion & Impact:Additionally, it might have restorative effect on PLT counts.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:T lenfositleri · Aloe barbadensis · B lenfosit

Abstract

The aim of this study was to evaluate the effects of Aloe vera and β-Glucan on lymphocyte subsets, haematological parameters and immunglobulin concentrations following vaccination in dogs. For this purpose, totally 20 street dogs were used. The animals were allocated into five groups. Group 1 consisted of only polyvalent vaccine applied dogs. Group 2 and 3 were only Aloe vera and β-Glucan applied dogs, respectively. Group 4 and 5 were consisted of polyvalent vaccine in addition to Aloe vera and β-Glucan applied dogs, respectively. Blood samples were collected before vaccination and after vaccination (AV). Although platelet (PLT) counts in group 1 decreased AV, in all other groups (group 2, 3, 4, 5) it increased. White blood cell (WBC) and peripheral blood mononuclear lymphocyte (PBML) counts (in all groups), peripheral blood polymorphonuclear lymphocyte (PBPL) counts (group 1, 2, 4, 5), neutrophil (group 1, 4, 5), monocyte (group 2, 3, 5), packed cell volume (PCV) ratios (group 2 and 3) and haemoglobin (HGB) concentrations (group 3) increased AV. But, lymphocyte ratios in all groups did not change AV. CD8 T lymphocyte ratios in all groups increased at $7^{th}$ and $14^{th}$ days AV. Although CD3 T lymphocyte ratios in group 1 increased at $7^{th}$ day AV, CD4 T and B lymphocytes ratios did not change AV period. But, both CD3 and CD4 T lymphocytes ratios in all other groups increased at $7^{th}$ and $14^{th}$ days AV. Moreover, B lymphocytes ratios in all groups except for group 1 increased AV. On the other hand, B lymphocytes ratios in group 2 and 3 increased only $7^{th}$ day AV, while this increase in group 4 and 5 were present on both $7^{th}$ and $14^{th}$ days AV. Serum IgM concentrations in group 1 increased only $7^{th}$ day AV, whereas its levels did not change in group 2 and 3. Serum IgG concentrations in group 1, 2 and 3 increased only $14^{th}$ day AV. But, serum IgM and IgG concentrations in group 4 and 5 increased at $7^{th}$ and $14^{th}$ days AV. Administration of Aloe vera and β-Glucan after vaccination in dogs may stimulate both cellular and humoral immun responses. Additionally, it might have restorative effect on PLT counts.

Özet

Bu çalışmada köpeklerde aşılama sonrası lenfosit alt tipleri, hematolojik parametreler ve immunglobulin konsantrasyonları üzerinde Aloe vera ve β-Glucan’ın etkilerinin değerlendirilmesi amaçlandı. Yirmi sokak köpeği kullanıldı. Hayvanlar beş gruba ayrıldı. Grup 1’deki köpeklere sadece karma aşı uygulandı. Grup 2 ve 3’teki köpeklere sırasıyla sadece Aloe vera ve β-Glucan uygulandı. Grup 4 ve 5’teki köpeklere ise karma aşıya ilaveten Aloe vera ve β-Glucan uygulandı. Aşılama öncesi ve aşılama sonrası (AS) kan örnekleri alındı. Platelet (PLT) sayıları grup 1’de AS azalmasına rağmen, diğer tüm gruplarda (grup 2, 3, 4, 5) arttı. Total lökosit (WBC) ve periferal kan agranülosit (PBML) sayıları (tüm gruplarda), periferal kan granülosit (PBPL) sayıları (grup 1, 2, 4, 5), nötrofil (grup 1, 4, 5), monosit (grup 2, 3, 5) ve hematokrit (PCV) oranları (grup 2, 3) ile hemoglobin (HGB) konsantrasyonları (grup 3) AS’da arttı. Fakat, lenfosit oranları AS’da tüm gruplarda değişmedi. CD8 T lenfosit oranları tüm gruplarda AS 7 ve 14. günlerde arttı. CD3 T lenfosit oranları grup 1’de AS 7. günde artmasına rağmen, CD4 T lenfosit ve B lenfosit oranları AS periyotta değişmedi. Fakat, CD3 ve CD4 T lenfosit oranları diğer tüm gruplarda AS 7. ve 14. günlerde arttı. Üstelik, B lenfosit oranları AS’da grup 1 hariç diğer tüm gruplarda arttı. Ayrıca, B lenfosit oranları grup 2 ve 3’de sadece AS 7. günde artarken, grup 4 ve 5’de AS hem 7. hemde 14. günde arttı. Serum IgM konsantrasyonları grup 1’de sadece AS 7. günde artarken, grup 2 ve 3’de değişmedi. Serum IgG konsantrasyonları grup 1, 2 ve 3’de sadece AS 14. günde arttı. Fakat, serum IgM ve IgG konsantrasyonları grup 4 ve 5’de AS 7. ve 14. günde arttı. Köpeklerde aşılama sonrası Aloe vera ve β-Glucan uygulamasının hücresel ve hümoral immun cevapların her ikisini de stimüle edebileceği ve PLT sayıları üzerinde restoratif etkili olabileceği kanısına varıldı.

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Cite This Article

ALTUĞ, N., YÜKSEK, N., AĞAOĞLU, Z. T. (2010). Köpeklerde karma aşı ile aşılama sonrası hücresel ve humoral immun cevaplar üzerinde Aloe vera ve β-Glucan’ın immunostimülatör etkileri. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 16 - Sayı: 3, 405–412. https://ajindex.com/articles/273371

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