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Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2012 / Cilt: 18 - Sayı: 2

Türkiye’nin Erzurum ilinde insan tüketimi için satılan istavrit balıklarında (trachurus trachurus) anisakis pegreffii’nin moleküler tanısı

Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey

ARMAĞAN ERDEM ÜTÜK
Fatma Çiğdem PİŞKİN
İBRAHİM BALKAYA
Pages
303–307
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

With this study, A. pegreffii was molecularly detected for the first time in Turkey.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study was planned to identify six parasites detected in horse mackerels (Trachurus trachurus) sold for human consumption in Erzurum province.
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Key Findings & Data:Species identification was done by PCR and PCR-RFLP analysis of ITS (ITS-1, 5.8S subunit rRNA gene and ITS-2) region of ribosomal DNA (rDNA) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) and mt-CO2 genes.
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Conclusion & Impact:With this study, A. pegreffii was molecularly detected for the first time in Turkey.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Anisakis · teşhis · anisakiyaz

Abstract

This study was planned to identify six parasites detected in horse mackerels (Trachurus trachurus) sold for human consumption in Erzurum province. DNA extraction was performed on six parasites diagnosed as nematode. Species identification was done by PCR and PCR-RFLP analysis of ITS (ITS-1, 5.8S subunit rRNA gene and ITS-2) region of ribosomal DNA (rDNA) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) and mt-CO2 genes. According to the PCR and PCR-RFLP results, all parasites were identified as Anisakis pegreffii. Partial sequence of mt-CO1 gene of one randomly selected parasite was corresponding with A. simplex and mt-CO2 genes of two parasites were corresponding with A. pegreffii. With this study, A. pegreffii was molecularly detected for the first time in Turkey.

Özet

Bu çalışma, Erzurum ilinde insan tüketimi için satılan istavrit balıklarında (Trachurus trachurus) tespit edilen altı parazitin tür tanısını yapmak amacıyla planlanmıştır. Nematod olarak belirlenen altı parazitten DNA ekstraksiyonu yapılmıştır. Tür tanısı, rDNA’nın ITS bölgelerinin (ITS1, 5.8 alt ünite rRNA ve ITS2) PCR ve PCR-RFLP analizleri ve mitokondrial sitokrom c oksidaz alt ünite 1 (mt-CO1) ve mt- CO2 genlerinin kısmi olarak sekanslanması ile yapılmıştır. PCR ve PCR-RFLP sonuçlarına göre tüm parazitlerin Anisakis pegreffii olduğu belirlenmiştir. Rastgele seçilen bir parazitin mt-CO1 geninin kısmi sekans sonucu A. simplex, iki parazitin mt-CO2 geninin kısmi sekans sonuçları ise A. pegreffii ile uyuşmuştur. Bu çalışma ile A. pegreffii Türkiye’de ilk kez moleküler olarak tespit edilmiştir.

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Cite This Article

ÜTÜK, A. E., PİŞKİN, F. Ç., BALKAYA, İ. (2012). Türkiye’nin Erzurum ilinde insan tüketimi için satılan istavrit balıklarında (trachurus trachurus) anisakis pegreffii’nin moleküler tanısı. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 18 - Sayı: 2, 303–307. https://ajindex.com/articles/273103

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References (13)

  1. Lymbery AJ, Cheah FY: Anisakid nematodes and anisakiasis. In, Murrell KD, Fried B (Eds): Food-Borne Parasitic Zoonoses, Fish and Plant Borne Parasites. 1st ed., pp. 185-209, Springer Science Business Media, LLC, New York, USA, 2007
  2. Sterling CR: Food-borne nematod infection. In, Ortega YR (Ed): Food- borne Parasites. 1st ed., pp. 135-153, Springer Science Business Media, LLC, New York, USA, 2006
  3. Anderson RC: Nematode parasites of vertebrates: Their development and transmission. 2nd ed., pp. 245-347, Cambridge, CAB International, 1992
  4. Mattiucci S, Nascetti G: Molecular systematics, phylogeny and ecology of anisakid nematodes of the genus Anisakis Dujardin, 1845: An update. Parasité, 13, 99-113, 2006
  5. Umerha A, Kawakami Y, Araki J, Uchida A: Multiplex PCR for the identification of Anisakis simplex sensu stricto, Anisakis pegreffii and the other anisakid nematodes. Parasitol Int, 57, 49-53, 2008
  6. Fang W, Xu S, Zhang S, Wang Y, Chen X, Luo D: Multiple primer PCR for the identification of Anisakid nematodes from Taiwan Strait. Exp Parasitol, 124, 197-201, 2010
  7. Umerha A, Kawakami Y, Matsui T, Araki J, Uchida A: Molecular identification of Anisakis simplex sensu stricto and Anisakis pegreffii (Nematoda: Anisakidae) from fish and cetacean in Japanase waters. Parasitol Int, 55, 267-271, 2006
  8. Umerha A, Kawakami Y, Ooi HK, Uchida A, Ohmae H, Sugiyama H: Molecular identification of Anisakis type l larvae isolated from hairtail fish off the coasts of Taiwan and Japan, Int J Food Microbiol, 143, 161-165, 2010
  9. S´anchez JM, Artacho-Reinoso ME, D´iaz-Gavil´an M, Valero-L´opez A: Structure of Anisakis simplex s.l. populations in a region sympatric for A. pegreffii and A. simplex s.s. Absence of reproductive isolation between both species. Mol Biochem Parasitol, 141, 155-162, 2005
  10. Mattiucci S, Paoletti M, Borrini F, Palumbo M, Palmieri RM, Gomes V, Casati A, Nascetti G: First molecular identification of the zoonotic parasite Anisakis pegreffii (Nematoda: Ascaridida) in paraffin-embedded granuloma taken from a case of human intestinal anisakiasis in Italy. BMC Infect Dis, 11, 1-6, 2011
  11. Kim KH, Eom KS, Park JK: The complete mitochondrial genome of Anisakis simplex (Ascaridida: Nematoda) and phylogenetic implications. Int J Parasitol, 36, 319-328, 2006
  12. Sambrook J, Fritsch EF, Maniatis T: Molecular cloning: A laboratory manual, 2nd ed., Cold Spring Harbor, NY, 1989
  13. Utuk AE, Simsek S, Koroglu E, McManus DP: Molecular genetic cha