SavedAdd your journal

Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2012 / Cilt: 18 - Sayı: 5

Erkek ratlarda renal iskemi-reperfüzyon hasarına karşı elajik asitin koruyucu etkisi

The protective effect of ellagic acid against renal ischemia-reperfusion injury in male rats

Yaşar BOZKURT
Uğur FIRAT
Murat ATAR
Ahmet Ali SANCAKTUTAR
Necmettin PEMBEGUL
Haluk SÖYLEMEZ
Hatice YÜKSEL
Harun ALP
Mehmet Nuri BODAKÇI
Namık Kemal HATİPOĞLU
Sadık BÜYÜKBAŞ
Pages
823–828
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion; EA pretreatment ameliorated the oxidative damage and histopathological changes occurring following renal I/R injury.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study was to evaluate the possible protective effect of ellagic acid (EA) on rats following renal ischemia–reperfusion (I/R) injury.
📊
Key Findings & Data:Sham group underwent laparotomy then waited for 45 min without ischemia.
💡
Conclusion & Impact:In conclusion; EA pretreatment ameliorated the oxidative damage and histopathological changes occurring following renal I/R injury.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:hayvan modelleri · hastalık modelleri · ellagik asit

Abstract

The aim of this study was to evaluate the possible protective effect of ellagic acid (EA) on rats following renal ischemia–reperfusion (I/R) injury. Twenty-four Wistar rats were divided into three groups. Sham group underwent laparotomy then waited for 45 min without ischemia. I/R group were subjected to left renal ischemia for 45 minutes followed by 60 min of reperfusion. I/R+EA group were subjected to the same renal ischemia/reperfusion as the I/R group, were also given 85 mg/kg EA perorally 30 min prior to the ischemia. Malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) were determined on the blood samples and kidney tissues. Histopathological analyses were conducted on the kidney tissues. I/R damage significantly increased serum MDA levels in the I/R group when compared with Sham group. Serum TAC level was significantly lower in I/R group than I/R+EA group. A significantly increase on OSI levels and decrease on TAC levels was found in the kidneys in I/R group. In I/R + EA group, EA reversed the negative effects of I/R injury. EA pretreatment was effective in decreasing tubular necrosis score. In conclusion; EA pretreatment ameliorated the oxidative damage and histopathological changes occurring following renal I/R injury.

Özet

Bu çalışmanın amacı ratlarda renal iskemi-reperfüzyon (I/R) hasarına karşı elajik asitin (EA) olası koruyucu etkisini değerlendirmektir. Yirmi dört erkek Wistar rat; Sham, I/R ve I/R+EA olmak üzere üç gruba ayrıldı. Sham grubuna laparotomi yapıldı ve iskemi uygulanmaksızın 45 dakika bekletildi. I/R grubuna 45 dakikalık sol renal iskemiyi takiben 60 dakikalık reperfüzyon uygulandı. I/R+EA grubuna da I/R grubundakilere benzer şekilde iskemi/reperfüzyon prosedürü uygulandı, fakat iskemiden 30 dakika önce ratlara 85 mg/kg EA ağızdan verildi. Kanda ve böbrek dokusunda malondialdehit (MDA), total antioksidan kapasite (TAC), total oksidan statusu (TOS) ve oksidatif stres indeksi (OSI) bakıldı. Böbrek dokusu histopatolojik yönden incelendi. Sham grubu ile kıyaslandığında I/R grubunda serum MDA düzeyi anlamlı derecede yüksek idi. Ayrıca I/R+EA grubu ile kıyaslandığında I/R grubunda serum TAC düzeyi anlamlı derecede düşük bulundu. I/R grubunda böbrek dokusunda OSI düzeyi yüksek TAC düzeyi düşük bulunurken, I/R+EA grubunda iskemi ile oluşan etkilerin EA ile düzeldiği görüldü. EA verilmesinin tubuler nekroz skorunu azaltmada etkili olduğu bulundu. Çalışmanın sonucuna göre; EA, renal I/R hasarı sonrası meydana gelen oksidatif hasarı ve oluşan histopatolojik değişiklikleri iyileştirmektedir.

🔖

Cite This Article

BOZKURT, Y., FIRAT, U., ATAR, M., SANCAKTUTAR, A. A., PEMBEGUL, N., SÖYLEMEZ, H., YÜKSEL, H., ALP, H., BODAKÇI, M. N., HATİPOĞLU, N. K., BÜYÜKBAŞ, S. (2012). Erkek ratlarda renal iskemi-reperfüzyon hasarına karşı elajik asitin koruyucu etkisi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 18 - Sayı: 5, 823–828. https://ajindex.com/articles/273046

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Donnahoo KK, Meldrum DR, Shenkar R, Chung CS, Abraham E, Harken AH: Early renal ischemia, with or without reperfusion, activates NFkappaB and increases TNF-alpha bioactivity in the kidney. J Urol, 163, 1328-1332, 2000
  2. Sivarajah A, Chatterjee PK, Patel NS, Todorovic Z, Hattori Y, Brown PA, Stewart KN, Mota-Filipe H, Cuzzocrea S, Thiemermann C: Agonists of peroxisome-proliferator activated receptor-gamma reduce renal ischemia/ reperfusion injury. Am J Nephrol, 23, 267-276, 2003
  3. Parks DA and Granger DN: Contributions of ischemia and reperfusion to mucosal lesion formation. Am J Physiol 250, 749-753, 1986
  4. El-kossi A, Abdellah M, Rashad A, Hamed S: The effectiveness of evening primrose oil and alpha lipoic acid in recovery of nerve function in diabetic rats. J Clin Exp Invest, 2, 245-253, 2011
  5. Mejia-Meza EI, Yanez JA, Remsberg CM, Takemoto JK, Davies NM, Rasco B, Clary C: Effect of dehydration on raspberries: Polyphenol and anthocyanin retention, antioxidant capacity, and antiadipogenic activity. J Food Sci, 75, H5-12, 2010
  6. Hagiwara Y, Kasukabe T, Kaneko Y, Niitsu N, Okabe-Kado J: Ellagic acid, a natural polyphenolic compound, induces apoptosis and potentiates retinoic acid-induced differentiation of human leukemia HL-60 cells. Int J Hematol, 92, 136-143, 2010
  7. Edderkaoui M, Odinokova I, Ohno I, Gukovsky I, Go VL, Pandol SJ, Gukovskaya AS: Ellagic acid induces apoptosis through inhibition of nuclear factor kappa B in pancreatic cancer cells. World J Gastroenterol, 14, 3672-3680, 2008
  8. Whitley AC, Stoner GD, Darby MV, Walle T: Intestinal epithelial cell accumulation of the cancer preventive polyphenol ellagic acid-extensive binding to protein and DNA. Biochem Pharmacol, 66, 907-915, 2003
  9. Rogerio AP, Fontanari C, Melo MC, Ambrosio SR, de Souza GE, Pereira PS, Franca SC, da Costa FB, Albuquerque DA, Faccioli LH: Anti-inflammatory, analgesic and anti-oedematous effects of Lafoensia pacari extract and ellagic acid. J Pharm Pharmacol, 58, 1265-1273, 2006
  10. Falsaperla M, Morgia G, Tartarone A, Ardito R, Romano G: Support ellagic acid therapy in patients with hormone refractory prostate cancer (HRPC) on standard chemotherapy using vinorelbine and estramustine phosphate. Eur Urol, 47, 449-454, 2005
  11. Alp H, Aytekin I, Esen H, Basarili K, Kul S: Effects of caffeic acid phenethyl ester, ellagic acid, sulforaphane and curcumin on diazinon induced damage to the lungs, liver and kidneys in an acute toxicity rat model. Kafkas Univ Vet Fa