Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2011 / Cilt: 17 - Sayı: 0

Alpha-Amylase Production by Bacillus subtilis RSKK96 in Submerged Cultivation

Submerged Kültürü ile Bacillus subtilis RSKK96'dan ?-Amilaz Üretimi

Pages
17–22
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Maximum ?-amylase production (858.6±41.9 U/mg) was obtained in a medium containing 0.5% cotton stalk in 72 h.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The bacterial strain Bacillus subtilis RSKK96 was shown to produce extracellular ?-amylase.
📊
Key Findings & Data:Enzyme synthesis occurred at 72 h with an optimum of 37°C.
💡
Conclusion & Impact:Maximum ?-amylase production (858.6±41.9 U/mg) was obtained in a medium containing 0.5% cotton stalk in 72 h.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The bacterial strain Bacillus subtilis RSKK96 was shown to produce extracellular ?-amylase. Enzyme synthesis occurred at 72 h with an optimum of 37°C. Eff ects of various carbon, nitrogen, amino acid sources and chemicals on ?-amylase production were examined. FeSO4, ZnSO4 and CuSO4 inhibited bacterial growth as a result, amylase production. Maximum ?-amylase production (858.6±41.9 U/mg) was obtained in a medium containing 0.5% cotton stalk in 72 h.

Özet

Bakteriyel tür Bacillus subtilis RSKK96'nın ekstrasellüler ?-amilaz ürettiği gözlenmiştir. Enzim sentezi 72. saatte optimum 37°C'de elde edilmiştir. Çeşitli karbon, azot ve aminoasit kaynakları ve kimyasalların ?-amilaz üretimi üzerine etkileri incelenmiştir. FeSO4, ZnSO4ve CuSO4 bakteriyel büyümeyi ve dolayısıyla ?-amilaz üretmini inhibe etmiştir. Maksimum ?-amilaz üretimi (858.6±41.9 U/mg) %0.5 pamuk sapı bulunan ortamda 72. saatte elde edilmiştir.

🔖

Cite This Article

AKCAN, N., UYAR, F., GÜVEN, A. (2011). Alpha-Amylase Production by Bacillus subtilis RSKK96 in Submerged Cultivation. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 17 - Sayı: 0, 17–22. https://ajindex.com/articles/273230

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Gangadharan D, Nampoothiri KM, Sivaramakrishnan S, Pandey A: Biochemical characterization of raw-starch-digesting alpha amylase purified from Bacillus amyloliquefaciens. Appl Biochem Biotech, 158, 653-, 2009.
  2. Sivaramakrishnan S, Gangadharan D, Nampoothiri KM, Soccol CR, Pandey A: ?-Amylases from microbial sources-an Overview on recent developments. Food Technol Biotechnol, 44, 173-184, 2006.
  3. Gupta R, Gigras P, Mohapatra H, Goswami VK, Chauhan B: Microbial ?-amylases: A biotechnological perspective. Process Biochem, 38, 1599-, 2003
  4. Pandey A, Nigam P, Soccol CR, Soccol VT, Singh D, Mohan R: Advances in microbial amylases. Biotechnol Appl Biochem, 31,135-152, 2000.
  5. Burhan A, Nisa U, Gokhan C, Omer C, Ashabil A, Osman G: Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. isolate ANT- 6. Process Biochem, 38, 1397-1403, 2003. Bernfield P: Amylases, ? and ?. In, Methods in Enzymology, Vol. 1, pp. 158. Academic Press,
  6. New York, USA, 1955.
  7. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the folin-phenol reagents. J Biol Chem, 48, 17-25, 1951.
  8. Rasooli I, Astaneh SDA, Borna H, Barchini KM: Thermostable ?-amylase producing natural variant of Bacillus spp. isolated from soil in iran. Am J Agri Biol Sci, 3 (3): 591-596, 2008
  9. Nusrat A, Rahman SR: Comparative studies on the production of extracellular ?-amylase by three mesophilic Bacillus isolates. Bangladesh J Microbiol, 24 (2): 129-132, 2007.
  10. Lealem F, Gashe BA: Amylase production by a gram-positive bacterium isolated from fermenting tef (Eragrostis tef ). J Appl Microbiol, (3): 348-352, 2008.
  11. Ramesh MV, Lonsane BK: Solid state fermentation for production of ?-amylase by Bacillus megaterium 16M. Biotechnol Lett, 9 (5): 323-328, 1987.
  12. Albayrak N, Donmez S, Balk M: Toprak ve su örneklerinden amilolitik bakterilerin izolasyonu ve bazı enzimatik özelliklerinin belirlenmesi. Turk J Biology, 20, 47-54, 1996.
  13. Lin LL, Chyau CC, Hsu WH: Production and properties of a rawstarch- degrading amylase from the thermophilic and alkaliphilic Bacillus sp. TS- Biotechnol Appl Biochem, 28, 61-68, 1998.
  14. Kıran Ö, Çömlekçioğlu U, Arıkan B: Effects of carbon sources and various chemicals on the production of a novel amylase from a thermophilic Bacillus sp. K-12. Turk J Biol, 29, 99-103, 2005.
  15. Narang S, Satyanarayana T: Thermostable ?-amylase production by an extreme thermophile Bacillus thermooleovorans. Lett Appl Microbiol,, 31-35, 20