Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2013 / Cilt: 19 - Sayı: 1

Köpeklerde ovaryohisterektomi’nin HSP70 ve anti-HSP70 antikorları üzerine etkisi

The effect of canine ovariohysterectomy on HSP70 and anti-HSP70 antibodies

Pages
85–88
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result, the mean +/- SD concentration of HSP70 (4.86±0.99 ng/mL) and anti-HSP70 (109.77±16.64 ng/mL) antibodies in the dogs’ blood samples taken after ovariohysterectomy were found to be significantly lower when compared to the preoperative amounts.

📌 3 Key Takeaways

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Objective & Hypothesis:The present study aimed to determine the effects of ovariohysterectomy on blood serum concentrations of Heat Shock Protein (HSP) 70 and anti-HSP70 antibodies in dogs.
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Key Findings & Data:For this purpose, 87 female stray dogs were used as the materials.
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Conclusion & Impact:As a result, the mean +/- SD concentration of HSP70 (4.86±0.99 ng/mL) and anti-HSP70 (109.77±16.64 ng/mL) antibodies in the dogs’ blood samples taken after ovariohysterectomy were found to be significantly lower when compared to the preoperative amounts.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:cerrahi · cerrahi işlemler · köpek

Abstract

The present study aimed to determine the effects of ovariohysterectomy on blood serum concentrations of Heat Shock Protein (HSP) 70 and anti-HSP70 antibodies in dogs. For this purpose, 87 female stray dogs were used as the materials. Ten milliliters of blood was taken from the animals preoperatively and on the first day postoperatively. The concentration of extracellular HSP70 and anti-HSP70 antibodies in blood serums were measured by using commercial ELISA kits. Subsequently, the preoperatively and postoperatively obtained data were compared. As a result, the mean +/- SD concentration of HSP70 (4.86±0.99 ng/mL) and anti-HSP70 (109.77±16.64 ng/mL) antibodies in the dogs’ blood samples taken after ovariohysterectomy were found to be significantly lower when compared to the preoperative amounts.

Özet

Bu çalışmada köpeklerde kan serumu Heat Shock Protein (HSP) 70 ve anti-HSP 70 antikorlarının düzeyleri üzerine ovaryohisterektominin etkilerinin belirlenmesi amaçlanmıştır. Bu amaçla, 87 adet dişi sokak köpeği materyal olarak kullanıldı. Hayvanlardan operasyon öncesi ve sonrası 1. gün 10 ml kan örnekleri alındı. Kan serumlarındaki hücre dışı HSP70 ve anti-HSP70 antikorlarının miktarları ticari ELISA kitleri kullanılarak ölçüldü. Daha sonra operasyon öncesi ve sonrası elde edilen verilerin istatistikî analizleri yapıldı. Sonuç olarak; köpeklerde ovaryohisterektomi sonrası alınan kan numunelerindeki HSP70 (4.86±0.99 ng/ml) ve anti-HSP70 (109.77±16.64 ng/ml) antikorlarının miktarlarının operasyon öncesine göre daha düşük olduğu tespit edildi.

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Cite This Article

YÜKSEL, M., SAAT, N., RIŞVANLI, A. (2013). Köpeklerde ovaryohisterektomi’nin HSP70 ve anti-HSP70 antikorları üzerine etkisi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 19 - Sayı: 1, 85–88. https://ajindex.com/articles/272961

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References (13)

  1. Stone EA: Ovary and uterus. In, Slatter DH (Ed): Small Animal Surgery. 3rd ed., pp. 1487-1502, WB Saunders, Philadelphia, 2002
  2. Vabulas RM, Ahmad-Nejad P, Ghose S, Kirschning CJ, Issels RD, Wagner H: HSP70 as endogenous stimulus of the Toll/interleukin-1 receptor signal pathway. J Biol Chem, 277, 15107-15112, 2002. 3. van Eden W, van der Zee R, Prakken B: Heat-shock proteins induce T-cell regulation of chronic inflammation. Nat Rev Immunol, 5, 318-330, 2005
  3. Askar TK, Ergun N, Turunc V: Heat shock proteins and their physiological roles. Kafkas Univ Vet Fak Derg, 13, 109-114, 2007
  4. Dillmann WH: Heat shock proteins and protection against ischemic injury. Infect Dis Obstet Gynecol, 7, 55-71, 1999
  5. Kregel K: Molecular biology of thermoregulation: Invited review: Heat shock proteins: Modifying factors in physiological stress responses and acquired thermotolerance. J Appl Physiol, 92, 2177-2186, 2002
  6. Kimura F, Itoh H, Ambiru S, Shimizu H, Togawa A, Yoshidome H, Ohtsuka M, Shimamura F, Kati A, Nukui Y, Miyazaki, M: Circulating heat- shock protein 70 is associated with postoperative infection and organ dysfunction after liver resection. Am J Surg, 187, 777-784, 2004
  7. Lancaster G, Febbraio M: Mechanisms of stress-induced cellular HSP72 release: Implications for exercise-induced increases in extracellular HSP72. Exerc Immunol Rev, 11, 46-52, 2005
  8. Pockley AG, Wu R, Lemne C, Kiessling R, de Faire U, Frostegård J: Circulating heat shock protein 60 is associated with early cardiovascular disease. Hypertension, 36, 303-307, 2000
  9. Kelly S, Bieneman A, Horsburgh K, Hughes D, Sofroniew MV, McCulloch J, Uney JB: Targeting expression of hsp70i to discrete neuronal populations using the Lmo-1 promoter: Assessment of the neuro- protective effects of hsp70i in vivo and in vitro. J Cereb Blood Flow Metab, 21, 972-981, 2001
  10. Calderwood SK, Theriault JR, Gong J: Message in a bottle: Role of the 70-kDa heat shock protein family in anti-tumor immunity. Eur J Immunol 35, 2518-2527, 2005
  11. Cizkova D, Carmel JB, Yamamoto K, Kakinohana O, Sun D, Hart RP, Marsala M: Characterization of spinal HSP72 induction and development of ischemic tolerance after spinal ischemia in rats. Exp Neurol, 185, 97-108 2004
  12. Oglesbee MJ, Herdman AV, Passmore GG, Hoffman WH: Diabetic ketoacidosis increases extracellular levels of the major inducible 70-kDa heat shock protein. Clin Biochem, 38, 900-904, 2005
  13. Awad H, Suntres Z, Heijmans J, Smeak D, Bergdall-Costell