Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2010 / Cilt: 16 - Sayı: 4

Detection of Taylorella equigenitalis in Horses by means of bacteriological culture and PCR in Sanliurfa Region, Turkey

Şanlıurfa bölgesi’nde atlarda Taylorella equigenitalis’in kültür ve PCR yöntemi ile saptanması

Pages
605–609
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

PCR method is more sensitive than culture and it can be used for routine diagnosis.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study; swab samples (n: 780) of mares (n: 80) and stallions (n: 20) were analyzed in total of 200 througbred Arabian Horses from Sanliurfa Region.
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Key Findings & Data:Following molecular analyses; 5 (2.7% of mares) and 8 (4.4% mares) positive samples were found by direct PCR and culture-PCR, respectively.
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Conclusion & Impact:PCR method is more sensitive than culture and it can be used for routine diagnosis.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Taylorella equigenitalis · Türkiye · bakteriyoloji

Abstract

In this study; swab samples (n: 780) of mares (n: 80) and stallions (n: 20) were analyzed in total of 200 througbred Arabian Horses from Sanliurfa Region. The samples were analysed by culture, direct PCR and culture-PCR methods. Taylorella equigenitalis was notisolated after bacteriological examination of swab samples. Following molecular analyses; 5 (2.7% of mares) and 8 (4.4% mares) positive samples were found by direct PCR and culture-PCR, respectively. While five mares were positive by direct PCR and culture-PCR, three mares were only positive by culture-PCR. No T. equigenitalis detection was found in health mares and stallions. As a result; T. equigenitalis is a problem in Sanliurfa region. PCR method is more sensitive than culture and it can be used for routine diagnosis.

Özet

Bu çalışmada Şanlıurfa bölgesi’nde bulunan 180 kısrak ve 20 aygır olmak üzere toplam 200 adet safkan Arap atından alınan 780 adet svab örneği incelendi. Alınan örnekler kültür, direkt-PCR ve kültür-PCR yöntemleriyle değerlendirildi. Svab örneklerinin bakteriyolojik muayeneleri sonucunda Taylorella equigenitalis izole edilemedi. Moleküler inceleme sonucunda, direkt-PCR ile 5 (%2.7) kısrakta, kültür-PCR ile 8 (%4.4) kısrakta pozitiflik saptandı. Direkt PCR ve kültür PCR ile 5 kısrak pozitif bulunurken, 3 kısrak sadece kültür-PCR ile pozitif olarak saptandı. Sağlıklı kısraklar ve aygırlarda pozitiflik belirlenmedi. Sonuç olarak, Şanlıurfa yöresinde T. equigenitalis’in sorun olduğu ve PCR metodunun kültür yöntemine göre daha üstün ve rutin teşhiste uygulanabilir bir test olduğu kanısına varıldı.

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Cite This Article

TEL, O. Y., KESKİN, O., ZONTURLU, A. K., DAKMAN, A., YURDAYDIN, N. (2010). Detection of Taylorella equigenitalis in Horses by means of bacteriological culture and PCR in Sanliurfa Region, Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 16 - Sayı: 4, 605–609. https://ajindex.com/articles/273326

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